Kit for diagnosing acute myelocytic leukemia
A technology for acute myeloid and leukemia, applied in the field of kits for diagnosing acute myeloid leukemia, can solve problems such as death, and achieve the effects of reducing false positive rate, improving detection sensitivity and specificity
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[0030] Embodiment one: the fluorescent RT-PCR method that diagnoses human AML to occur
[0031] (1) Material:
[0032] Trizol reagent was purchased from Invitrogen Company of the United States, Ologo dT, reverse transcription reaction solution, M-MLV reverse transcriptase, RNase inhibitor, dNTPs, SYBR Green polymerase chain reaction system, and PCR buffer were purchased from Fermentas Company of the United States, ABI 7500 The fluorescence quantitative system is a product of Applied Biosystems, USA.
[0033](2) Primer design and synthesis:
[0034] Using the full-length cDNA sequence as a template, use Prime5 software to analyze the primer sites, and select the best combination based on the consideration of the genomic DNA sequence.
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