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Method for extracting biotin from Antarctic krill and high-efficiency thin layer scanning analysis method

An Antarctic krill and analysis method technology, which is applied in analysis materials, material separation, preparation of test samples, etc., can solve the problems of troublesome operation, high cost, and large amount of solvent of microbial methods, and achieves increased detection sensitivity and better separation. And the effect of simple detection and detection method

Inactive Publication Date: 2012-10-17
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbiological method is cumbersome and time-consuming (it takes 2-3 days to detect a sample), while the HPLC method requires a large investment, a large amount of solvent, and a high cost

Method used

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  • Method for extracting biotin from Antarctic krill and high-efficiency thin layer scanning analysis method
  • Method for extracting biotin from Antarctic krill and high-efficiency thin layer scanning analysis method
  • Method for extracting biotin from Antarctic krill and high-efficiency thin layer scanning analysis method

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Dry the frozen Antarctic krill at 40°C, then stir and extract at room temperature for 30 minutes according to the ratio of shrimp powder (W): chloroform (V) = 1:3 (g:ml), and then to filter. Weigh 1 g of the processed Antarctic krill powder, add 25 ml of dimethylformamide, and carry out stirring extraction in a water bath at a temperature of 75° C. for 1 hour. The extract was separated in a high-speed centrifuge (10,000 rpm) for 10 minutes, the supernatant was taken out, added an equal amount of ethanol, and concentrated by rotary evaporation at 50°C. Spot 4 μl of the concentrated extract solution (i.e. sample solution) on the GF coated with silica gel 254 On a high-efficiency thin-layer plate (10cmX10cm), and use the external standard one-point method to point the biotin standard to 1μI (0.19, 0.3, 0.66, 1.28, 3.31mg / ml). The developing agent is dichloromethane (v): isopropanol (v): methanol (v): glacial acetic acid (v) = 1:1:2:0.01, unfolded in a 10cmX12cmX15cm expa...

Embodiment 2

[0033] The frozen Antarctic krill was dried at 50°C, and then stirred and extracted twice at room temperature according to the ratio of shrimp powder (W): chloroform (V) = 1:7, each time was 40 minutes, and then filtered. Weigh 1 g of the processed Antarctic krill powder, add 35 ml of dimethylformamide, and carry out stirring and extraction in a water bath at a temperature of 85° C. for 1.5 hours. The extract was separated in a high-speed centrifuge (10,000 rpm) for 10 minutes, the supernatant was taken out, added an equal amount of ethanol, and concentrated by rotary evaporation at 55°C. Spot 4 μl of the concentrated extract solution (i.e. sample solution) on the GF coated with silica gel 254 On a high-efficiency thin-layer plate (10cmX10cm), and use the external standard one-point method to point the biotin standard to 1μI (0.19, 0.3, 0.66, 1.28, 3.31mg / ml). The developing agent is dichloromethane (v): isopropanol (v): methanol (v): glacial acetic acid (v) = 2:2:2.5:0.1, un...

Embodiment 3

[0035]The frozen Antarctic krill was dried at 60°C, and then according to the ratio of shrimp powder (W): chloroform (V) = 1:9, stirred and extracted at room temperature for 3 times, each time was 60 minutes, and then filtered. Weigh 1 g of the processed Antarctic krill powder, add 40 ml of dimethylformamide, and carry out stirring extraction in a water bath at a temperature of 95° C. for 2 hours. The extract was separated in a high-speed centrifuge (10,000 rpm) for 10 minutes, the supernatant was taken out, added an equal amount of ethanol, and concentrated by rotary evaporation at 65°C. Spot 4 μl of the concentrated extract solution (i.e. sample solution) on the GF coated with silica gel 254 On a high-efficiency thin-layer plate (10cmX10cm), and use the external standard one-point method to point the biotin standard to 1I (0.19, 0.3, 0.66, 1.28, 3.31mg / ml). The developing agent is dichloromethane (v): isopropanol (v): methanol (v): glacial acetic acid (v) = 3:3:3:0.15, unfo...

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Abstract

The invention discloses a method for extracting biotin from Antarctic krill and a high-efficiency thin layer scanning analysis method. The method comprises the steps of: extracting biotin from Antarctic krill by using dimethyl formamide; centrifuging, removing impurities and concentrating to prepare a sample solution; spreading the biotin standard sample solution on a high-efficiency thin-layer plate coated with silica gel GF254 by adopting an external standard one point method, wherein a spreading agent is a mixed solution prepared from methylene dichloride, isopropanol, methanol and glacial acetic acid in proportion; spraying for dying the spread spradig plate by adopting a dying solution; putting the plate into a fume cupboard, and after five minutes, spraying a 10% of paraffin-chloroform solution; and carrying out thin-layer scanning with an absorption wavelength of 530nm, and calculating the content of the biotin in the Antarctic krill. The method has the advantages of simplicity, accuracy, rapidness, reliability and stability.

Description

technical field [0001] The invention relates to a method for extracting biotin from Antarctic krill and a high-efficiency thin-layer scanning analysis method. Background technique [0002] Antarctic krill (scientific name Euphausia superba), also known as large krill or Antarctic krill, is a kind of krill that lives in the waters of Antarctica. Antarctic krill are shrimp-like invertebrates that live in colonies, sometimes at densities of 10,000-30,000 individuals per cubic meter. They feed on tiny phytoplankton, grow to about 6 cm long, weigh 2 grams, and have a lifespan of 6 years. They are the key species of the Antarctic ecosystem, and in terms of biomass, they may be the most successful animal species on the planet (approximately 500-5000 million tons in total, with large differences due to different estimation methods). Because of the huge reserves of Antarctic krill and the fact that the population is easy to catch, many countries are attracted to develop it. Curren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N30/90
Inventor 张北山刘代成
Owner SHANDONG NORMAL UNIV
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