36results about How to "Rapid separation and enrichment" patented technology

The invention discloses a magnetic carbon nanotube composite material and a preparation method and an application thereof. The preparation method comprises the following steps: adding FeCl3.6H2O to an ethylene glycol solution; then adding anhydrous sodium acetate and polyethylene glycol; adding a carboxylated multiwalled carbon nanotube; performing ultrasonic dispersion; adding hexamethylenediamine or ethanediamine; heating to 200-300 DEG C for reaction for 8-24h; and washing and performing vacuum drying to prepare the amino-modified magnetic Fe3O4-carbon nanotube composite material. The amino-modified magnetic Fe3O4-carbon nanotube composite material prepared by the invention is of nanoscale and superparamagnetism, can be stably dispersed in solutions, and can be quickly separated and enriched through a simple action of a magnetic field. As an adsorbent, the material is large in superficial area and has various active groups on the surface. The material can adsorb pigments from complex substrates through pi-pi electron interaction and hydrophobic effect of the carbon nanotube and can adsorb compounds such as organic acids and phenols through weak anion exchange effect of amino groups on the surfaces of magnetic nanoparticles.

The invention provides a preparation method for a molecular imprinting material and the molecular imprinting material prepared through the preparation method. The preparation method comprises the steps that silicon oxide is coated on the surfaces of magnetic ferroferric oxide nanometer particles, the magnetic ferroferric oxide nanometer particles are modified with gamma-(methacryloyl chloride) amino propyl trimethoxy silane to obtain magnetic ferroferric oxide nanometer particles with propenyl on the surfaces, the magnetic ferroferric oxide nanometer particles with the propenyl on the surfaces serve as carriers, estrogen receptors are simulated, functional monomers are optimized, a surface imprinting technology is adopted, and then the molecular imprinting material which can simultaneously identify seven kinds of environmental endocrine disrupting chemicals is prepared. According to the preparation method, the easy separation of a magnetic nanometer material, the good water solubility of a silicon oxide nanometer material, the specific recognition ability of molecular imprinting polymers and the surface imprinting technology are mutually combined, the preparation technology is simple, the conditions are mild, the prepared molecular imprinting material is large in adsorption capacity, fast to respond, high in magnetism, good in chemical stability and high in repeating utilization rate, and the problems that at present, multiple trace, steroid and phenol environmental endocrine disrupting chemicals are difficult to simultaneously identify, separate and enrich are solved.

The invention discloses a method for preparing a magnetic molecularly imprinted polymer for separating atrazine. The method comprises the following steps of: 1) activating Fe3O4atSiO2 particles, and performing surface modification by using a silane coupling agent to obtain composite magnetic nano-particles; and 2) adding the atrazine and methacrylic acid into an organic solvent and preassembling,adding the composite magnetic nano-particles, a crosslinking agent and an initiator, performing polymerization reaction at the temperature of between 60 and 70 DEG for 20 to 24h in an anaerobic environment, and removing the atrazine to obtain the magnetic molecularly imprinted polymer. The method is easy to operate and is suitable for large-scale production; the time consumption is low; raw materials are cheap and readily available; and membrane materials with micron-scale and nano-scale diameters can be obtained. The invention also provides the magnetic molecularly imprinted polymer preparedby the method. Magnetic molecules are firmly combined, and the magnetic molecularly imprinted polymer is high in stability and has stronger adsorption capacity when separating the atrazine.

The invention relates to preparation of a tetracycline antibiotic (tetracycline, oxytetracycline, aureomycin and doxycycline) multi-template molecularly imprinted magnetic composite material and application of the composite material in removal and detection of tetracycline antibiotics. After the surface of magnetic graphene oxide is coated with mesoporous silica, tetracycline, oxytetracycline, aureomycin and doxycycline are jointly used as template molecules, anilinomethyltriethoxysilane and N-[3-(trimethoxysilyl) propyl] ethylenediamine are jointly used as functional monomers, and a sol-gel method is adopted for surface molecular imprinting. The obtained tetracycline antibiotic multi-template molecularly imprinted magnetic composite material can be used for carrying out high-selectivity adsorption on tetracycline, oxytetracycline, aureomycin and doxycycline in a solution at the same time; the adsorption capacities are 5.9 mg/g, 5.7 mg/g, 19.5 mg/g and 11.9 mg/g respectively, adsorption equilibrium is achieved within 60 min, the adsorbent can be repeatedly used five times or more, and the material can be used for removing and detecting tetracycline antibiotics.

The invention relates to L-glutamic acid terminated polypeptide MIP (molecular imprinting polymer) magnetic microspheres as well as a preparation method thereof. The preparation method comprises stepsas follows: a multifunctional magnetic CuFeMnO4 nano affine material is synthesized with a hydrothermal method, the strong coordination effect of Cu<2+> and Fe<3+> in the affine material with carboxyl and amino in polypeptide is used, the affine material serves as a carrier, alternative template molecules, functional monomers, an RAFT reagent, a crosslinking agent and an initiator are added to awater-phase system, molecular imprinting is performed on the surface of the CuFeMnO4 nano material with a one-step method, and MIPs@CuFeMnO4 microspheres are obtained after an RAFT polymerization reaction; and alternative template molecules embedded in the polymer are removed through elution, and the MIPs@CuFeMnO4 microspheres with MIP rigid identification holes are obtained. The MIPs@CuFeMnO4 magnetic affine nano-microspheres prepared with the method have the advantages of uniform surface coating, narrower imprinting polymer molecular weight distribution, stable extraction performance, high extraction selectivity and the like.

The invention discloses a kit based on immunomagnetic bead separation, and application of the kit. The invention further discloses a method for detecting the concentration of escherichia coli based on a visual BPE-ECL technology. The invention further discloses application of the kit and the detection method in detection of the concentration of escherichia coli in food. According to the established method for detecting the concentration of the escherichia coli through the visual BPE-ECL technology, whether the escherichia coli exists in a sample to be detected or not can be observed through naked eyes, the method has the advantages of being simple in principle, rapid (the experimental period is 45 min), high in specificity and high in detection sensitivity (the detection limit is 101 CFU/mL), the method is easy and convenient to operate, actual liquid samples (milk and tap water) do not need to be preprocssed, and a solid actual sample (minced beef) is simply preprocessed according to the national standard; and meanwhile, biochemical modification does not need to be carried out on the surface of a bipolar electrode, and the electrode can be repeatedly used, so the detection cost can be effectively reduced.

The invention relates to the field of separation of organic pollutants in a water environment, and particularly discloses a method for separating and enriching sulfonamide antibiotics in water. The method comprises the following steps: 1) sealing one end of a hollow fiber membrane, and loading an extraction solvent on the hollow fiber membrane; 2) injecting a receptor solution into the inner cavity of the hollow fiber membrane; 3) immersing the closed end of the hollow fiber membrane treated in the step 2) in a water sample, inserting a working electrode into the water sample, inserting a counter electrode into the receptor solution, applying voltage to the working electrode and the counter electrode to form an electric field, and extracting sulfonamide antibiotics in the water sample under the action of the electric field; and 4) after the extraction is finished, collecting the receptor solution in the hollow fiber membrane to finish the separation and enrichment of the sulfonamide antibiotics in the water sample. The method disclosed by the invention has the advantages of small organic solvent consumption, high mass transfer rate, high sulfonamide antibiotic recovery rate and wide application prospect.