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Method for extracting oestrone from euphausia superba and efficient thin layer chromatography scanning and detecting method

A technology of scanning detection and thin-layer chromatography, which is applied in the field of estrone extraction, can solve the problems of environmental pollution, high cost, and long time consumption, and achieve the effects of strong sensitivity, good environment, and high degree of automation

Inactive Publication Date: 2014-05-21
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The detection of estrone is mostly carried out by gas chromatography, liquid chromatography, radioimmunoassay, colorimetry and other methods, but these detection methods have the following shortcomings: the detection process is time-consuming, expensive, and may cause environmental pollution Wait

Method used

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  • Method for extracting oestrone from euphausia superba and efficient thin layer chromatography scanning and detecting method
  • Method for extracting oestrone from euphausia superba and efficient thin layer chromatography scanning and detecting method

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Take 1 g of dried Antarctic krill, add 9 mL of methanol, heat and extract by ultrasonic heating in a 35°C, 350w, 40kHz ultrasonic cleaning machine, extract for 10 minutes, filter the extract, and evaporate the filtrate to dryness in a rotary evaporator to obtain estrone crude extract.

[0047] Dissolve the crude estrone extract obtained above in 5 mL of methanol to make a sample solution; accurately weigh 1.0000 mg of estrone standard (J&K Company) and dissolve it in 1.0 mL of methanol to obtain a concentration of 1 mg mL -1 standard solution.

[0048] Take 5 μL of sample solution and standard solution and spot on the same piece of GF 254 High-efficiency thin-layer silica gel plate (10×10cm 2 ), the developer volume ratio is carbon tetrachloride: ethyl acetate: acetone: glacial acetic acid: toluene: cyclohexane=78-80:9-11:9-11:2-4:3d:2d, will develop The agent is added to the expansion cylinder (10×12×15cm 3 ), pre-equilibrate at room temperature for 35 minutes, the...

Embodiment 2

[0050]Take 1g of dried Antarctic krill, add methanol 11mL, heat and extract by ultrasonic heating in a 35°C, 350w, 40kHz ultrasonic cleaner, extract for 15 minutes, filter the extract, and evaporate the filtrate to dryness in a rotary evaporator to obtain estrone of crude extract.

[0051] Dissolve the crude estrone extract obtained above in 5 mL of methanol to make a sample solution, accurately weigh 1.0000 mg of estrone standard (J&K company) and dissolve it in 1.0 mL of methanol to obtain a concentration of 1 mg mL -1 standard solution.

[0052] Take 5 μL of sample solution and standard solution and spot on the same piece of GF 254 High-efficiency thin-layer silica gel plate (10×10cm 2 ), the developer volume ratio is carbon tetrachloride: ethyl acetate: acetone: glacial acetic acid: toluene: cyclohexane=78-80:9-11:9-11:2-4:3d:2d, will develop The agent is added to the expansion cylinder (10×12×15cm 3 ), pre-equilibrate at room temperature for 40 minutes, then put the h...

Embodiment 3

[0054] Take 1g of dried Antarctic krill, add methanol 10mL, heat and extract by ultrasonic heating in a 35°C, 350w, 40kHz ultrasonic cleaning machine, extract for 20 minutes, filter the extract, and evaporate the filtrate to dryness in a rotary evaporator to obtain estrone of crude extract.

[0055] Dissolve the crude estrone extract obtained above in 5 mL of methanol to make a sample solution; accurately weigh 1.0000 mg of estrone standard (J&K Company) and dissolve it in 1.0 mL of methanol to obtain a concentration of 1 mg mL -1 standard solution.

[0056] Take 5 μL of sample solution and standard solution and spot on the same piece of GF 254 High-efficiency thin-layer silica gel plate (10×10cm 2 ), the developer volume ratio is carbon tetrachloride: ethyl acetate: acetone: glacial acetic acid: toluene: cyclohexane=78-80:9-11:9-11:2-4:3d:2d, will develop The agent is added to the expansion cylinder (10×12×15cm 3 ), pre-equilibrate at room temperature for 45 minutes, then...

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Abstract

The invention discloses a method for extracting oestrone from a euphausia superba and an efficient thin layer chromatography scanning and detecting method. The method for extracting the oestrone from the euphausia superba comprises the following steps: extracting the euphausia superba as a raw material through methanol; and filtering, and steaming a filtrate to dry through a rotary evaporation instrument, so as to obtain a crude extract containing the oestrone. The oestrone of the euphausia superba is detected by using an efficient thin layer chromatography scanning method. A developing solvent is a mixture with a volume ratio of carbon tetrachloride to ethyl acetate to acetone to glacial acetic acid to methylbenzene to cyclohexane of (78-80) to (9-11) to (9-11) to (2-4) to 3d to 2d; a coloring agent is a 5% ferric chloride water solution; the content of the acetone can be obtained through calculation of absorbances of a detection sample product and a standard product by carrying out scanning detection with an absorption wavelength of 600nm by using a CAMAG thin layer chromatography scanner III. The method has the advantages of being simple to operate, fast, accurate and reliable.

Description

technical field [0001] The invention relates to a method for extracting estrone from Antarctic krill and a high-efficiency thin-layer chromatography scanning detection method. Background technique [0002] Estrone [1,3,5(10)-estratrien-3-ol-17-one or 3-hydroxy-1,3,5(10)-estratrien-17-one] or E1 is a molecular weight of C18 steroid hormone of 270.37 daltons with the molecular formula C 18 h 22 o 2 , is a kind of estrogen, and its physiological effect is the physiological effect of estrogen. [0003] Antarctic krill (Euphausia superba) is a kind of krill that lives in the waters of Antarctica. It lives in clusters, sometimes with a density of 10,000-30,000 per cubic meter. They feed on tiny phytoplankton, are about 6cm in length, weigh about 2g, and live up to 6 years. They are the keystone species of the Antarctic ecosystem, and in terms of biomass, they are probably the most successful biological species on Earth. Because of the huge reserves of Antarctic krill and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N1/28
Inventor 马善利刘代成
Owner SHANDONG NORMAL UNIV
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