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Harvesting method of microalgae mediated by fungus

A harvesting method and microalgae technology, applied in the field of renewable bio-energy, can solve the problems of high cost, high energy consumption, unsuitable for large-scale production applications, etc., and achieve the effect of controlling costs

Inactive Publication Date: 2012-03-28
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional microalgae harvesting methods mainly include air flotation, centrifugation, adding flocculant sedimentation, membrane filtration, etc. However, these methods either consume too much energy, cost too much, or are not suitable for large-scale production applications

Method used

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  • Harvesting method of microalgae mediated by fungus
  • Harvesting method of microalgae mediated by fungus
  • Harvesting method of microalgae mediated by fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Oleaginous algal strains—Chlorella ( Cholorella vulgaris ) for local screening, and its autotrophic medium formula is as follows.

[0041] The formula of the above-mentioned autotrophic medium is: K 2 HPO 4 ·3H 2 O 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L, CaCl 2 2H 2 O 0.036g / L, citric acid 0.006g / L, ferric ammonium citrate 0.006g / L, EDTA 0.001g / L, NaNO 3 1.5g / L, Na 2 CO 3 0.02g / L, A5 trace element solution 1.5ml / L, of which A5 trace element solution composition: H 3 BO 3 2.86g / L, MnCl 2 4H 2 O 1.81g / L, ZnSO 4 ·7H 2 O 0.222g / L, NaMoO 4 2H 2 O 0.39g / L, CuSO 4 ·5H 2 O 0.079g / L, and CoCl 2 ·6H 2 O 0.05g / L.

[0042] The formula of the heterotrophic medium is the same as above, and the organic carbon source is added to the initial reducing sugar concentration of 20 g.L -1 .

[0043] By the method described in step 1, inoculate the strong individual colony of Chlorella grown on solid medium into a 250 mL shaker flask for shaking culture, and the temperatu...

Embodiment 2

[0047] Oil-bearing algal strains—Scenedes (Scenedesmus sp.) For local screening, the formula of the autotrophic medium is as follows.

[0048] The formula of the above-mentioned autotrophic medium is: K 2 HPO 4 ·3H 2 O 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L, CaCl 2 2H 2 O 0.036g / L, citric acid 0.006g / L, ferric ammonium citrate 0.006g / L, EDTA 0.001g / L, NaNO 3 1.5g / L, Na 2 CO 3 0.02g / L, A5 trace element solution 1.5ml / L, of which A5 trace element solution composition: H 3 BO 3 2.86g / L, MnCl 2 4H 2 O 1.81g / L, ZnSO 4 ·7H 2 O 0.222g / L, NaMoO 4 2H 2 O 0.39g / L, CuSO 4 ·5H 2 O 0.079g / L, and CoCl 2 ·6H 2 O 0.05g / L.

[0049] The formula of the heterotrophic medium is the same as above, and the organic carbon source is added to the initial reducing sugar concentration of 22 g.L -1 .

[0050] By the method described in step 1, inoculate the strong individual colony of Chlorella grown on solid medium into a 250 mL shaker flask for shaking culture, and the temperature ...

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Abstract

The invention provides a harvesting method of microalgae mediated by fungus. The method is characterized by comprising the following steps: (1) transferring microalgae; (2) culturing the microalgae in high density; (3) culturing spores of fungus capable of balling; (4) adding fresh spore suspension into a microalgae culture medium; (5) forming a fungus-mediated large-grained fungus-algae homobium; and (6) harvesting the large-grained bacillus-algae homobium by a filtration method. According to the method, the fungus strain capable of balling is introduced and cultured in a mixing way in the microalgae culture medium to be harvested, so that the aim of symbiosis and balling of fungus and microalgae can be achieved, and the fungus-mediated large-grained fungus-algae homobium can be harvested by virtue of a simple filtration method.

Description

technical field [0001] The invention belongs to the field of renewable bioenergy, and in particular relates to a new process for fungus-mediated microalgae harvesting. Specifically, the microalgae cells are first allowed to grow under photoautotrophic or heterotrophic conditions; then fungal spores that can form balls are introduced at different stages of cell growth, and the culture conditions are controlled to finally form large-grained fungal spheroids, and then use A process in which microalgae are harvested by simple filtration methods and used in bioenergy production. Background technique [0002] Microalgae are microorganisms capable of photosynthesis. It can use small molecule organic matter and inorganic nutrients in sewage to absorb a large amount of CO in the air 2 , and synthesize fat, which can be refined into biodiesel through collection and processing, which can be used as power fuel for automobiles and trains, and can also be used as aviation fuel for aircr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89C12R1/845C12R1/82C12R1/785C12R1/66C12R1/885C12R1/645
Inventor 阮榕生周文广程艳玲李筠刘玉环林向阳
Owner NANCHANG UNIV
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