31results about How to "Economic gain" patented technology

The invention discloses an experiment device for synthesizing a marine natural gas hydrate sample. The device comprises a reaction kettle, an axial compression piston, a kettle outer jacket, an injection system, an axial compression control system, a confining pressure control system and an output system. The invention further discloses an experiment method adopting the experiment device for synthesizing the marine natural gas hydrate sample. The method comprises the steps that firstly, ice powder particles are manufactured, then, the ice powder particles and dried porous medium particles are mixed and placed into the high-pressure reaction kettle in the subzero low-temperature environment so as to simulate seabed geomechanical properties, then, methane is injected to generate a hydrate, ice is directly converted into the hydrate, and finally free gas inside the reaction kettle is removed through the liquid injection system. According to the experiment device and method, true marine natural gas hydrate samples which are hard to obtain on various geological conditions and various occurrence form conditions can be economically, efficiently and accurately obtained, the research on the hydrate is closer to the reality, and the experiment basis is provided for research of natural gas hydrate exploitation.

The invention relates to a method for cultivating aerobic short-cut nitrification granular sludge for treating domestic sewage, which is characterized in that an SBR reactor adopts ratio of height to diameter of 1.8-10 and volume exchange rate of 50-67%; the sedimentation time is 30-50min, which is gradually shortened to 3min, or the sedimentation time is set as 5-8min. In the process of biochemical reaction, dissolved oxygen (DO) and pH value are regarded as real-time control parameters; aeration time is controlled according to the fuzzy control rule; nitrification is controlled to stop on the short-cut nitrification stage. The method can increase the content of ammonia oxidizing bacteria and volume load, shorten nitration reaction time, improve the sedimentation property of the sludge by a wide margin, shorten sedimentation time, narrow the floor area of the reactor and solve the problems of low operating efficiency and unstable nitrosification caused by time program control of the sewage treatment system. The aerobic short-cut nitrification granular sludge prepared by the invention is suitable for treating high-concentration ammonia nitrogen wastewater with low COD and is a biological wastewater treatment technology with broad market prospect.

The invention provides a harvesting method of microalgae mediated by fungus. The method is characterized by comprising the following steps: (1) transferring microalgae; (2) culturing the microalgae in high density; (3) culturing spores of fungus capable of balling; (4) adding fresh spore suspension into a microalgae culture medium; (5) forming a fungus-mediated large-grained fungus-algae homobium; and (6) harvesting the large-grained bacillus-algae homobium by a filtration method. According to the method, the fungus strain capable of balling is introduced and cultured in a mixing way in the microalgae culture medium to be harvested, so that the aim of symbiosis and balling of fungus and microalgae can be achieved, and the fungus-mediated large-grained fungus-algae homobium can be harvested by virtue of a simple filtration method.

A process for producing isobutylene and methanol comprising: a first step of subjecting methyl-tert-butyl ether to decomposition in the presence of a catalyst to obtain a reaction liquid; a second step of washing the resultant with water to separate the reaction liquid into an oil layer and an aqueous layer; a third step of subjecting the oil layer to distillation to obtain a fraction containing isobutylene and a dialkyl ether from the top of a distillation column, a fraction containing methyl-tert-butyl ether and the heavy components from the bottom of the column and a fraction containing methyl-tert-butyl ether from a side cut of the column, and recycling the side cut fraction to the first step; and a fourth step of subjecting the aqueous layer to distillation to obtain a fraction containing methanol from the top of a distillation column and a fraction containing water from the bottom of the column.

The present invention relates to an antigenic B-cell multi-epitope peptide linked in tandem in the outer membrane protein(Omp) U gene of vibrio mimicus, a making method and an application thereof, which belong to the field of molecular immunology. The B-cell multi-epitope peptide linked in tandem can induce fish to make protective immunity response to vibrio mimicus infection. In the present invention, the amino acid sequence of the antigenic B-cell multi-epitope peptide linked in tandem in the OmpU is shown in SEQIDNO:10. The B-cell multi-epitope peptide linked in tandem in the OmpU of vibrio mimicus is made by a genetic engineering technique. The verification of immune blotting analysis, specific antibody detection and immune animal protective experiments shows that the peptide can elicit efficient and specific protective humoral immunity response to vibrio mimicus infection. The B-cell multi-epitope peptide linked in tandem can be used for the immune diagnosis and the immune prevention and treatment of aquatic animal ascitic diseases. The present invention has high social benefit and economic benefit.

