25 results about "Magnetic molecules" patented technology

The invention designs a preparation method of a magnetic molecular imprinting-aptamer sandwich fluorescence sensor for detecting kanamycin. And the magnetic molecular imprinting composite material Fe3O4 (at) UiO-66 (at) MIP with a specific adsorption target object KANA is prepared. When a detection target object KANA exists, the Fe3O4-coated UiO-66-coated MIP can rapidly and specifically capture the KANA in a short time. Then, a DNA single-stranded aptamer marked with FAM is added, after the aptamer and KANA captured on the surface of the Fe3O4 at-UiO-66 at-MIP are subjected to specific recognition and magnetic separation again, an aptamer probe can enter sediment along with the magnetic composite material Fe3O4 at-UiO-66 at-MIP, the fluorescence intensity of supernate is reduced, and quantitative analysis is conducted according to the fluorescence difference value delta F. When the KANA does not exist, the aptamer cannot enter the precipitate, so that the fluorescence signal intensity is not changed. Double recognition is formed through specific binding of an MIP imprinting cavity and the aptamer, matrix interference is reduced by means of fluorescence characteristics and magnetic separation capacity, the method has the advantages of being high in adsorption capacity, specific in recognition, high in sensitivity, wide in detection range, good in selectivity and the like, and rapid and accurate detection of KANA in a complex sample can be achieved.

This invention discloses a magnetic nanomolecular sieve, its preparation method, and its application in the enrichment of low-abundance proteins. The preparation method of the magnetic nanomolecular sieve proposed in this invention is simple to operate, employing a one-pot method. It simultaneously imparts magnetism while maintaining the surface properties of the sieve, i.e., its protein adsorption capacity, thus preserving its magnetic properties. This provides a simpler, faster, and more efficient technical method for large-scale sample processing. The low-abundance protein enrichment based on magnetic molecular sieves proposed in this invention can be applied to almost all sample types, effectively solving the interference caused by high-abundance proteins on the identification of low-abundance proteins during mass spectrometry detection, and increasing the number of protein identifications by 100%-700%.

The present invention discloses a molecularly imprinted electrochemical sensor and a preparation method and application thereof. A magnetic molecularly imprinted polymer is obtained by ATRP polymerization using a magnetic MOFs material as a carrier; the magnetic molecularly imprinted polymer is modified onto an electrode surface to obtain the molecularly imprinted electrochemical sensor. In the present invention, a silicon dioxide layer is coated on the surface of a magnetic core to effectively prevent the magnetic core from being oxidized or corroded. Hydroxyl groups are introduced by coating polydopamine on the surface of the magnetic core, which facilitates complexation with metal ions during the synthesis of the MOFs material. The introduction of the MOFs material enriches the recognition sites. An initiator for ATRP polymerization is introduced on the surface of magnetic nanoparticles coated with the MOFs material to prepare a molecularly imprinted polymer layer by surface-initiated atom transfer radical polymerization. The sensor has the characteristics of low separation and recovery difficulty, high recognition efficiency and short detection cycle, avoids the risk of template molecule leakage during use, and has high safety and reliability.

The application relates to the technical field of analysis and detection, in particular to a detection method for a strong-toxic new pollutant 6PPD-quinone, which comprises the following steps: adopting a magnetic molecular imprinting polymer to perform specific adsorption on 6PPD-quinone in a water sample to be detected, eluting, and obtaining a sample liquid rich in 6PPD-quinone; and then adopting a single-atom colorimetric detection method to detect the sample liquid. The method realizes the enrichment of the target 6PPD-quinone, simplifies the pretreatment steps, releases the hands of the detection personnel, and realizes the high-sensitivity and high-selectivity detection of the content of 6PPD-quinone by combining the single-atom colorimetric detection method, so that the method can break through the limitation of large instruments and provide a brand-new solution for realizing the on-site, rapid and low-cost monitoring of ultra-trace 6PPD-quinone in the environment.

