Bovine tuberculosis antigen specific gamma-interferon enzyme-linked immuno sorbent assay (ELISA) kit

A tuberculosis antigen and gamma interferon technology, applied in the biological field, can solve the problems of poor specificity, lack, and sensitivity to be improved, and achieve strong immunogenicity and immune protection, improved specificity and sensitivity, and high specificity. Effect
CN102183649AInactive Publication Date: 2011-09-14CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2011-09-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a bovine tuberculosis antigen specific gamma-interferon enzyme-linked immuno sorbent assay (ELISA) kit. The kit comprises a bovine gamma-interferon monoclonal antibody coated ELISA plate, enzyme-labeled bovine gamma-interferon monoclonal antibodies, and protein solution containing mycobacterium bovis MPB83 and MPB70 and mycobacterium tuberculosis ESAT6 and CFP10. The antibodies are captured and detected for different antigen surfaces of gamma-interferon respectively. A bovine tuberculosis antigen specific gamma-interferon ELISA detection method established by using the mycobacterium bovis recombinant proteins MPB83 and MPB70 and mycobacterium tuberculosis recombinant proteins ESAT6 and CFP10 is relatively stable; the specificity of the method is 96 percent, and the sensitivity of the method is 88.6 percent; and the specificity and the sensitivity of the gamma-interferon ELISA detection method for the bovine tuberculosis are greatly improved.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, in particular, it relates to the cloning, expression and purification of four recombinant proteins of Mycobacterium bovis MPB83, MPB70, Mycobacterium tuberculosis ESAT6 and CFP10, and the determination of the optimal concentration of the four proteins as multiple combinations of diagnostic antigens, Expression of bovine IFN-γ recombinant protein, preparation and application of its monoclonal antibody. Background technique

[0002] Tuberculin skin test (TST) has been used to detect bovine tuberculosis, but its sensitivity and specificity are low, and it is difficult to detect early bovine tuberculosis infection. IFN-γ, as the main indicator of Th1-type cellular immune effect, is significantly increased in the early stage of Mycobacterium bovis infection, which points out the direction for the development of effective detection reagents for early bovine tuberculosis.

[0003] There are domestic resear...

Claims

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