123 results about "Magnetic molecules" patented technology

A biosensor not requiring washing of an unbonded label molecular by analyzing an object to be measured such as an antigen, an antibody, a DNA, and an RNA through magnetic field sensing. The biosensor is small in size, low in price, and high in sensing accuracy. Semiconductor Hall devices are arrayed two-dimensionally on the bottoms of recesses in the surface of a sensor chip to which bonded is a magnetic molecule with which a magnetic particle is labeled so as to sense the magnetic field produced along the sensor surface of the sensor chip. The surface area of each semiconductor Hall device is less than the maximum cross section of the magnetic molecule, and the intervals of the arrayed semiconductor Hall devices are larger than the diameter of the magnetic Hall molecule. Thus, the analysis accuracy is enhanced. An interconnection is used commonly to the semiconductor Hall devices, thereby reducing the size of the sensor.

The present invention specifically adopts a simple method for preparing a molecularly imprinted polymer and directly applies the molecularly imprinted polymer to the extraction separation of trace substance in the complex sample pre-processing. The invention adopts a chemical coprecipitation method for preparing Fe3O4 magnetofluid. Then the surface of Fe3O4 magnetofluid is modified with oleic acid. The template molecules and functional monomers are realized with preassembling and are mixed with the cross linker and modified magnetofluid. Then the mixture is added into the polymer solvent containing dispersing agent. After the reaction is finished, magnetic field separation is executed. The polymer is dried after eluting the formwork molecules. A three-phase extraction system which is established on the base of magnetic molecularly imprinted polymer can be directly applied to the extraction separation of complex samples of food, environment and biological sample. The method of the invention has excellent selectivity and can eliminate the matrix interference in the sample. Furthermore as the polymer has excellent magnetism, the polymer can excellently realize the separation to the sample matrix after the extraction, and the polymer has broad application prospect in the complex sample pre-processing.

The invention discloses a temperature-sensitive magnetic sulfadimidine molecular imprinted adsorbent as well as a preparation method and application thereof, belonging to the technical field of preparation of environment functional materials. A gamma-Fe2O3 / SiO2 magnetic composite material is prepared by using a sol-gel method and is subjected to vinyl modification by using 3-(methylacryloyloxy) trimethoxypropylsilane; with a vinyl-modified magnetic composite material (MPS (Modified Polystyrene)-gamma-Fe2O3 / SiO2) as a substrate material, sulfadimidine as a template molecule, acrylamide as a functional monomer, N-isopropylacrylamide as a temperature-sensitive monomer, ethylene glycol-bis(methylacrylic acid) ester as a cross-linking agent and 2,2'-azobisisobutyronitrile as an initiator, a temperature-sensitive magnetic molecular imprinted polymer is prepared by using a free group polymerization process and is applied to selective recognition and separation of the sulfadimidine in an aqueous solution; and the obtained polymer has the advantages of remarkable heat and magnet stability, sensitive magnet and heat inducting effect, higher adsorption capacity, reversible adsorption / release function along with the temperature and remarkable recognition performance of a sulfadimidine molecule.

The invention relates to a method for preparing magnetic molecularly imprinted polymers (MIPs) through suspension polymerization, belonging to the technical field of preparation of environmental functional materials. In particular, in the method for preparing the magnetic MIPs through suspension polymerization, Fe3O4 magnetic particles are synthesized through a hydrothermal synthesis method, methacrylic acid is taken as a functional monomer, 2,4,6-trichlorophenol (2,4,6-TCP) is taken as template molecules, azodiisobutyronitrile is taken as an initiator, methylbenzene is taken as a pore-foaming agent, ethylene glycol dimethacrylate is taken as a crosslinking agent, and polyvinyl alcohol is taken as a stabilizing agent. The obtained MIPs are regular spheres, holes of specific shapes and sizes left by a plurality of template molecules are formed in the surfaces of the polymers, and specifically-arranged binding sites are arranged in the holes. By adopting the MIPs, highly-selective adsorption of a target pollutant, namely, 2,4,6-trichlorophenol can be realized.