The application discloses a kind of molecularly imprinted magnetic microspheres for D-dimer high-efficiency detection and a preparation method thereof, and relates to the technical field of molecular imprinting.The method comprises the preparation of pyrazolone functional group modified magnetic nanomicrospheres, dispersing the pyrazolone functional group modified magnetic nanomicrospheres, D-dimer template molecules, functional monomers and crosslinking agent in a phosphate buffer solution, uniformly mixing, and then adding an initiator to carry out a polymerization reaction.After the polymerization reaction is completed, the D-dimer template molecules are eluted and removed, and the molecularly imprinted magnetic microspheres for D-dimer high-efficiency detection are obtained.The application utilizes the synergistic effect of salt bridge interaction and hydrogen bond interaction between arginine residues in D-dimer and pyrazolone functional groups, and successfully prepares magnetic molecularly imprinted microspheres with high recognition specificity for D-dimer.

The invention relates to the technical field of liquid chromatography / mass spectrometry analysis, and discloses a method for improving LC / MS analysis sensitivity, which is suitable for detecting trace lipid metabolites (containing isomeride) of a biological sample. The core technical scheme is as follows: a magnetic molecularly imprinted polymer-solid phase support liquid-liquid extraction secondary purification system is adopted to remove matrix impurities, a gradient mobile phase containing a composite additive is matched to optimize separation, and the detection specificity is improved by an ion mobility spectrometry-segmented selective ion monitoring combined mode; and a stable isotope internal standard is synchronously introduced to correct quantitative deviation. Experiments show that the LOD of the method is as low as 0.12-0.25 ng / mL, the matrix effect is smaller than or equal to 5%, the separation degree and the recovery rate are excellent, low-abundance metabolic marker differences can be accurately captured, and a support is provided for disease diagnosis and metabonomics research.

The invention relates to the technical field of preparation of imprinted polymers, and particularly discloses a magnetic molecularly imprinted polymer for sulfonamides and a preparation method of the magnetic molecularly imprinted polymer for sulfonamides. Tetraethyl orthosilicate is used for silanization of the surface of Fe3O4, then 3-(trimethoxysilyl) propyl methacrylate and silicon hydroxyl of Fe3O4 (at) SiO2 are used for condensation, and the magnetic molecularly imprinted polymer for sulfonamides is obtained. The preparation method comprises the following steps: carrying out vinyl modification on the surface of Fe3O4 (at) SiO2-CH = CH2 particles to obtain Fe3O4 (at) SiO2-CH = CH2 particles, and carrying out copolymerization on the Fe3O4 (at) SiO2-CH = CH2 particles and Fe3O4 (at) SiO2-CH = CH2 by adopting a surface imprinting method and taking sulfadimidine as a template molecule, methyl methacrylate as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent so as to prepare the Fe3O4 (at) SiO2-CH = CH2 molecularly imprinted polymer. The magnetic molecularly imprinted polymer obtained by the invention can quickly identify sulfadimidine under the action of an external magnetic field, is simple to prepare, good in adsorbability, relatively low in cost and convenient to recycle, can be repeatedly utilized, and has a relatively good application prospect.

This invention relates to the field of natural product separation and purification technology, and discloses a magnetic molecularly imprinted material for extracting Ganoderma lucidum polysaccharides and its preparation method. The magnetic molecularly imprinted material includes a magnetic metal-organic framework composite carrier and a molecularly imprinted polymer layer coated on the surface of the composite carrier. The magnetic molecularly imprinted material is prepared by polymerizing raw materials containing the following parts by weight in an aqueous phase and then eluting to remove Ganoderma lucidum polysaccharide template molecules: magnetic metal-organic framework composite carrier: 1.0-1.5 parts; Ganoderma lucidum polysaccharide template molecules: 0.02-0.05 parts; functional monomers: 0.02-0.06 parts. This invention enables the material to directly separate solid and liquid under the action of an external magnetic field after adsorbing Ganoderma lucidum polysaccharides, simplifying the separation operation steps and improving the separation efficiency. Simultaneously, it reduces the interference of monosaccharides, proteins, and other impurities in the extraction system, thereby improving the extraction purity of the target product.