The invention provides a cell model and a screening method for screening a calcium-activated chloride ion channel inhibitor. The screening method sequentially comprises the steps of (1) constructing eukaryotic expression vectors of Anoctamin1 or Anocatmin2 and YFP-H148Q / I152L; (2) stably transfecting the two vectors into FRT (Fisher rat thyroid) cells respectively to ensure that the RFT cells co-express two proteins of YFP-H148Q / I152l and Anoctamin1 or Anocatmin2, and performing multiple times of limiting dilution to obtain an FRT cell model; (3) transferring the FRT cell model to a black-wall microporous plate with a black wall and a transparent bottom; (4) adding a small molecular compound to be detected into the black-wall microporous plate, incubating, then adding a regent for raising the concentration of calcium ions in cells and high-concentration iodide ions, and screening the calcium-activated chloride channel inhibitor by detecting the change of relative fluorescence intensity.

The invention provides a satellite remote sensing monitoring method for the content of chlorophyll a of jujube tree canopy, comprising: determining a to-be-monitored area; downloading the satellite remote sensing image of the to-be-monitored area; performing geometric correction, radiation correction and atmospheric correction to the downloaded satellite remote sensing image; cutting the satellite remote sensing image after atmospheric correction to obtain the image of the monitored area; using ENVI5.1 to calculate the five vegetation indexes for the selected pixels of the monitored area; and substituting the five vegetation indexes into a model through band wave operation to calculate the content of chlorophyll a of jujube tree canopy in each pixel wherein Y represents the content of chlorophyll a of jujube tree canopy and the mass is mg / g. The method provided by the invention realizes the rapid, accurate, economical and environmentally friendly acquisition of the content data of chlorophyll a of the jujube canopy by a regional scale, and avoids the environmental pollution and the damage to the human body in the process of measuring the exhausted chemical liquid, greatly simplifying the operation steps and shortening the monitoring time.

The invention provides a satellite remote sensing monitoring method for the jujube tree canopy chlorophyll b content. The method comprises the following steps that a to-be-monitored area is determined; a satellite remote sensing image of the to-be-monitored area is downloaded; geometric precision correction, radiation correction and atmospheric correction are sequentially conducted on the downloaded satellite remote sensing image; the satellite remote sensing image obtained after atmospheric correction is cropped to obtain a monitored area image; five vegetation indexes of each pixel selected from the monitored area are calculated through ENVI5.1 software; the five vegetation indexes are substituted into a model through waveband calculation, and the jujube tree canopy carotenoid content of each pixel is calculated, wherein Y is the jujube tree canopy chlorophyll b content, and the unit of the jujube tree canopy chlorophyll b content is mg / g. According to the method, the jujube tree canopy chlorophyll b content data is quickly and accurately acquired in an economical and environment-friendly mode on regional scale, pollution to the environment and harm to human bodies of chemical tail liquid discharge in the measurement process are avoided, the operation steps are greatly simplified, and the monitoring time is shortened.

The invention discloses a method for estimating a power factor of an induction motor in any load state, and relates to the technical field of motor parameter estimation. For the power factor of the three-phase induction motor under any load, the method comprises the steps: acquiring the data of the motor under different load states, obtaining a data set of the load rate and the power factor through calculation, obtaining a regression model for estimating the power factor through training after the data set is divided, performing verification, obtaining a final model through a lowest generalization error, and then carrying out the test verification. The power factor of the motor in any load state is accurately calculated through the power factor estimation model, and the adjustment of the induction motor capacitor bank is guided. The method is high in accuracy, and the power factor can be rapidly, economically and continuously obtained.