A decontamination system uses magnetic molecules having ferritin cores to selectively remove target contaminant ions from a solution. The magnetic molecules are based upon a ferritin protein structure and have a very small magnetic ferritin core and a selective ion exchange function attached to its surface. Various types of ion exchange functions can be attached to the magnetic molecules, each of which is designed to remove a specific contaminant such as radioactive ions. The ion exchange functions allow the magnetic molecules to selectively absorb the contaminant ions from a solution while being inert to other non-target ions. The magnetic properties of the magnetic molecule allow the magnetic molecules and the absorbed contaminant ions to be removed from solution by magnetic filtration.

The invention discloses a construction method of an impedance type electrochemical sensor based on a molecularly imprinted polymer with a magnetic surface and an application of the sensor in protein detection, and belongs to the technical field of electrochemical sensing. The construction method comprises the following steps: synthesizing the molecularly imprinted polymer on the surface of a magnetic nano particle by taking hemoglobin as a template molecule and dopamine as a monomer and by using the self polymerization performance and the good adhesive capacity of the dopamine; eluting the hemoglobin, so as to prepare the molecularly imprinted polymer with the magnetic surface, wherein the molecularly imprinted polymer with the magnetic surface has the good identification effect on the hemoglobin; and fixing the polymer on the surface of a magnetic glassy carbon electrode under the action of a magnetic field, so as to construct a hemoglobin sensing interface based on the molecularly imprinted polymer with the magnetic surface, wherein the hemoglobin sensing interface based on the molecularly imprinted polymer with the magnetic surface is used for hemoglobin detection. The constructed electrochemical sensor based on the molecularly imprinted polymer with the magnetic surface is simple in method, high in hemoglobin detection sensitivity and good in selectivity.

The invention relates to an underwater navigating electromagnetism heating cavitation device. It installs a cavitating device formed by magnetic molecule material on the head of the navigating device, and installs a high frequency alternating electromagnetic field generator in navigating device. When the generator works, the magnetic molecule material would generate eddy current to make molecule moving disorderly in high speed. The molecule would collide and fractionize to generate heat, and the temperature would rise rapidly to achieve vaporizing temperature. The bubble would be formed around the navigating device to decrease the surface friction resistance and improve moving speed.

The invention discloses a method for preparing a magnetic molecularly-imprinted adsorbent and belongs to the technical field of preparation of environment-friendly functional materials. The method comprises the following steps of dispersing nanometer chitosan particles serving as stable particles in a solution as a water phase; mixing toluene serving as an oil-phase solvent and a pore-forming agent, methyl methacrylate serving as a functional monomer, ethylene glycol di(methacrylate) serving as a crosslinking agent, 2,2'-azobisisobutyronitrile serving as an initiator and magnetic carrier oil acid-modified nanometer ferriferrous oxide and molecularly-imprinted erythromycin to obtain a mixture serving as an oil phase; mixing the oil phase and the water phase to form a stable Pickering emulsion, preparing magnetically imprinted polymer microspheres by virtue of heat-induced reaction and applying the magnetically imprinted polymer microspheres in the adsorption and separation of erythromycin in an aqueous solution. The product has the imprinted hole and magnetic characteristics and is conducive to specific adsorption of target molecules, the magnetic carrier is conducive to the separation of the adsorbent and internal imprints can prevent the automatic falling and magnetic flux leakage of the target molecules after the target molecules are adsorbed and a better absorption effect is achieved.

The invention relates to a preparation method and an application for magnetic molecular imprinting polymers for selectively separating carbamazepine and belongs to the technical fields of environmental material preparation and pollution regulation. Nanometer ferroferric oxide modified on a surface of chitosan serves as a magnetic medium, molecular imprinting material polymerization is carried out on the surface of the nanometer ferroferric oxide by using a sediment polymerization method, and a magnetic molecular imprinting adsorption agent is obtained after template molecular carbamazepine is eluted. Obtained molecular imprinting composite materials are stable in physicochemical property, have high adsorption capacity and specific identification characteristics on the carbamazepine, have superparamagnetism, can be rapidly separated under an outside magnetic field effect, therefore, interference of a complex substrate in an actual sample is avoided, and sample treatment processes are greatly simplified. The preparation method is simple, reliable and low in cost and has a wide application prospect in analysis, detection and pollution treatment of a complex environment sample.

