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Detection kit for acute hemorrhagic conjunctivitis and detection method thereof

A detection kit and acute hemorrhage technology, applied in the field of detection kits for Enterovirus 70 and Coxsackie A24 virus variants, can solve the problems of economic and medical resource loss, large number of infected people, etc., and avoid false positives. Positive and environmental contamination, less time consuming, highly specific effect

Active Publication Date: 2012-10-03
DAAN GENE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the highly contagious nature of the disease, a large number of people were infected after the outbreak, which brought huge losses to the economy and medical resources

Method used

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  • Detection kit for acute hemorrhagic conjunctivitis and detection method thereof
  • Detection kit for acute hemorrhagic conjunctivitis and detection method thereof
  • Detection kit for acute hemorrhagic conjunctivitis and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Acute hemorrhagic conjunctivitis detection kit and its use

[0050] 1. Prepare a kit comprising the following components:

[0051] Primer-probe mixture (25 μl / tube) 1 tube: the sequences of the forward and reverse primers for EV70 target polynucleotide amplification are 5'-CAACAAGTACCTCATCCATTCAAAAG-3'(SEQ ID NO: 1) and 5'-GTAAACAATTCCATCTTCCTCCT, respectively -3' (SEQ ID NO: 2), EV70 oligonucleotide probe, its sequence is 5'-TTCTTCATCTGGACTTTGAACACCAGAGAACT-3' (SEQ ID NO: 3), the two ends of the probe are respectively combined with the fluorescent group FAM and fluorescence quencher BHQ1; the sequences of the forward and reverse primers used for CA24v target polynucleotide amplification are 5'-GAGTGCTTGCCCAGATTTTAG-3' (SEQ ID NO: 4) and 5'-ATAACGGTGTCAATGGTCTCCTC-3', respectively (SEQ ID NO: 5), the CA24v oligonucleotide probe used for fluorescent PCR amplification and detection system, its sequence is 5'-CGTGCGTCTGTTGAGAGACACCAAC-3' (SEQ ID NO: 6), the two ends of th...

Embodiment 2

[0070] Detection of clinical samples with acute hemorrhagic conjunctivitis detection kit

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Abstract

The invention relates to a detection kit for an enterovirus 70-type (EV70) and Coxackie A24-type virus variants (CA24v) which are relevant to acute hemorrhagic conjunctivitis and a detection method thereof. The kit comprises a RT-PCR (reverse transcription-polymerase chain reaction) reaction buffer solution, enterovirus 70-type (EV70) and Coxackie A24-type virus variant (CA24v) specific forward and reverse primer and fluorescent probe mixed liquor necessary for PCR amplification, a RT-PCR reaction enzyme system, DEPC H2O (diethyl pyrocarbonate) and EV70 and CA24v positive controls as well as negative controls. The invention has stable and reliable result and simple and rapid operation and can rapidly detect the acute hemorrhagic conjunctivitis caused by the enterovirus 70-type and Coxackie A24-type virus variants, and thereby, the early anti-epidemic work can be completed.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a detection kit for enterovirus 70 (EV70) and coxsackie A24 virus variant (CA24v) related to acute hemorrhagic conjunctivitis and a detection method thereof. Background technique [0002] Acute hemorrhagic conjunctivitis, commonly known as pink eye, is one of the Class C infectious diseases reported in the "Law of the People's Republic of China on the Prevention and Control of Infectious Diseases". The disease has a high incidence rate, spreads rapidly, affects a wide range, is highly contagious, and has a short incubation period. The disease occurs in both eyes within 12 to 48 hours after contact with the source of infection, which has a great impact on people's production and life. The crowd is generally susceptible, and the number of adults is more than that of children. Outbreaks and epidemics of acute hemorrhagic conjunctivitis have been reported in man...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
Inventor 方筠田桢干章琪张琳简大钊王健曹敏何宇平何琼董瑞华高秀洁陈华云
Owner DAAN GENE CO LTD
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