Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing magnetic molecule mark polymer for detecting PDE-5 inhibitor

A PDE-5, imprinting polymer technology, applied in the field of preparation of specific magnetic molecular imprinting polymers, can solve the problems of application limitation of magnetic nanoparticles, loss of magnetic properties of magnetic nanoparticles, etc., achieve easy surface functional design, prevent particles The effect of reunion and simple operation

Inactive Publication Date: 2011-07-20
NANJING MEDICAL UNIV
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Magnetic nanoparticles are easily oxidized and lose their magnetism. Due to their high specific surface energy and the interaction of magnetic dipoles between particles, they have a strong tendency to aggregate in solution, which greatly limits the application of magnetic nanoparticles.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing magnetic molecule mark polymer for detecting PDE-5 inhibitor
  • Method for preparing magnetic molecule mark polymer for detecting PDE-5 inhibitor
  • Method for preparing magnetic molecule mark polymer for detecting PDE-5 inhibitor

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] A preparation method of magnetic molecularly imprinted polymer for detecting PDE-5 inhibitor, comprising preparing Fe by conventional method 3 O 4 @SiO 2 Nanoparticles, the preparation steps are:

[0032] (1) Preparation of surface-grafted magnetic nanoparticles: Fe 3 O 4 @SiO 2 The nanoparticles are dispersed in toluene, a silane coupling agent is added, nitrogen is protected, the reaction is carried out at 120 ° C for 12-24 hours, and the product is ultrasonically washed to obtain magnetic nanoparticles grafted on the surface. The mass-volume ratio of the silane coupling agent to toluene is 0.06. ~0.09:1 mg / ml, the Fe 3 O 4 @SiO 2 The dosage of nanoparticles is 300mg;

[0033] (2) Preparation of core-shell magnetic nanoparticles / PDE-5 inhibitor composite spheres: Dissolve the surface-grafted magnetic nanoparticles obtained in step (1) together with functional monomers and PDE-5 inhibitor template molecules in toluene , the prepolymerization was carried out at...

Embodiment 1

[0042] Example 1: Preparation of Fe 3 O 4 @SiO 2 Nanoparticles

[0043] In a 250ml three-neck flask, add 80ml deoxygenated deionized water, keep the stirring speed at 800rmp / min, and add FeCl under nitrogen protection 2 ·4H 2 O (1.72 g) and FeCl 3 ·6H 2 O (4.72 g), 10 ml of concentrated ammonia water was added dropwise at 85°C, and reacted for 1 h to obtain Fe 3 O 4 Magnetic nanoparticles, washed with deionized water until neutral for later use;

[0044] Take 0.3gFe 3 O 4 Magnetic nanoparticles, dispersed in 50ml of isopropanol and 2ml of deionized water, kept stirring at 200rmp / min, added 2ml of ammonia water, added 5ml of TEOS at a speed of 20μl / min, reacted at room temperature for 12h to obtain Fe 3 O 4 @SiO 2 Nanoparticles; washed with deionized water until neutral for later use;

Embodiment 2

[0045] Example 2: Preparation of Sildenafil Fe 3 O 4 @SiO 2 -MIPs 1

[0046] like figure 1 As shown, 300 mg of the above Fe 3 O 4 @SiO 2 The nanoparticles were dispersed in 50 ml of toluene, 6 ml of γ-methacryloyloxytrimethoxysilane was added, and the reaction was carried out at 120 °C for 12 h under nitrogen protection. magnetic nanoparticles;

[0047] Disperse 40 mg of surface-grafted magnetic nanoparticles, template molecule sildenafil and functional monomer TFMAA in 50 ml of toluene, stir at room temperature for 1 h, then add crosslinking agent TMPTMA and initiator AIBN to make the above functional monomer, template molecule The molar concentrations of sildenafil, cross-linking agent TMPTMA and initiator AIBN in toluene were 20 mmol / L, 2 mmol / L, 20 mmol / L and 1 mmol / L, respectively, under nitrogen protection, the reaction was carried out at 50 °C for 6 h and 60 °C for 24 h. Finally, the temperature was raised to 85 °C for 6 h. Magnetic nanoparticles / sildenafil co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for preparing a magnetic molecule mark polymer for detecting a PDE-5 inhibitor. The method comprises the flowing steps of: preparing Fe3O4.SiO2 nanoparticles; performing surface grafting on the Fe3O4.SiO2 nanoparticles; performing polymerization reaction; and performing template molecule elution. The magnetic molecule mark polymer prepared by the method has specific recognition ability on template molecules and analog of the template molecules, is easy to recover under an external magnetic field, and can be used for monitoring the PDE-5 inhibitor which is illegally added into functional health-care foods and traditional Chinese treatment medicaments.

Description

[0001] technical field [0002] The invention relates to a preparation method of a specific magnetic molecular imprint polymer using a PDE-5 inhibitor as a template. Background technique [0003] Viagra (sildenafil, Sildenafil) is a drug approved by the U.S. FDA in 1998 for the treatment of male sexual dysfunction (ED). Improper use can cause adverse reactions such as headache, indigestion, and decreased color resolution. It is effective for cardiovascular and diabetes The harm to the patient is particularly serious, and there is a danger of causing sudden death. In 2003, the FDA successively approved ED therapeutic drugs Vardenafil (Vardenafil) and Tadalafil (Tadalafil) to go on the market. Hundreds of chemical derivatives of ED have been synthesized. These compounds have not been safety evaluated and clinically verified, and there are serious safety risks. It is an indisputable fact that health care products with illegally added ED drugs and chemical derivatives are floo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08F220/04C08F220/28C08F2/44C08J9/26C08K9/06C08K3/36C08K3/22
Inventor 周学敏丁美娟王舒林瑜辉戴鹏王若禹袁丽华李芸吴晓丽文婷婷
Owner NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com