The invention provides a satellite remote sensing monitoring method for the content of jujube tree canopy carotenoid. The method comprises the following steps of determining a region to be monitored; downloading a satellite remote sensing image of the region to be monitored; sequentially performing geometric precise correction, radiation correction and atmospheric correction on the downloaded satellite remote sensing image; cutting the satellite remote sensing image obtained after atmospheric correction and obtaining an image of the monitored region; calculating the monitored region through ENVI5.1 software for selecting seven vegetation indexes of pixels; substituting the seven vegetation indexes into a model through wave band calculation, and working out the content of the jujube tree canopy carotenoid of each pixel, wherein Y is the content of the jujube tree canopy carotenoid, and the unit is mg / g. According to the method, the content data of the jujube tree canopy carotenoid is rapidly and accurately obtained in the regional scale in an economical and environment-friendly mode, environmental pollution and human injuries caused by chemical tail liquid discharge in the determination process are avoided, the operation steps are greatly simplified, and the monitoring time is shortened.

The invention discloses a method by using pSilencer plasmid to quickly establish a siRNA carrier. The invention comprises the steps of: the establishment of a sub-cloning carrier, a siRNA synthesis method used for quick screening course and quick screening methods of positive clone with siRNA, etc. Compared with traditional carrier establishing method, the method of the invention can more effectively, quickly and economically obtain required small reference gene carrier and can be more particularly applicable for function verification and analysis for large-scaled primary screening results of genes such as gene chip, SSH and SAGE, etc. and research of RNA reference mechanism for seeking effective inhibiting sequence siRNA, etc.

Provided are a method for preparing a highly concentrated fibrinogen solution, and a method for preparing a fibrin sealant component 1 containing the highly concentrated fibrinogen solution and Factor ChiIII. More particularly, the method for preparing a highly concentrated fibrinogen solution according to the present invention can be prepared by adding amino acid or amino acid derivatives, and / or salts, etc. to a lowly concentrated fibrinogen solution separated from blood plasma and highly concentrating fibrinogen using an ultra filtration method, and a fibrin sealant component 1 in order to be used in a fibrin sealant product can be prepared by adding Factor ChiIII before or after the ultra filtration in the method for preparing the highly concentrated fibrinogen. The fibrin sealant component 1 prepared by the method according to the present invention may be cryopreserved in a liquid state, be preserved for a long time at room temperature, and be immediately applied to treatment without performing a reconstitution process. In addition, the present invention relates to a fibrin sealant product configured of the fibrin sealant component 1 and a fibrin sealant component 2 which is a solution containing thrombin and calcium chloride filled in a separate container. The fibrin sealant product according to the present invention may be provided in a vial type in which the fibrin sealant component 1 and the fibrin sealant component 2 are filled in separate vials, respectively, or a pre-filled type in which the fibrin sealant component 1 and the fibrin sealant component 2 are filled in separate syringes connected with each other to thereby be immediately used.

The invention discloses a dark sun green glass. The dark sun green glass comprises a glass basic component and a glass coloring component, the thickness of the prepared dark sun green glass is 1.6-2.1 mm, the solar energy transmittance is less than or equal to 65%, the infrared ray transmittance is less than or equal to 45%, and the ultraviolet ray transmittance is less than or equal to 35%. According to the invention, the thickness of the glass is reduced, and the solar energy transmittance, infrared ray transmittance and ultraviolet ray transmittance of the glass can be reduced at the same time.

The invention provides a small molecule compound high-throughput screening method for treating optic neuromyelitis and provides a small molecule compound used for treating the optic neuromyelitis andcapable of blocking a series of inflammation reaction induced by binding of NMO-IgG to AQP4 on the surfaces of astrocyte membranes. The method is scientific, reasonable and high in adaptability. The small molecule compound is safe, reliable and good in treatment effect. The chemical structural formulas are respectively shown as follows.

The invention provides a harvesting method of microalgae mediated by fungus. The method is characterized by comprising the following steps: (1) transferring microalgae; (2) culturing the microalgae in high density; (3) culturing spores of fungus capable of balling; (4) adding fresh spore suspension into a microalgae culture medium; (5) forming a fungus-mediated large-grained fungus-algae homobium; and (6) harvesting the large-grained bacillus-algae homobium by a filtration method. According to the method, the fungus strain capable of balling is introduced and cultured in a mixing way in the microalgae culture medium to be harvested, so that the aim of symbiosis and balling of fungus and microalgae can be achieved, and the fungus-mediated large-grained fungus-algae homobium can be harvested by virtue of a simple filtration method.