This application proposes a magneto-expandable self-tightening anchor material and its preparation method, comprising the following components by weight: 90-100 parts anchor matrix monomer material, 1 part initiator, 5-8 parts anchor matrix crosslinking agent, 1-2 parts anchor matrix crosslinking promoter, 1-3 parts reinforcing agent, 20-30 parts magnetic molecules, and 3-5 parts interface modifier. The self-tightening anchor material provided by this application only requires the application of an external magnetic field to achieve self-expansion and self-tightening. The addition of an anchoring agent in a random manner reduces the number of steps, reduces costs, and achieves an outstanding reinforcement effect. This application also proposes a preparation method for the self-tightening anchor material, which has the characteristics of simple process, low cost, and easy promotion and application.

The present application belongs to the field of biomedical detection, and particularly relates to a magnetic molecularly imprinted material for detecting various pollutants and a detection method. The present application coats magnetic Fe3O4 material with inorganic SiO2, and then coats the inorganic SiO2 with a layer of organic-inorganic hybrid SiO2 nanoparticles. Through high-temperature treatment, pores are formed on the surface of the organic-inorganic hybrid SiO2 nanoparticles, thereby improving the adsorption of target objects. Then, the SiO2@Fe3O4 material with good dispersity is obtained through activation and dispersion treatment. Finally, a molecular imprinting layer is introduced on the surface of the modified magnetic SiO2@Fe3O4 material to form the magnetic molecularly imprinted material. The material is combined with solid-phase extraction technology to realize the extraction of pollutants in urine. Then, the pollutants are detected through high-performance liquid chromatography. The method effectively reduces the interference of impurities and improves the accuracy of quantification, so that the detection of trace pollutants is more sensitive.

This invention discloses a magnetic molecularly imprinted polymer, its preparation method, and its applications. First, Fe3O4 nanoparticles are prepared via co-precipitation. Then, the Fe3O4 nanoparticles are surface-modified with tetraethyl orthosilicate and 3-(trimethoxysilyl)propyl methacrylate to obtain Fe3O4@SiO2@CH2=CH2 nanoparticles. Using these as the magnetic core of the molecularly imprinted polymer, hexabromocyclododecane is used as the template molecule, 4-vinylpyridine as the functional monomer, azobisisobutyronitrile as the initiator, ethylene glycol dimethacrylate as the crosslinking agent, and acetonitrile as the porogen, to prepare the magnetic molecularly imprinted polymer. The magnetic molecularly imprinted polymer prepared by this invention can rapidly separate hexabromocyclododecane under an external magnetic field with good specificity, exhibiting excellent adsorption and desorption effects. It has low preparation cost, can be recycled, and has good application prospects.

This invention discloses the preparation and application of a novel magnetic, environmentally friendly molecularly imprinted material (MIM) with high-efficiency adsorption and photodegradation for organophosphorus pesticides. The MIM comprises a magnetic ferroferric oxide core coated with mesoporous titanium dioxide to form a shell with photoinduced degradation properties. The MIM is further modified with double bonds and polymerized on the surface using methacrylic acid as a functional monomer and dichlorvos and chlorpyrifos as dual template molecules under the action of trimethylolpropane trimethacrylate (TRIM) crosslinker, forming a novel "core-shell" magnetic MIM. The resulting MIM exhibits uniform particle size, excellent dispersibility, broad-spectrum adsorption for organophosphorus pesticides, good resistance to extreme environments, and multiple reusability. The MIM can be used for the rapid enrichment and detection of organophosphorus pesticides, while minimizing the impact of matrix effects on detection. The MIM has high detection speed, covers a wide range of pesticides, and enables high-throughput detection.

