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Traditional Chinese medicine composition of bone healing medicine, preparing method thereof and detecting method thereof

A detection method and composition technology, applied in the field of traditional Chinese medicine, can solve problems such as low accuracy and sensitivity of thin-layer chromatography, affecting application, unstable types and contents of active ingredients, etc.

Active Publication Date: 2012-07-18
陕西天地人和药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the accuracy and sensitivity of thin-layer chromatography detection are not high, and only detecting the content of one active ingredient can not accurately and comprehensively reflect the active ingredient content and drug efficacy of the medicine, which leads to the prepared Guyuling Chinese medicine composition The type and content of active ingredients are unstable, which affects clinical application

Method used

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  • Traditional Chinese medicine composition of bone healing medicine, preparing method thereof and detecting method thereof
  • Traditional Chinese medicine composition of bone healing medicine, preparing method thereof and detecting method thereof
  • Traditional Chinese medicine composition of bone healing medicine, preparing method thereof and detecting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The preparation of embodiment 1 Guyuling capsule

[0054] The prescription and preparation method are as follows:

[0055] Prescription (1000 capsules): 60g Panax notoginseng; 60g dried blood; 30g safflower; 20g frankincense (made); 20g rhubarb; 20g angelica; 20g Chuanxiong; 20g myrrh (made); 20g; Rhizoma Drynaria 20g; Dipsacus 20g; Natural Copper (calcined) 20g; Wujiapi 20g and borax 20g.

[0056] The above sixteen flavors are mixed and pulverized into fine powder, dried, mixed evenly, packed into capsules, and obtained.

Embodiment 2

[0057] Notoginsenoside R in Example 2 Guyuling Capsules 1 , Ginsenoside Rg 1 and ginsenoside Rb1 content detection

[0058] In this example, the notoginsenoside R in the Guyuling capsules prepared in Example 1 was detected by high performance liquid chromatography. 1 , Ginsenoside Rg 1 and ginsenoside Rb 1 content.

[0059] Instrument: high performance liquid chromatography, Alltech, USA, model UVIS-201, UV detector.

[0060] Reagents: Acetonitrile is chromatographically pure reagent; water is ultrapure water; other reagents are analytically pure.

[0061] Reference substance: Ginsenoside Rb 1 (110704-200318), Ginsenoside Rg 1 (110703-200424) and notoginsenoside R 1 (110745-200414) were purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0062] The corresponding instruments, reagents and reference substances involved in each of the following examples are the same.

[0063] 1. Chromatographic conditions

[0064] Chromatographic...

Embodiment 3

[0097] The detection of the content of hydroxysafflor yellow A in embodiment 3 Guyuling capsules

[0098] In this example, the content of hydroxysafflower yellow A in the Guyuling capsules prepared in Example 1 was detected by high performance liquid chromatography.

[0099] 1. Chromatographic conditions:

[0100] Chromatographic column: C18 chromatographic analysis column (4.6mm×250mm, 5μm), guard column (ODS, 4.0mm×3.0mm); mobile phase: methanol-acetonitrile-0.7% (volume ratio) phosphoric acid aqueous solution (volume ratio=62:2 : 72); UV detection wavelength: 403nm; flow rate: 1.0ml / min; column temperature: 30C.

[0101] Under the above chromatographic conditions, the separation between the reference product hydroxysafflor yellow A and the adjacent chromatographic peak is greater than 1.5, and the number of theoretical plates is not less than 1500 according to the peak of hydroxy safflower yellow A.

[0102] 2. Preparation of reference solution

[0103] Accurately weigh ...

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Abstract

The invention provides a traditional Chinese medicine composition of a bone healing medicine, a preparing method thereof and a detecting method thereof. Each preparation unit of the traditional Chinese medicine composition contains pseudo-ginseng which is no less than 2.2mg by total content of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 and / or Carthamus tinctorius which is no less than 0.03mg by content of hydroxysafflor yellow A, quality is more stable, and curative effect is more exact. According to the detecting method, the content of the notoginsenoside R1, the ginsenoside Rg1, the ginsenoside Rb1 and / or the content of the hydroxysafflor yellow A are detected through detecting methods including a high-performance liquid chromatography method. The method is simple and stable, high in accuracy and good in reproducibility, and capable of representing effective ingredient content of the traditional Chinese medicine composition of the bone healing medicine completely and effectively and benefiting monitoring product quality.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a traditional Chinese medicine composition for treating fractures and osteoporosis, a preparation method and a detection method thereof, in particular to a Guyuling traditional Chinese medicine composition, a preparation method and a detection method thereof. Background technique [0002] The prescription of Guyuling traditional Chinese medicine composition comes from the secret prescription of bone diseases and fractures handed down by Li Diansheng, a famous orthopedic doctor in Weibei, Shaanxi. The drug is successfully developed after several years of hard research and clinical trials based on modern pharmacological theory. good medicine. [0003] The standard source of Guyuling traditional Chinese medicine composition is WS-10015(ZD-0015)-2002. It stipulates that the contents of the capsules are light brown to brown-red powder with slightly fragrant gas and slightly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/889G01N30/02G01N30/90A61P19/08A61K33/22A61K33/26
Inventor 朱加武胡迎花苏敏刘素华温锦荣张敏
Owner 陕西天地人和药业有限公司
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