Method for extracting and detecting biotin of bird nest

A detection method, biotin technology, applied in the direction of measurement device, test sample preparation, color/spectral characteristic measurement, etc., can solve the problems of large investment in high-efficiency liquid phase methods, troublesome operation of microbial methods, and large amount of solvents, etc., to achieve Overcoming cumbersome detection steps, simple detection methods, and the effect of overcoming cumbersome steps

Inactive Publication Date: 2012-07-04
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbiological method is cumbersome and time-consuming (it takes 2 to 3 days to detect a sample), while the HPLC method requires a large investment, a large amount of solvent, and a high cost

Method used

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  • Method for extracting and detecting biotin of bird nest

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Experimental program
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Effect test

Embodiment 1

[0030] Weigh 1 gram of bird's nest powder that has been removed from impurities, add 40ml of dimethylformamide, Stir and extract in a water bath for 1 h. The extract was centrifuged with a high-speed centrifuge (10,000rpm) for 10min. Remove the supernatant and add an equal amount of ethanol, at 65 Concentrate by rotary evaporation. Spot 15 μl of the concentrated extract (i.e. sample solution) on a silica gel 60 F254 high-efficiency thin-layer plate (10cm×10cm), and use the external standard one-point method to spot the biotin standard at 1 l (1.48mg / ml). The developer is chloroform (v): acetone (v): methanol (v): glacial acetic acid (v) = 2.5: 1.5: 2: 0.01, unfolded in a 10cm × 12cm × 15cm expansion cylinder, the expansion distance is 6cm, and the development time is 20min. After unfolding, the plate was dried first, and then stained with 0.05% potassium permanganate staining solution. Put the dyed board into 100 After drying in an oven for 12 minutes, the Rf value ...

Embodiment 2

[0032] Weigh 1 gram of bird's nest powder that has been removed from impurities, add 25ml of dimethylformamide, The water bath was stirred and extracted for 2h. The extract was centrifuged with a high-speed centrifuge (10,000rpm) for 10min. Remove the supernatant and add an equal amount of ethanol at 50 Concentrate by rotary evaporation. Spot 15 μl of the concentrated extract (i.e. sample solution) on a silica gel 60 F254 high-efficiency thin-layer plate (10cm×10cm), and use the external standard one-point method to spot the biotin standard at 1 I (1.48 mg / ml). The developing agent is chloroform (v): acetone (v): methanol (v): glacial acetic acid (v) = 1.5: 2.5: 1: 0.1, unfolded in a 10cm×12cm×15cm developing cylinder, the spreading distance is 6cm, and the developing time is 20min. After unfolding, the plate was dried first, and then stained with 0.05% potassium permanganate staining solution. Put the dyed plate into 120 Dry in an oven for 8 minutes, and the Rf val...

Embodiment 3

[0034] Weigh 1 gram of bird's nest powder that has been removed from impurities, add 30ml of dimethylformamide, Stir and extract in a water bath for 1.5 hours. The extract was centrifuged with a high-speed centrifuge (10,000rpm) for 10min. Remove the supernatant and add an equal amount of ethanol, at 60 Concentrate by rotary evaporation. Spot 15 μl of the concentrated extract (i.e. sample solution) on a silica gel 60 F254 high-efficiency thin-layer plate (10cm×10cm), and use the external standard one-point method to spot the biotin standard at 1 l (1.48mg / ml). The developer is chloroform (v): acetone (v): methanol (v): glacial acetic acid (v) = 2.0: 2.0: 1.5: 0.05, and it is developed in a 10cm × 12cm × 15cm expansion cylinder, the expansion distance is 6cm, and the development time for 20min. After unfolding, the plate was dried first, and then stained with 0.05% potassium permanganate staining solution. Put the dyed plate into 110 After drying in an oven for 10 min...

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Abstract

The invention provides a method for extracting and detecting a biotin of a bird nest. The method comprises the following steps of: extracting the biotin from the bird nest by using dimethylformamide; performing centrifugal purification and concentration to prepare a sample solution; by using a one-point external standard method, developing the sample solution and a biotin standard solution on a high-performance thin-layer plate coated with silica gel 60F254 by using a developer, wherein the developer is a mixture of chloroform, acetone, methanol and glacial acetic acid; spraying 0.05 percent of potassium permanganate solution to a developed plate for coloring; and drying the developed plate, and then performing thin layer scanning with absorption wavelength of 400 nm to obtain the contentof the biotin in the bird nest. The method has the advantages of simplicity, accuracy, quickness, stability and reliability, and the defects of complicated detection steps and high time consumption of the conventional microbiological method, and high investment cost, large solvent dosage and high cost of a high performance liquid chromatography are overcome.

Description

technical field [0001] The invention relates to a method for extracting and detecting bird's nest biotin, in particular to a method for rapid extraction and high-efficiency thin-layer detection and analysis of bird's nest biotin. Background technique [0002] Bird's nest is mainly produced in Malaysia and Indonesia. It is an extremely valuable Chinese medicinal material and health care product raw material. The sialic acid in bird's nest is also a natural product that is effective in preventing influenza virus, thereby strengthening the function of the immune system. The main components of bird's nest are protein (62-63%), carbohydrates (25.62-27.26%), ash (2.1%) and oil (0.14-1.28%). The main amino acids in bird's nest are aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, tyrosine, phenylalanine, Lysine, histidine, arginine, and cysteine, etc. Due to the high nutritional value of bird's nest and the low yield, con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/94G01N21/33G01N1/40G01N1/44
Inventor 刘代成张北山
Owner SHANDONG NORMAL UNIV
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