The invention discloses a visual detection sensor for mushroom toxins as well as a preparation method and application of the visual detection sensor. The sensor is composed of a magnetic molecularly imprinted polymer MIPs and dye particles preloaded on the magnetic molecularly imprinted polymer MIPs. The MIPs take Fe3O4 coated SiO2 as a magnetic core, and a specific cavity of a mushroom toxin template molecule is imprinted on the surface of the MIPs; the dye particles are metal organic framework materials ZIF-8-NH2 with the surfaces modified with IPPA, and Congo red dye is encapsulated in the dye particles. During detection, target toxin molecules and dye particles are competitively combined with cavities of the MIPs, the dye is replaced into a solution, after magnetic separation, acid is added for cracking to release a large amount of Congo red, and visual and quantitative detection is realized through solution color change or ultraviolet absorption intensity. The high selectivity of molecular imprinting and the signal amplification function of ZIF-8 are combined, and the method has the advantages of being high in sensitivity, good in selectivity, easy and convenient to operate and low in cost and is particularly suitable for on-site rapid screening of the mushroom toxins.

The application relates to an activated sludge-based antibacterial peptide efficient extraction and purification integrated equipment and belongs to the technical field of activated sludge resource recovery. The application provides an activated sludge-based antibacterial peptide efficient extraction and purification integrated equipment, which comprises a pretreatment and impurity removal unit, a low-temperature alkali hydrolysis unit, a temperature control enzyme hydrolysis unit, a primary purification unit, a secondary fractional purification unit, an antibacterial circle online screening unit and a magnetic molecular imprinting nanometer material enrichment and elution unit which are sequentially connected. The application provides an activated sludge-based antibacterial peptide efficient extraction and purification integrated equipment which is stable in process, safe in preservation, suitable for nanometer Fe3O4@MIPs, and capable of continuous operation.

The application discloses a preparation method of a visual magnetic molecular imprinting fluorescent sensor and application thereof, and discloses the following technical scheme: a sol-gel method is used to imprint on the surface of silicon functionalized ferroferric oxide nanoparticles, eugenol is used as a template molecule, Si-CDs emitting blue fluorescence and CdTe QDs emitting red fluorescence are embedded respectively, and after elution of eugenol, a magnetic molecular imprinting fluorescent sensor with specific recognition sites for eugenol is obtained; by adjusting the volume ratio of different color fluorescent sensors, a dual-fluorescent emission magnetic molecular imprinting fluorescent sensor is constructed. The sensor obtained by the preparation method simultaneously uses blue fluorescence and red fluorescence as response signals, the emission ranges under the excitation wavelength of 365 nm are 430-450 nm and 600-615 nm respectively, the fluorescence intensities of the two emission peaks decrease to different degrees with the increase of the eugenol concentration, and the high selectivity, high sensitivity and accurate visual detection of eugenol are realized through the fluorescence intensity ratio and the change of the solution color.

This invention discloses an in-situ preparation method for thin films using strong magnetic fields to control the orientation of organic molecules, as well as the prepared thin films and their applications, belonging to the field of organic semiconductor thin film technology. The preparation method includes the following steps: heating rubrene powder in a vacuum and magnetic field environment to evaporate and deposit it onto an upper substrate, thus obtaining a rubrene thin film. This in-situ preparation method for thin films using strong magnetic fields to control the orientation of organic molecules couples a high-vacuum in-situ preparation system with a superconducting strong magnetic field system. The aim is to overcome molecular thermal fluctuations at a pure gas-solid phase transition interface without solvent interference, thereby achieving precise control of the spatial orientation of weakly magnetic organic molecules. The orientation-controlled organic thin films prepared by the strong magnetic field significantly reduce the ferromagnetic resonance linewidth (Δ). H This increases its charge current density. J c,norm Therefore, the thin film after orientation control has a higher spin-charge conversion efficiency.