The invention relates to a novel magnetic molecular targeted ultrasound contrast agent, in particular to a gas-wrapped magnetic liposome microsphere suspension with the mean diameter of 1-4 micrometers and a preparation method thereof. The preparation method of the magnetic molecular targeted ultrasound contrast agent microsphere comprises a condition that a lipid layer of the microsphere and / or the surface of the lipid layer contains (or connects) magnetic response materials. A contrast agent microsphere wall material contains phospholipids, polyethylene glycol (PEG), PEG phospholipid polymers, biotinylated and / or polypeptide modificatory PEG phospholipid polymer, poloxamer, ligands (monoclonal antibodies or polypeptide, and the like) and the magnetic response materials and / or avidin bridging materials, wherein the ligand (monoclonal antibodies or polypeptide, and the like) has specific affinity to target molecules, the wrapped gas is a fluorine carbon gas, and a solvent is an aqueous medium (distilled water). The invention also provides the preparation method of the magnetic molecular targeted ultrasound contrast agent microsphere, which comprises a condition that the specific ligand is connected with the microsphere in a covalence and avidin bridging way. The novel magnetic molecular targeted ultrasound contrast agent has good targeted developing effect, can be used for evaluating the change of vessel endothelial molecules of an arterious and venous system of an organic tissue and has good application prospect in treatment.

The invention relates to a preparation method for a novel magnetic molecular sieve adsorbent material which is used for removing antibiotic pollutants in water treatment, has good adsorption performance and can be easily separated by using a magnetic separation method. The preparation method comprises the following steps: mixing soluble manganese salt and ferric salt according to a mol ratio of 1: 2, adding alkali to neutralize an obtained mixture and then carrying out microwave-assisted hydro-thermal synthesis, crystallization, magnetic field separating, rinsing and drying so as to obtain a ferromanganese magnetic spinel material; adding prepared magnetic spinel into a mixed solution consisting of cetyltrimethylammonium bromide, ethanol, ammonia water and deionized water, carrying out ultrasonic and vigorous stirring, adding ethyl orthosilicate, then continuing stirring for a reaction and then successively carrying out pumping filtration, rinsing with pure water, drying under vacuum, grinding and calcining under the protection of nitrogen so as to prepare the novel magnetic molecular sieve adsorbent material. The magnetic molecular sieve adsorbent material has the characteristics of rapid adsorption, high efficiency magnetic separation and a high adsorption removal rate when used as an adsorbent for removal of tetracycline antibiotic pollutants like terramycin and aureomycin in waste water and is suitable for popularization and application.

The invention discloses a preparation method and application of hydrophilic magnetic molecule imprinted material for sulfonamides veterinary drugs, belongs to the field of organic matter inspection, and particularly relates to a preparation method and application of the sulfonamides drug molecule imprinted material. The method includes the steps of 1), preparing Fe3O4 particles with a coprecipitation method; 2), preparing Fe3O4 for silylation by adopting a stober method to obtain SiO2 coated magnetic microspheres; 3), synthesizing sulfonamides molecule printed polymer particles. The magnetic molecule imprinted materials is fast in adsorption capacity in water phase, high in adsorption function, strong in specificity, high in recovery rate and low in preparation cost; meanwhile, the shortcoming that large amount of solvent required in pretreatment technology of traditional samples is overcome.

