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Method for detecting total ginkgolic acid in ginkgo leaf extract

A technology of total ginkgolic acid and ginkgo biloba, applied in the field of chemical analysis, can solve the problem that the total ginkgolic acid cannot be accurately determined in samples, etc.

Inactive Publication Date: 2017-11-03
HEILONGJIANG ZBD PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing detection method of total ginkgolic acid cannot accurately determine the content in the sample. Therefore, it is extremely important to establish a more accurate and convenient analysis method for ginkgolic acid.

Method used

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  • Method for detecting total ginkgolic acid in ginkgo leaf extract
  • Method for detecting total ginkgolic acid in ginkgo leaf extract
  • Method for detecting total ginkgolic acid in ginkgo leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] The batch number B20160301 produced by Heilongjiang Zhenbaodao Pharmaceutical Co., Ltd. Jixi Branch is taken as the ginkgo leaf extract, and the detection method for detecting total ginkgolic acid in the ginkgo leaf extract comprises the following steps:

[0108] 1) Preparation of reference substance solution: Accurately weigh an appropriate amount of ginkgoic acid reference substance, and add it into 80% by volume methanol solution, wherein the addition ratio is: add 1 μg of ginkgoctate reference substance per 1 mL of 80% methanol solution.

[0109] In addition, prepare the reference substance solution for positioning again: accurately weigh an appropriate amount of total ginkgolic acid reference substance, and add it into a methanol solution with a volume percentage of 80%. Control solution for positioning.

[0110] 2) Preparation of the test solution: the sample ginkgo leaf extract was made into solid powder by spray drying method, and 0.5 g of the sample powder was ...

Embodiment 2

[0119] The methanol solution with a percentage of 70%, the methanol solution with a percentage of 80%, the methanol solution with a percentage of 90% and pure methanol were respectively selected as solvents, and the experimental comparison of the solvents was carried out;

[0120] The same experimental method as in Example 1 was used to prepare the ginkgocin reference solution and the test solution, only the percentage of the methanol solution was different, and they were detected by high performance liquid chromatography. Investigate the above-mentioned methanol solvents of different percentages by measuring the response factor of the reference substance solution and the symmetry of the chromatographic peak, as shown in Table 1:

[0121] Table 1 Comparison of different concentrations of methanol

[0122]

[0123]

[0124] As can be seen from the above-mentioned numerical values, the peak shapes of the need testing solution of 70% methanol solution, 80% methanol solution...

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Abstract

The invention relates to a method for detecting total ginkgolic acid in ginkgo leaf extract. The method comprises the following steps: preparing a test solution; preparing a reference solution; separately detecting the test solution and the reference solution by using high performance liquid chromatography so as to obtain chromatograms respectively; and calculating the content of total ginkgolic acid in the to-be-detected ginkgo leaf extract by using an external standard method according to the area of total ginkgolic acid in the reference chromatogram and the area of total ginkgolic acid in the test chromatogram. According to the invention, a method for preparing a sample solution is simple and convenient to operate; and the detection method is stable, uniform, controllable and convenient, and can quickly and accurately reflect the real situation of the to-be-detected ginkgo leaf extract.

Description

technical field [0001] The invention relates to the field of chemical analysis, in particular to a method for detecting total ginkgolic acid in ginkgo leaf extract. Background technique [0002] Ginkgo biloba is known as "the living fossil of the plant kingdom". It is native to China, and the possession of ginkgo resources in China accounts for 70% of the world's total. Ginkgo biloba is the dry leaf of Ginkgo biloba (Ginkgo biloba L.), sweet, bitter, astringent, flat, homecoming, lung meridian, has the effects of astringing the lungs, relieving asthma, promoting blood circulation, removing blood stasis, and relieving pain; it is often used to treat coronary heart disease , angina pectoris and hyperlipidemia. Ginkgo biloba extract, which is processed from ginkgo leaves, is the best-selling medicine and health care product at home and abroad; as a natural medicine, it has very significant effects in the treatment of cardiovascular and cerebrovascular diseases and Alzheimer's ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8634
Inventor 方同华代喜国席桂才常英杰
Owner HEILONGJIANG ZBD PHARMA
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