Rapid extraction kit for animal tissue genome RNA and extraction method and application

Abstract

The invention relates to a rapid extraction kit for animal tissue genome RNA and an extraction method and application. The rapid extraction kit comprises lysate, flushing liquid 1, flushing liquid 2, absolute ethyl alcohol and a diethyl pyrocarbonate solution; when in extracting, solid animal tissue is milled, and liquid animal tissue is directly extracted; after a sample is cracked, the genome RNA can be absorbed to an RNA absorbing column, and then the diethyl pyrocarbonate solution is used for flushing to obtain the complete genome RNA. The kit is applicable to extraction and purifying of the genome RNA of the animal tissue; the whole extraction costs 5 minutes; the extraction efficiency is high, and the detection sensitivity is high; reliable experience basis is provided to early diagnose various RAN bacteria or viral pathogen infection. The kit is applicable to all research and development institutions, clinical pathogen detection, molecular genetics research, clinical gene diagnosis, animal disease diagnosis and disease control centers, and has a wide market prospect and high economic benefit.

Description

technical field [0001] The invention relates to a kit for rapid extraction of genome RNA from animal tissues, an extraction method and application thereof, and belongs to the field of biotechnology. Background technique [0002] The extraction and purification of RNA in molecular biology research is the first step in molecular experiments, and it is necessary to extract and purify RNA in various clinical tests or scientific research. At present, a variety of kits for detecting RNA based on RT-PCR technology have been used in clinical diagnosis and detection at home and abroad. The RNA extraction method provided by these kits is mainly the phenol-chloroform extraction method. Phenol and chloroform are denaturants in the RNA extraction process, and repeated extractions can remove proteins, salts, and impurities. However, phenol and chloroform are highly toxic to the human body and highly volatile, and the entire extraction process is cumbersome and time-consuming, which often...

AI Technical Summary

Problems solved by technology

Animal tissue contains a large amount of protein, salt and impurities, whose properties are very similar to those of nucleic acid, and it is difficult to separate them from the extracted RNA. In the process of animal tissue RNA extraction, it is easy to co-precipitate with RNA and difficult to separate , often resulting in insufficient purity and concentration of extracted RNA, which affects subsequent amplification and sequencing

[0005] In order to reduce or remove proteins, salts and impurities in animal tissues, the existing RNA extraction process often has pollution, and the steps are cumbersome and the extraction time is long, such as sodium perchlorate method, SDS method and urea method. At the same time, the sample size required by these methods is large, and organic substances such as phenols are easy to remain in the RNA solution, especially in the subsequent RT-PCR amplification process, which will interfere with the amplification process and affect the amplification results. App is restricted

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