The invention discloses a method for preparing a magnetic molecularly imprinted polymer for separating atrazine. The method comprises the following steps of: 1) activating Fe3O4atSiO2 particles, and performing surface modification by using a silane coupling agent to obtain composite magnetic nano-particles; and 2) adding the atrazine and methacrylic acid into an organic solvent and preassembling,adding the composite magnetic nano-particles, a crosslinking agent and an initiator, performing polymerization reaction at the temperature of between 60 and 70 DEG for 20 to 24h in an anaerobic environment, and removing the atrazine to obtain the magnetic molecularly imprinted polymer. The method is easy to operate and is suitable for large-scale production; the time consumption is low; raw materials are cheap and readily available; and membrane materials with micron-scale and nano-scale diameters can be obtained. The invention also provides the magnetic molecularly imprinted polymer preparedby the method. Magnetic molecules are firmly combined, and the magnetic molecularly imprinted polymer is high in stability and has stronger adsorption capacity when separating the atrazine.

A biosensor not requiring washing of an unbonded label molecular by analyzing an object to be measured such as an antigen, an antibody, a DNA, and an RNA through magnetic field sensing. The biosensor is small in size, low in price, and high in sensing accuracy. Semiconductor Hall devices are arrayed two-dimensionally on the bottoms of recesses in the surface of a sensor chip to which bonded is a magnetic molecule with which a magnetic particle is labeled so as to sense the magnetic field produced along the sensor surface of the sensor chip. The surface area of each semiconductor Hall device is less than the maximum cross section of the magnetic molecule, and the intervals of the arrayed semiconductor Hall devices are larger than the diameter of the magnetic Hall molecule. Thus, the analysis accuracy is enhanced. An interconnection is used commonly to the semiconductor Hall devices, thereby reducing the size of the sensor.

The invention provides a pseudo template magnetic molecule imprinted polymer for pesticide residue detection of a waterborne sample. The polymer Fe3O4@MIPs is prepared by comprising the steps that oleic acid-coated Fe3O4 nanoparticles, pseudo template molecules and functional monomers MAA are added into pore-forming agents, and after self-assembly is performed, cross-linking agents EGDMA and initiating agents AIBN are added in the mixture; after the cross-linking agents EGDMA and the initiating agents AIBN are completely dissolved, the mixture is dropwise added into polyvinyl pyrrolidone water solutions to complete a polymerization reaction; after Fe3O4@MIPs containing the pseudo template molecules is separated through an external magnetic field, the pseudo template molecules are removed, vacuum drying is performed, and the polymer is prepared. According to the polymer, the Fe3O4 nanoparticles are coated with oleic acid, and therefore the agglomeration phenomenon in the Fe3O4 preparation process is effectively avoided; a molecular imprinting polymerization reaction is performed by taking a compound of which the structure is similar to that of target pesticide as the pseudo template molecules, and therefore the phenomenon that a high result or a false positive result caused by the template leakage problem is effectively avoided; the target pesticide is easily and quickly separated from and brought away from the liquid sample through the magnetism of the polymer; the polymer is high in selectivity, little in interference, easy to operate, capable of being reused after being eluted, suitable for being used in the waterborne sample and good in effect.

The invention discloses a magnetic molecularly-imprinted polymer for identifying and enriching varieties of trace antibiotics and a preparation method and application thereof. The method comprises the following steps of mixing multiple types of template molecules and functional monomers, then, carrying out prepolymerization, then, adding surface modified Fe3O4 magnetic fluid, a cross-linking agent and an initiating agent so as to carry out polymerization reaction, collecting a polymer, which is obtained after the polymerization reaction is ended, with a magnetic substance, eluting the template molecules, then, washing with deionized water, and then, drying, thus obtaining the magnetic molecularly-imprinted polymer for identifying and enriching various of trace antibiotics. According to the magnetic molecularly-imprinted polymer for identifying and enriching varieties of trace antibiotics and the preparation method and application thereof, the varieties of trace antibiotics can be selectively identified and enriched and quickly, simply and conveniently separated at one time, so that the cost and time are greatly reduced, the efficiency is effectively increased, and the magnetic molecularly-imprinted polymer has a broad application prospect in the pretreatment of complicated samples in the fields of food, environment and the like.

