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A method of artificially cultivating seawater leeches using the damselfish

A technology for damselfish and seawater fish, applied in the field of aquaculture, can solve the problems of difficulty in obtaining parasite samples for research work, and less research on leeches

Active Publication Date: 2021-10-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because fish parasites (including leeches) are not as easy to cultivate and reproduce artificially as fish bacteria and viruses, it is difficult to obtain sufficient parasite samples for research except for collecting a small amount of parasites on the surface of diseased fish work use
A large number of artificial breeding of leeches has become a bottleneck in related research work, resulting in less research on Z. arugamensis at home and abroad

Method used

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  • A method of artificially cultivating seawater leeches using the damselfish
  • A method of artificially cultivating seawater leeches using the damselfish
  • A method of artificially cultivating seawater leeches using the damselfish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Hatching eggs and production of young places

[0028] 1) Pick the seeds

[0029] Choose a body length in 10mm or more, and 16 calendar Z.arugamensis in the body of the body.

[0030] 2) Production of eggs

[0031] Put the selected seeds into a 12-well cell culture plate, one of the apertures containing 2 ml of salinity 30 per well, cultured in a constant temperature incubator at 26 ° C, 1 / 2 sea water per 2D . After 4 days, the seed was removed and the eggs were observed. During this time, 16 species of the vermicaphysics were 259.

[0032] 3) Hatching eggs

[0033] Eggs continue to incubate in a constant temperature incubator at 26 ° C, transfer 1 / 2 seawater every 2D. After 10 days, 259 eggs were incubated with 217 young blood, and the hatching rate was 83.78%.

[0034] 4) Observing the development of eggs

[0035] 20 eggs were randomly selected, and the development of eggs was observed under an inverted microscope. The fish is yellowish brown, the shape is simil...

Embodiment 2

[0039] Example 2: Cultivated Yangpeng

[0040] 1) Roasted ribbon

[0041] Choosing a 6-tid of a healthy bitbarsel marties, cultured in 3 2L glass beakers, and 2 per capitle 2. During the aquaculture, water temperature is 26 ° C, salinity 30. For 1 / 3 sea water every day, feed it once.

[0042] 2) Summer's development

[0043] The incubated 30 young blood was transferred to the above-mentioned beaker containing the chepackwarm, 10 piths per beaker. The young vermischers will find the host fish in their own, and they are attached to the fingers of the host fish, the eyes, etc. image 3 ). After 1D, the blood body of the sustaining the host fish was black, the body length was about 2.2mm, and the body became thick. image 3 a). Located with a speed of about 1 mm per day, the body is uniformly uniform, slowly becomes an angry, which can distinguish a narrow neck and a width torso portion. After about 11 days, 30 fish vermor vented length reached 10 ~ 15mm, developed as an enrichment ( Fi...

Embodiment 3

[0045] Example 3: Artificial cultivation of sea waterfish Z.arugamensis using the rattlefish 鲷 人

[0046]Under the conditions of water temperature 26 ° C, salinity 30, use the rattle of cheek owners to cultivate sea waterfish z.arugamensis, fish filming from eggs, it takes 9 days, and the maternal gelling is an enrichment, which is about 20D can complete the cultivation of a generation of fluts.

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Abstract

The present invention provides a method for artificially cultivating seawater leech by using Echinococcus chinensis, that is, using Echinococcus chinensis as host fish, and establishing an artificial culture and passage system for leech, which can stably and massively obtain seawater leeches, thereby Make up for the deficiencies of the existing technology. The present invention establishes a method for artificially cultivating seawater leech Z. arugamensis by using Echinococcus damselfish, which can economically and conveniently obtain leech egg cocoons of different developmental stages, as well as a large number of young leeches and adult leeches anytime and anywhere. Thereby breaking through the bottleneck of seawater leech breeding, and providing important biological materials for the pathogen, pathology, pathogenic mechanism, detection and prevention of leech disease.

Description

Technical field [0001] The invention belongs to the technical field of aquaculture, and in particular to a method of using the ratcanicobdella arugamensis. Background technique [0002] Seawater Film Z.arugamensis is an important in vitro parasite in seawater fish culture. In terms of parasite classification, the fish fillet Z.arugamensis is affiliated with annelida, oligochate, hirudinida, piscicolidae, Zeylanicobdella. [0003] Sea Water Swisper Z.arugamensis Mainly parasitic pectoral fins, straw fins and bases of sea fish, followed by tail fins, hip fins, dorsal fins and eyes. When parasitic, the fish is thin, malnourished, and the body surface produces trauma, which in turn causes secondary infection. Fish fillet Z.arugamensis can also serve as a medium of bacteria, viruses and other parasites, can cause fish death during severe. [0004] Fish fillet Z.arugamensis is mainly popular in Southeast Asia. In recent years, there have been frequently occurred in my country's seawate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A01K61/13
CPCA01K61/00A01K61/13Y02A40/81
Inventor 史成银任宁欣万晓媛谢国驷王仁宝李晨刘莉
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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