The application relates to the technical field of food detection, in particular to a special medical food vitamin B12 enrichment and detection material and method based on magnetic molecular imprinting. The material is a magnetic molecular imprinting polymer (MMIPs), which takes Fe3O4 as a magnetic core, is coated with a SiO2 layer and a KH570 coupling agent in sequence, and is coupled with a vitamin B12 imprinting polymer layer through surface molecular imprinting technology. The imprinting polymer layer is polymerized from a template molecule vitamin B12 (CNcbl), a functional monomer methacrylic acid (MAA) and a crosslinking agent trimethylolpropane trimethacrylate (TRIM). The material of the application realizes specific recognition through molecular imprinting technology, and simplifies a separation step by using superparamagnetism, so that the cost is significantly reduced and the efficiency is improved.

The utility model relates to the technical field of protein detection, and discloses a Fe3O4 (at) AuNPs magnetic molecular imprinting sensor detection system which comprises an electrochemical workstation, a magnetic electrochemical molecular imprinting sensor, a carrier, a container, a metal disc and an alternating electric field generation module, the magnetic electrochemical molecular imprinting sensor comprises a working electrode, a counter electrode and a reference electrode, the working electrode, the counter electrode and the reference electrode are all connected with the electrochemical workstation, the working electrode is partially inserted into the container, the metal disc is arranged at the bottom of the container, and the positive electrode of the alternating electric field generating module is connected with the working electrode. The negative electrode of the alternating electric field generation module is connected with the metal disc, the carrier is arranged at the bottom of the working electrode, the carrier is a Fe3O4 (at) AuNPs carrier, and the Fe3O4 (at) AuNPs carrier is obtained by combining a molecularly imprinted polymer of protein with Fe3O4 and then combining with AuNPs. According to the utility model, the detection speed, the detection range and the detection effect of protein are improved.

The invention discloses a molecularly imprinted magnetic inverse opal photonic crystal microsphere capable of specifically recognizing zearalenone as well as a preparation method and application of the molecularly imprinted magnetic inverse opal photonic crystal microsphere. A false template similar to the zearalenone in structure is introduced into the microsphere, and the magnetic inverse opal photonic crystal microsphere is used as a substrate; a molecular imprinting layer capable of specifically recognizing zearalenone is modified on the surface of the microsphere. The microspheres prepared by the invention have very high imprinting factors on ZEN, and the enrichment specificity of ZEN is greatly improved. The novel magnetic molecular imprinting photonic crystal microsphere based on the inverse opal structure is synthesized through a false template strategy and a surface molecular imprinting technology, the novel magnetic molecular imprinting photonic crystal microsphere shows unique technical advantages in the field of ZEN separation and detection, and efficient enrichment of target toxin ZEN is achieved through the selective adsorption characteristic of a periodic pore channel structure; by combining the high separation efficiency of high performance liquid chromatography, an analysis system integrating separation and detection is constructed.

The invention relates to the field of food detection, and discloses a method for rapidly detecting Sudan red in chili powder based on nanoparticles and machine learning, which comprises the following steps: step a, providing a to-be-detected extracting solution obtained from a chili powder sample; step b, carrying out magnetic separation to obtain the magnetic molecularly imprinted polymer loaded with Sudan red I; c, obtaining a Fourier transform infrared spectrum of the magnetic molecularly imprinted polymer loaded with the Sudan red I; step d, extracting multi-dimensional characteristics containing Sudan red I characteristic peak information and magnetic core characteristic peak information in the magnetic molecularly imprinted polymer; step e, learning a regression model through a machine and outputting the concentration of the Sudan red I in the extracting solution to be detected. According to the method, main interferents in a matrix are captured and isolated through magnetic molecules, and a machine learning model is trained through a data set, so that the problems of low accuracy and high omission ratio caused by matrix peak overlapping in a traditional spectral analysis method are solved.