The invention discloses an application of a magnetic molecular imprinting technique in a chiral recognition of a microfluidic system, provides a new method of constructing a polydimethylsiloxane (PDMS) microfluidic chip system with selective recognition reaction by using the magnetic molecular imprinting technique to a chiral material, and belongs to the field of microfluidic chip technique. The new method of constructing the chiral selective PDMS microfluidic system based on the magnetic molecular imprinting technique has a great number of imprinting sites specifically recognizing D / L-tryptophan and can realize selective, quick and efficient separation of the chiral tryptophan. The technique can be applied to the fields such as imprinting, separation and detection of biomacromolecules (protein, polypeptide and nucleotide).

The invention relates to a magnetic molecular imprinting electrochemical sensor for detecting trace sulfadimidine. The magnetic molecular imprinting electrochemical sensor of sulfadimidine is prepared by adopting a magnetic reduced graphene modified magnetic electrode and applying dopamine to carry out self-polymerization. When the concentration of olaquindox is in a range of 10.0nM-200.0nM, an electrochemical signal rising degree has a good linear relation with the concentration of sulfadimidine; and the detection limit of a method reaches 0.1nM. When the sensor is used for detecting an actual sample, the yield reaches 92.6%-101.7%; and with the adoption of the magnetic molecular imprinting electrochemical sensor, the sensitivity and selectivity of sulfadimidine are improved, and the automation of detection of trace sulfadimidine is easy to realize.

The invention provides a magnetic molecularly imprinted material for identifying chlorophyll, a preparation method of the material and an application. The preparation method of the magnetic molecularly imprinted material includes the steps: (1) synthesizing magnetic nano-particles Fe3O4; (2) modifying the surfaces of the magnetic nano-particles Fe3O4 by the aid of MAA (methacrylic acid); (3) synthesizing the magnetic molecularly imprinted material Fe3O4@MIP. The magnetic molecularly imprinted material for identifying the chlorophyll can be used for selectively adsorbing the chlorophyll, is high in adsorption capacity and reusing rate, good in selectivity and stability, high in magnetism, rapid in response and applied to pretreatment of pesticide residue detection samples by serving as an adsorbing agent, common interferents such as the chlorophyll in pesticide residue analysis can be removed, various pesticides in high-pigment agricultural product samples such as Chinese chives, spinach and tea leaves are successfully made, and particularly, pesticide residues of a planar structure are detected.

The invention discloses a preparation method as well as a product and application of a phthalate molecularly imprinted polymer. The preparation method comprises the following steps: step 1: simulating a phthalate molecularly imprinted polymer pre-assembly system through a computer; step 2: preparation of Fe3O4@SiO2 magnetic nanoparticles; step 3: preparation of virtual template phthalate (10-methoxyl-10-oxodecyl); and Step 4: preparation of a magnetic molecularly imprinted polymer. The obtained PAEs magnetic molecularly imprinted polymer is uniform in morphology and can be used to adsorb ten kinds of PAEs at the same time, and the target is separated and enriched in the case of external magnetic field at one time. The printing efficiency and the adsorption performance of MIPs are improved by computer simulation. The rapid separation and multi-residue detection of PAEs in complex samples is achieved, and the PAEs magnetic molecularly imprinted polymer has a good application prospect.

The invention discloses a magnetic field-induced preparation method of a molecularly-imprinted magnetic enrichment material of trace quantity of chlorophenol pollutant molecules in seawater. The preparation method comprises that under magnetic field induction, an epoxy group-functionalized magnetic polymer is prepared by suspension polymerization and in-situ oxidation, a chlorophenol environmental pollutant polyamine template molecule is obtained by an intermolecular hydrogen bonding self-assembling technology, an amino-functionalized magnetic molecularly-imprinted composite material bonding with the template molecule is prepared under magnetic field induction, and in the presence of an acid / alcohol extractant, through pH adjustment, the template molecule is eluted and the chlorophenol molecule-imprinted magnetic composite material is obtained. The preparation method has simple processes and a low cost and realizes uniform particle size distribution and stable properties of the desired product. The molecularly-imprinted magnetic enrichment material can realize high-efficiency and high-selectivity adsorption and removal of trace quantity of chlorophenol pollutants in seawater and can be used for base dispersion-magnetic solid phase extraction detection of trace quantity of chlorophenol pollutants in seawater.