The invention discloses a corn damage degree discrimination method based on a remote sensing technology, and the method comprises the steps: carrying out the image preprocessing of a medium-resolution satellite image, obtaining multispectral remote sensing data, obtaining an image of a normalized difference vegetation index (NDVI) and an image of a red edge position index (REP), carrying out the grading of the loss rate of corn, constructing a two-dimensional loss rate estimation matrix, and carrying out the recognition of the damage degree of corn. And estimating the loss rate of the corn according to the two-dimensional loss rate estimation matrix to obtain the drought disaster loss degree of the insurance corn. According to the method, the corn drought damage degree is evaluated by using a remote sensing technology, so that the occurrence range and the damage degree of the corn drought disaster can be efficiently, quickly, accurately and economically obtained in a short time under the condition that manpower, material resources and financial resources are limited, and a support basis is provided for nuclear protection loss assessment of the corn drought disaster.

The invention discloses a method for rapidly constructing an siRNA carrier by using pSilencer plasmid. The steps include the construction of subcloning vector, the synthesis method of siRNA used in the rapid screening process, the rapid screening method of positive clones containing siRNA, and the like. Compared with conventional vector construction methods, this method is more effective, faster, and more economical to obtain the required small-scale gene vectors, and is especially suitable for functional verification analysis of large-scale gene screening results such as gene chips, SSH, and SAGE. , and research on the search for effective inhibitory sequence siRNA and other RNA interference mechanisms.

The invention relates to an anaerobic fermentation method, a fermentation product and an animal feed comprising the fermentation product. During anaerobic fermentation, raw materials are improved biotechnologically, and in particular, a feeding value of plant dregs is increased. The anaerobic fermentation method includes the step of mixing the plant dregs with a hydrocarbon compound and an enzyme. By the anaerobic fermentation method, fermentation time in industrial production is shortened effectively and product nutrition value is enhanced.

The invention provides a satellite remote sensing monitoring method for the jujube tree canopy chlorophyll b content. The method comprises the following steps that a to-be-monitored area is determined; a satellite remote sensing image of the to-be-monitored area is downloaded; geometric precision correction, radiation correction and atmospheric correction are sequentially conducted on the downloaded satellite remote sensing image; the satellite remote sensing image obtained after atmospheric correction is cropped to obtain a monitored area image; five vegetation indexes of each pixel selected from the monitored area are calculated through ENVI5.1 software; the five vegetation indexes are substituted into a model through waveband calculation, and the jujube tree canopy carotenoid content of each pixel is calculated, wherein Y is the jujube tree canopy chlorophyll b content, and the unit of the jujube tree canopy chlorophyll b content is mg / g. According to the method, the jujube tree canopy chlorophyll b content data is quickly and accurately acquired in an economical and environment-friendly mode on regional scale, pollution to the environment and harm to human bodies of chemical tail liquid discharge in the measurement process are avoided, the operation steps are greatly simplified, and the monitoring time is shortened.

The invention provides a cell model and a screening method for screening a calcium-activated chloride ion channel inhibitor. The screening method sequentially comprises the steps of (1) constructing eukaryotic expression vectors of Anoctamin1 or Anocatmin2 and YFP-H148Q / I152L; (2) stably transfecting the two vectors into FRT (Fisher rat thyroid) cells respectively to ensure that the RFT cells co-express two proteins of YFP-H148Q / I152l and Anoctamin1 or Anocatmin2, and performing multiple times of limiting dilution to obtain an FRT cell model; (3) transferring the FRT cell model to a black-wall microporous plate with a black wall and a transparent bottom; (4) adding a small molecular compound to be detected into the black-wall microporous plate, incubating, then adding a regent for raising the concentration of calcium ions in cells and high-concentration iodide ions, and screening the calcium-activated chloride channel inhibitor by detecting the change of relative fluorescence intensity.

