Kit for diagnosing acute myelocytic leukemia

Abstract

The invention discloses a kit for diagnosing acute myelocytic leukemia. The kit comprises a reverse transcription system and a PCR (Polymerase Chain Reaction) amplification system, and is characterized in that: the reverse transcription system comprises repetition T oligonucleotide Ologod T, reverse transcription reaction solution, an M-MLV reverse transcriptase, an RNA (Ribonucleic Acid) enzyme inhibitor and dNTPs (Deoxynucleotide Triphosphate), wherein the PCR amplification system comprises an SYBRGreen PCR system and a primer pair; the reverse transcription reaction solution comprises diethyl pyrocarbonate treated water and M-MLV reverse transcriptase buffer solution; the SYBRGreen PCR system comprises PCR buffer solution, template cDNA (complementary Deoxyribonucleic Acid), dNTPs and an SYBRGreen fluorescent dye; and the primer pair is one capable of amplifying human Lc3 synthetase beta3Gn-T5 and human beta-actin. According to the invention, a method for detecting expression of the Lc3 synthetase beta3Gn-T5 by using the SYBRGreen technology is constructed, and the kit capable of conveniently, accurately and sensitively diagnosing the acute myelocytic leukemia is provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a quantitative RT-PCR detection kit, in particular to a real-time fluorescent quantitative RT-PCR detection kit for human glycosyltransferase, Lc3 synthetase β3Gn-T5, which is a kind of reagent kit by reversing It is a kit for accurately and quantitatively detecting the mRNA expression of Lc3 synthetase β3Gn-T5 in the specimen by combining with real-time fluorescent quantitative PCR detection technology to obtain cDNA from the recorded (RT) mRNA sample. Background technique [0002] Tumor is a new organism formed by the body under the action of various carcinogenic factors, a certain cell in the local tissue loses its normal regulation of its growth at the gene level, resulting in abnormal clonal proliferation. It is generally believed that tumor cells are monoclonal, that is, all tumor cells in a tumor are descendants of a mutant cell. Tumors are generally divided into benign and mal...

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Problems solved by technology

[0005] At present, standard chemotherapy can achieve a complete remission (CR) rate of about 70-80%, but most of the patients after CR will eventually relapse, and even some patients are difficult to achieve CR and become refractory leukemia, and the treatment is ineffective and dies

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