The invention provides a method for preparing a magnetic molecule mark polymer for detecting a PDE-5 inhibitor. The method comprises the flowing steps of: preparing Fe3O4.SiO2 nanoparticles; performing surface grafting on the Fe3O4.SiO2 nanoparticles; performing polymerization reaction; and performing template molecule elution. The magnetic molecule mark polymer prepared by the method has specific recognition ability on template molecules and analog of the template molecules, is easy to recover under an external magnetic field, and can be used for monitoring the PDE-5 inhibitor which is illegally added into functional health-care foods and traditional Chinese treatment medicaments.

The invention designs a method for synthesizing a magnetic resonance imaging contrast agent by applying synthesis of polylactic acid, a CdTe quantum dot and ferroferric oxide. The method comprises the following step: on the basis of selecting ferroferric oxide as a magnetic molecule and a near infrared CdTe quantum dot as an optical imaging molecule, compounding the ferroferric oxide and the near infrared CdTe quantum dot in a polylactic acid microsphere by virtue of a self-assembly mode to prepare the multifunctional magnetic resonance imaging contrast agent with magnetic resonance imaging, fluorescent imaging and biological targeting functions.

The invention discloses an Nd-Fe-B permanent magnet material for a robot. The Nd-Fe-B permanent magnet material comprises the following elements by mass percent: 25-35% of praseodymium neodymium, 0.86-1.12% of boron, 1.6-4.2% of dysprosium, 0.1-0.8% of copper, 1.2-3.6% of cobalt, 0.1-0.6% of aluminum, 0.05-0.35% of zirconium, 0.1-0.9% of niobium, 0.05-0.35% of gallium, 0.2-0.5% of cerium, 0.3-0.6% of gadolinium, 0.2-0.8% of terbium, and 0.1-0.7% of holmium, with the balance being iron. In the Nd-Fe-B permanent magnet material, element matching is optimized, the anisotropic field of a magnetic molecule is effectively improved, the coercivity of the Nd-Fe-B permanent magnet material is improved, the influence on magnetic energy product is reduced, and the performance of the Nd-Fe-B permanent magnet material is greatly improved.

The invention discloses a preparation method and application of a magnetic 5A molecular sieve. The method comprises the steps of adding FeCl2.4H2O and FeCl3.6H2O into water, performing ultrasonic agitation and heating, then adding NH3.H2O to obtain a solid-liquid mixture, then performing magnetic adsorption, washing and drying to obtain magnetic Fe3O4; using sodium aluminate as an aluminum source, sodium silicate asa silicon source, sodium hydroxide as a base source and water as a solvent, adding calcium chloride, citric acid and the magnetic Fe3O4, and preparing the 5A molecular sieve inan ultrasound field. According to the preparation method and application provided by the invention, the magnetic 5A molecular sieve is prepared by usingultrasonic heating and adding an auxiliary agent, the magnetic molecular sieve has the advantages of high specific surface area, strong adsorption property, easinessin separation from the reaction liquid through additionally added magnetic field and the like. The synergistic effect of the ultrasonic wave and the auxiliary agent on the preparation of the magnetic 5A molecular sieve can increase the chemical reaction speed, save energy and protect the environment, and make the prepared magnetic 5A molecular sieve uniform in particle size and regular in morphology.

