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Kit for prognostic stratification of AML (Acute Myelocytic Leukemia)

A technology of acute myeloid cells and kits, applied in the field of molecular detection, can solve the problems of inability to improve prognosis, poor prognosis, etc., and achieve rapid detection results

Pending Publication Date: 2017-05-24
ANNOROAD GENE TECH BEIJING +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the prognosis of AML patients is good, 40% of patients still relapse, and the long-term survival rate over 5 years is less than 50%. The recurrence rate of such patients is as high as 70% if they have c-kit mutation. Once relapse, the prognosis is extremely poor. Stem cell transplantation (allo-HSCT) also does not improve its prognosis

Method used

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  • Kit for prognostic stratification of AML (Acute Myelocytic Leukemia)
  • Kit for prognostic stratification of AML (Acute Myelocytic Leukemia)
  • Kit for prognostic stratification of AML (Acute Myelocytic Leukemia)

Examples

Experimental program
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Effect test

Embodiment 1

[0068] Synthesis of embodiment 1 fluorescently labeled primers

[0069] The following fluorescently labeled primers were synthesized by Shanghai Jierui Company.

[0070]

[0071]

[0072] In the table, F or FS indicates the upstream primer, and R or RS indicates the downstream primer.

Embodiment 2

[0073] Example 2 Detection by PCR and multiple fluorescent capillary gel electrophoresis

[0074] 1) Extraction of total RNA in blood

[0075] a. Take 200 μL of blood, add 1 mL of Trizol (Invitrogen), shake vigorously to mix, and place at room temperature for 10 minutes;

[0076] b. Add 200 μL of chloroform to a, mix vigorously; leave at room temperature for 5 minutes;

[0077] c. 12000rpm, centrifuge at 4°C for 15 minutes;

[0078] d. Aspirate the supernatant into another new 1.5mL centrifuge tube;

[0079] e. Add an equal volume of isopropanol to d and let stand at room temperature for 30 minutes;

[0080] f.12000rpm, centrifuge at 4°C for 15 minutes; discard the supernatant;

[0081] g. Wash the precipitate with 500 μL of 75% ethanol, and wash twice;

[0082] h. with 20μLddH 2 O (no RNase) dissolves the precipitate;

[0083] i. Electrophoresis to detect the integrity of the extracted RNA, and use Qubit or Nanodrop to measure the concentration of RNA.

[0084] 2) Rev...

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Abstract

The invention provides a kit for the prognostic stratification of AML (Acute Myelocytic Leukemia), which is capable of quickly detecting multigene / multisite mutation. By using the kit, a mutant site can be completely detected; further, the detection sensitivity is high; the entire experimental process from the preparation of a sample to the issue of a report can be completed within one day; the detection is quick and the detection result is accurate; the coverage degree is high; meanwhile, the quick guidance can be effectively provided for the prognostic analysis of the AML.

Description

technical field [0001] The invention relates to a reagent kit that can be used for the stratification of prognosis of acute myeloid leukemia, and belongs to the technical field of molecular detection. Background technique [0002] Acute myelocytic leukemia (AML) is a malignant clonal disease of blood with significant heterogeneity. Even if the prognosis of AML patients is good, 40% of patients still relapse, and the long-term survival rate over 5 years is less than 50%. The recurrence rate of such patients is as high as 70% if they have c-kit mutation. Once relapse, the prognosis is extremely poor. Stem cell transplantation (allo-HSCT) can not improve the prognosis. Therefore, it is possible to improve the prognosis by identifying the high-risk relapse population as early as possible and preemptively treating these patients with means including hematopoietic stem cell transplantation. Cytogenetic changes greatly affect the treatment and prognosis of patients. With the cont...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/118C12Q2600/156C12Q2600/16
Inventor 王秀莉洪燕玄兆伶李大为梁峻彬陈重建
Owner ANNOROAD GENE TECH BEIJING
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