The invention discloses an experimental device for synthesizing marine natural gas hydrate samples, which comprises a reaction kettle, an axial pressure piston, an outer jacket of the kettle, an injection system, an axial pressure control system, a confining pressure control system and an output system. The invention also discloses an experimental method for preparing marine natural gas hydrate samples by using the above-mentioned experimental device. Firstly, ice powder particles are made, and then the ice powder particles are mixed with dried porous medium particles, and placed in a low temperature environment below zero. In the high-pressure reactor to simulate the geomechanical properties of the seabed, methane gas is injected to generate hydrate, and the ice is directly converted into hydrate. Finally, the free gas in the reactor is driven out through the liquid injection system. The present invention can economically, effectively and accurately obtain real marine natural gas hydrate samples under various geological conditions and various occurrence conditions that are difficult to obtain, making hydrate research closer to reality, and providing research on natural gas hydrate exploitation. Experimental basis.

The invention provides a satellite remote sensing monitoring method for the total chlorophyll content of the canopies of jujube trees. The satellite remote sensing monitoring method for the total chlorophyll content of the canopies of the jujube trees includes the following steps: determining a region to be monitored; downloading a satellite remote sensing image of the region to be monitored; successively carrying out geometric precision correction, radiation correction and atmospheric correction on the downloaded satellite remote sensing image; cutting the satellite remote sensing image after the satellite remote sensing image is subjected to atmospheric correction to obtain an image of a monitored region; calculating 6 vegetation indexes of selected picture elements of the monitored region by software ENVI 5.1; substituting the 6 vegetation indexes into a model through band calculation, and calculating the total chlorophyll content of the canopies of the jujube trees in each picture element, wherein Y is the total chlorophyll content of the canopies of the jujube trees, and the unit of the total chlorophyll content is mg / g. By the method, the total chlorophyll content data of the canopies of the jujube trees can be rapidly, accurately and economically obtained in an environment-friendly manner in regional scale, environmental pollution and injury to human bodies due to the discharge of chemical tail liquid in a measuring process are avoided, operation steps are greatly simplified, and monitoring time is shortened.

The invention provides a satellite remote sensing monitoring method for jujube canopy chlorophyll a content, comprising the following steps: determining the area to be monitored; downloading the satellite remote sensing image of the area to be monitored; performing geometric precision correction and radiation correction on the downloaded satellite remote sensing image in sequence , Atmospheric correction; crop the satellite remote sensing image after atmospheric correction to obtain the monitoring area image; use ENVI5.1 software to calculate the 5 vegetation indices of the selected pixels in the monitoring area; substitute the 5 vegetation indices into the model through band operation, and calculate The chlorophyll-a content of jujube canopy in each pixel; where, Y is the chlorophyll-a content of jujube canopy in mg / g. The method provided by the invention realizes rapid, accurate, economical and environmentally friendly acquisition of jujube canopy chlorophyll a content data on a regional scale, avoids environmental pollution and human body damage caused by chemical tail liquid discharge during the measurement process, and greatly simplifies the operation steps , to shorten the monitoring time.

The present invention relates to an antigenic B-cell multi-epitope peptide linked in tandem in the outer membrane protein(Omp) U gene of vibrio mimicus, a making method and an application thereof, which belong to the field of molecular immunology. The B-cell multi-epitope peptide linked in tandem can induce fish to make protective immunity response to vibrio mimicus infection. In the present invention, the amino acid sequence of the antigenic B-cell multi-epitope peptide linked in tandem in the OmpU is shown in SEQIDNO:10. The B-cell multi-epitope peptide linked in tandem in the OmpU of vibrio mimicus is made by a genetic engineering technique. The verification of immune blotting analysis, specific antibody detection and immune animal protective experiments shows that the peptide can elicit efficient and specific protective humoral immunity response to vibrio mimicus infection. The B-cell multi-epitope peptide linked in tandem can be used for the immune diagnosis and the immune prevention and treatment of aquatic animal ascitic diseases. The present invention has high social benefit and economic benefit.

The invention relates to an anaerobic fermentation method, a fermentation product and an animal feed comprising the fermentation product. During anaerobic fermentation, raw materials are improved biotechnologically, and in particular, a feeding value of plant dregs is increased. The anaerobic fermentation method includes the step of mixing the plant dregs with a hydrocarbon compound and an enzyme. By the anaerobic fermentation method, fermentation time in industrial production is shortened effectively and product nutrition value is enhanced.