The invention relates to the technical field of analysis and detection, in particular to a detection method of a strong-toxicity new pollutant 6PPD-quinone, which comprises the following steps: carrying out specific adsorption on 6PPD-quinone in a water sample to be detected by adopting a magnetic molecularly imprinted polymer, and eluting to obtain a sample solution enriched with 6PPD-quinone; and detecting the sample solution by adopting a monatomic colorimetric detection method. According to the method, the enrichment of the target object 6PPD-quinone is realized, the pretreatment step is simplified, and both hands of a detector are released; the method has the advantages that high-sensitivity and high-selectivity detection of the content of the 6PPD-content quinone is realized by combining with a monatomic colorimetric detection method, the limitation of large instruments is expected to be broken through, and a brand new solution is provided for on-site, rapid and low-cost monitoring of the ultra-trace 6PPD-quinone in the environment.

The invention discloses a preparation method of a diosmetin magnetic molecularly imprinted covalent organic framework compound specific adsorbent, and relates to preparation of a diosmetin specific adsorbent. The invention aims to solve the technical problems that a traditional magnetic molecularly imprinted material carrier is small in specific surface area, limited in number of recognition sites and not ideal in adsorption capacity, and a natural product diosmetin extraction method is long in extraction / separation time and low in purification efficiency. The method comprises the following steps: synthesizing a functional monomer DMBDU with a ureido structure; the preparation method comprises the following steps: preparing amino functionalized magnetic nanoparticles Fe3O4-NH2; and preparing the diosmetin magnetic molecularly imprinted covalent organic framework compound specific adsorbent. The magnetic ureido covalent organic framework imprinted polymer prepared by the preparation method disclosed by the invention has high specific surface area, strong magnetic response performance and excellent renewability, and shows high-selectivity adsorption and specific recognition on diosmetin. The prepared adsorbent is used for specific adsorption of diosmetin.

This invention belongs to the field of microbial isolation and purification technology, specifically relating to a method for preparing magnetic molecularly imprinted microspheres targeting marine bacteria and their applications. The microspheres use nano-Fe3O4 embedded with SiO2 as a substrate and polydopamine as the imprinting layer, forming a three-dimensional recognition cavity within the imprinting layer that complements the surface morphology and chemical composition of *Roseobacterium spp.* ISM strain. Specifically, nano-Fe3O4 is synthesized via co-precipitation, and a SiO2@Fe3O4 composite substrate is prepared via a sol-gel method. Dopamine self-polymerization imprinting is performed using an intact *Roseobacterium spp.* ISM strain as a template, and the microspheres are obtained after template removal. These microspheres exhibit superparamagnetism, structural stability, and good dispersibility. They can specifically adsorb and rapidly magnetically separate target bacteria from complex marine bacterial communities within 30 minutes. The purified target bacteria achieve a purity of 99.04%–100% without damaging the strain's activity, providing a high-quality bacterial strain foundation for marine sulfur cycle research and ecological restoration.

The invention belongs to the technical field of natural product extraction and purification, and particularly relates to a method for selectively separating astaxanthin by using a molecularly imprinted polymer, which comprises the following steps: (1) preparing a modified magnetic molecularly imprinted polymer; (2) preparing an astaxanthin crude extract; (3) adsorption separation; and (4) desorbing and purifying to obtain a high-purity astaxanthin product. According to the invention, the magnetic ferroferric oxide nanoparticles with the surfaces modified by the silane coupling agent and the titanate coupling agent are taken as a core carrier, so that the mechanical stability and the dispersity of the molecularly imprinted polymer are effectively improved; through the synergistic effect of the magnetic ferroferric oxide nanoparticles subjected to surface dual modification, the methacrylic acid and 4-vinylpyridine composite functional monomer and the modified graphene quantum dots, high-selectivity recognition, rapid magnetic separation and stable recycling performance of the astaxanthin are achieved.