The invention relates to a method for removing drugs in soil by utilizing magnetism molecular imprinting-electromagnetism grating combination. The drugs are one or more of carbamazepine, clofibric acid or diclofenac. The method comprises the following specific steps of: fully mixing preprocessed soil and a magnetism molecularly imprinted polymer, absorbing the drugs in the soil on the surface of the magnetism molecular imprinting, and separating the water soil and the magnetism molecularly imprinted polymer after reaction through an electromagnetism grid; and absorbing the magnetism molecular imprinting on the grid through a magnetic field generated by the electromagnetism grid after electrification, and effectively separating the magnetism molecular imprinting and the grid. The method provided by the invention has the advantages that the operation is simple, the effect is obvious, the arbamazepine, the clofibric acid and the diclofenac and the like in the soil are removed in one time, the drugs absorbed on the magnetism molecular imprinting are recovered and separated through the electromagnetism grid; a magnetism molecular imprinting technology is utilized to restore the soil polluted by the drugs and has the advantages that the cost is low, the restoring efficiency is high and the like; and an electromagnetism grid device is utilized to recycle the resources, so that secondary pollution is not caused.

The invention relates to a magnetic molecular imprinting electrochemical sensor used for detecting trace sulfadimidine. A magnetic electrode modified by magnetic reduction graphene, dopamine auto-agglutination is adopted, and the sulfadimidine magnetic molecular imprinting electrochemical sensor is prepared. When the concentration of olaquindox is within the range of 10.0 nM-200.0 nM, the rise degree of electrochemical signals has a good linear relation with the concentration of sulfadimidine, and a method detection limit reaches 0.1 nM. The sensor is used for detecting actual samples, and the recovery rate reaches 92.6-101.7%. The sensor improves sensitivity and selectivity of sulfadimidine, and detection of trace sulfadimidine is easy to automate.

The invention discloses a preparation method and application of a targeted enzyme immobilized carrier based on a magnetic molecular imprinting technology. The method comprises the following steps of preparing ferric aminotetroxide; preparing boracic acid modified ferroferric oxide; preparing a molecular imprinting polymer layer by a sol-gel method; removing horseradish peroxidase combined on the molecular imprinting polymer; sealing non-specific adsorption residuals on the surface of the polymers to obtain horseradish peroxidase magnetic molecular imprinting polymers. The method provided by the invention has the advantages that the preparation process is simple; the cost is low; the obtained magnetic molecular imprinting polymers can be used for specifically recognizing the target substance of the horseradish peroxidase, and can also be used for fixing the horseradish peroxidase in complicated samples directly through adsorption; the formed immobilized enzyme can be used for detecting substances such as glucose, sarcosine, trioxypurine and cholesterol capable of being decomposed to produce H2O2.

The invention discloses a sibutramine magnetic molecularly imprinted polymer and a preparation method thereof. The molecularly imprinted polymer is a magnetic molecularly imprinted polymer having a core-shell structure composed of a core made of Fe3O4@SiO2 magnetic nano particles and a shell made of sibutramine molecularly imprinted polymers. The magnetic molecularly imprinted polymer has special absorption on sibutramine, thus interference of other components on sibutramine detection can be reduced; moreover, the magnetic molecularly imprinted polymer can be directionally controlled by an external magnetic field, thus the absorption, washing, desorption can be conveniently completed, the operation is convenient, so the magnetic molecularly imprinted polymer is suitable for detection of sibutramine illegally added in food and active metabolite of sibutramine namely M1 and M2.

The invention discloses a suspension polymerization preparation method of a mixed five-plate magnetic imprinted polymer. The method comprises preparation, modification of magnetic microspheres, preparation of a polyvinyl alcohol dispersant, dissolution, prepolymerization, polymerization, template molecular elution and drying. The preparation method uses five substances as template materials, addsmodified magnetic microspheres in the polymerization process and synthesizes magnetic molecularly imprinted polymer for specifically recognizing a triazole substance, a pyrethroid substance, a triazine substance, a carbamate substance and a phenyl urea substance by a suspension polymerization method. In the method, the preparation conditions are easy to be controlled, and the prepared polymer hasgood stability, high strength, acid and alkali resistance, no need of grinding and column packing, and can adsorb a plurality of substances at the same time, and has high adsorption efficiency. The method is suitable for the preparation of mixed five-template magnetic imprinted polymer microspheres, and the prepared product can be further used for the separation and enrichment of samples during simultaneous analysis of the plurality of pesticide residues in food, feed and other samples.