The present invention provides a method for artificially cultivating seawater leech by using Echinococcus chinensis, that is, using Echinococcus chinensis as host fish, and establishing an artificial culture and passage system for leech, which can stably and massively obtain seawater leeches, thereby Make up for the deficiencies of the existing technology. The present invention establishes a method for artificially cultivating seawater leech Z. arugamensis by using Echinococcus damselfish, which can economically and conveniently obtain leech egg cocoons of different developmental stages, as well as a large number of young leeches and adult leeches anytime and anywhere. Thereby breaking through the bottleneck of seawater leech breeding, and providing important biological materials for the pathogen, pathology, pathogenic mechanism, detection and prevention of leech disease.

The prior art discloses edge banding composed of mixed materials: metal and wood veneer. A process for production of a profile strip, in particular edge strip, is provided for low-cost production of a profile strip, in particular edge strip, with different decorative elements, which in particular is easier to process and has higher chemical resistance and moisture resistance, and also scratch resistance, the steps in the process being as follows: production of a plastics profile in the form of a core strip (1), application of a decorative base material (5) to an upper side of the core strip, application of a decorative finish (7) to the decorative base material, in such a way that the decorative finish hides sections of the decorative base material and forms, with the decorative base material, at least a portion of the decorative effect of the profile strip, where the decorative base material (5) applied comprises a foil.

The invention relates to a method and a device for obtaining liquid nitrogen by utilization of the cooling capacity of liquefied natural gas. The method at least comprises the following steps: raw material nitrogen and the liquefied natural gas perform heat exchange in a natural gas heat exchanger; and the raw material nitrogen which is led out of an air separation unit or an external nitrogen pipe is compressed by a nitrogen turbocompressor, enters into a nitrogen channel of the natural gas heat exchanger for heat exchange, and is cooled by returned cold flow. The device consists of a low-temperature nitrogen circulator, the nitrogen turbocompressor, the natural gas heat exchanger, a high-temperature booster expansion turbine, a low-temperature booster expansion turbine, a gas-liquid separator and a liquid nitrogen subcooler. The method and the device fully utilize the high-quality cold energy of the liquefied natural gas, and can economically obtain liquid nitrogen; and the power consumption of the device in producing 1 kilogram of the liquid nitrogen is only 0.24 kilowatt per hour while the power consumption of the prior air separation unit in producing 1 kilogram of the liquid nitrogen is between 0.6 and 0.8 kilowatt per hour, so that the device has the characteristics of obvious energy-saving effect and so on.

The invention relates to a method for cultivating aerobic short-cut nitrification granular sludge for treating domestic sewage, which is characterized in that an SBR reactor adopts ratio of height to diameter of 1.8-10 and volume exchange rate of 50-67%; the sedimentation time is 30-50min, which is gradually shortened to 3min, or the sedimentation time is set as 5-8min. In the process of biochemical reaction, dissolved oxygen (DO) and pH value are regarded as real-time control parameters; aeration time is controlled according to the fuzzy control rule; nitrification is controlled to stop on the short-cut nitrification stage. The method can increase the content of ammonia oxidizing bacteria and volume load, shorten nitration reaction time, improve the sedimentation property of the sludge by a wide margin, shorten sedimentation time, narrow the floor area of the reactor and solve the problems of low operating efficiency and unstable nitrosification caused by time program control of the sewage treatment system. The aerobic short-cut nitrification granular sludge prepared by the invention is suitable for treating high-concentration ammonia nitrogen wastewater with low COD and is a biological wastewater treatment technology with broad market prospect.

The invention belongs to the technical field of satellite remote sensing monitoring, and provides a satellite remote sensing monitoring method for the chlorophyll content of a cotton canopy. The method comprises the following steps: determining a monitoring area, acquiring a satellite remote sensing image of the monitoring area, correcting the acquired satellite remote sensing image and cutting the corrected satellite remote sensing image, and substituting the obtained five vegetation indexes of TGI, GLI, IO, RGRI and VARI and the reflectivity data of the four wave bands of SWIR2, NIR, Rb andCoast into a model through wave band operation, thereby obtaining the green content data of the cotton canopy leaves through calculation. The method has the advantages that the chlorophyll content ofthe large-area cotton field can be monitored more quickly and economically in a labor-saving manner, the requirement for quickly acquiring canopy chlorophyll content data of the cotton field of a regional scale in agricultural production can be met, and a basis is provided for accurate management of cotton field management.