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Application of magnetic molecular imprinting technique in chiral recognition of microfluidic system

A magnetic molecular imprinting and microfluidic analysis technology, which is applied in the application field of magnetic molecular imprinting technology, can solve the problems of toxic and side effects, low enantiomeric efficacy, no drug efficacy, etc., and achieves simple operation, low cost, and economical operation. effect of time

Inactive Publication Date: 2013-04-10
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003]In recent years, the analysis and detection of the enantiomeric purity and composition of drugs and drug intermediate molecules has received extensive attention, because for chiral drugs, it is often a One isomer has medicinal effect, while its enantiomer has little or no medicinal effect, and some can even cause serious side effects

Method used

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  • Application of magnetic molecular imprinting technique in chiral recognition of microfluidic system
  • Application of magnetic molecular imprinting technique in chiral recognition of microfluidic system
  • Application of magnetic molecular imprinting technique in chiral recognition of microfluidic system

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Fabrication of PDMS chip: use gallium arsenide (GaAs) positive mold (produced by Nanjing 55th Institute of Electronics) as a template to make a cross-shaped PDMS microfluidic chip channel, such as figure 1 shown. The specific production process is as follows: Take a certain amount of PDMS monomer and curing agent according to 10:1 (mass ratio), mix evenly, degas, pour on the GaAs template, and cure at 70 °C for 2 hours. After cooling, peel off the PDMS chip containing the cross-shaped channel from the template, cut it into the desired shape with a blade, and punch holes in the buffer pool, sample pool and sample waste pool with a puncher to form a diameter of 3 mm holes. At the same time, using the smooth glass plate as a template, follow the same steps to prepare a PDMS chip without microchannels as a cover slip. The PDMS chip containing the cross channel and the PDMS cover sheet without the channel were ultrasonically cleaned with secondary water, methanol, and sec...

Embodiment 2

[0053] (1) Synthesis of magnetic Fe by hydrothermal method 3 o 4 Nanoparticles (NPs): 1.35 g FeCl 3 6H 2 O was dissolved in 40 mL of ethylene glycol, and ultrasonically mixed evenly; 3.6 g of anhydrous NaAc and 1.0 g of polyethylene glycol were added to the above solution, ultrasonicated for 20 min, and the mixed solution was transferred to an autoclave at 180 ° C reaction for 6 h, after the autoclave was cooled to room temperature, the product was washed several times with double distilled water, and dissolved in 4 mL of double distilled water to obtain Fe with a concentration of 50.2 mg / mL and an average particle size of 126 nm. 3 o 4 NPs.

[0054] (2) MIP-Fe 3 o 4 Preparation of PDA NPs magnetic molecularly imprinted polymer: 25 mg Fe 3 o 4 NPs and 50 mg of the template molecule D / L-tryptophan were dissolved in 10 mL of 20 mM PBS buffer solution with pH 8.5. After mechanical stirring and mixing, 15 mg of functional monomer dopamine was added to the above solution...

Embodiment 3

[0061] MIP-Fe 3 o 4 Immobilization of PDA NPs Magnetic Molecularly Imprinted Polymers in PDMS Microchannels

[0062] (1) The fabrication process of the PDMS microfluidic chip refers to the steps in Example 1.

[0063] (2) MIP-Fe 3 o 4 Immobilization of PDA NPs magnetic molecularly imprinted polymers in PDMS microchannels: first place a permanent magnet on the upper and lower sides of the PDMS chip microchannel (so that the mutually attracting poles correspond to each other), and the PDMS microchip channel is rinsed with double distilled water for 10 min Finally, the prepared MIP-Fe imprinted cavity with specific recognition of chiral tryptophan was prepared by vacuum pump 3 o 4 The magnetic molecularly imprinted polymer of PDA NPs was continuously pumped into the separation channel for 5 min. Under the action of an external magnetic field, the MIP-Fe 3 o 4 PDA NPs magnetic molecularly imprinted polymers were rapidly and controllably immobilized at designated positions i...

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Abstract

The invention discloses an application of a magnetic molecular imprinting technique in a chiral recognition of a microfluidic system, provides a new method of constructing a polydimethylsiloxane (PDMS) microfluidic chip system with selective recognition reaction by using the magnetic molecular imprinting technique to a chiral material, and belongs to the field of microfluidic chip technique. The new method of constructing the chiral selective PDMS microfluidic system based on the magnetic molecular imprinting technique has a great number of imprinting sites specifically recognizing D / L-tryptophan and can realize selective, quick and efficient separation of the chiral tryptophan. The technique can be applied to the fields such as imprinting, separation and detection of biomacromolecules (protein, polypeptide and nucleotide).

Description

technical field [0001] The invention relates to the application of a magnetic molecular imprinting technology, in particular to the application of a magnetic molecular imprinting technology in chiral recognition of a microfluidic analysis system. Background technique [0002] Molecular recognition plays an important role in biological evolution in nature. Chemists use some natural compounds or synthetic compounds to simulate biological systems for molecular recognition research, which constitutes the prototype of molecular imprinting technology. Since the 1970s, molecular imprinting technology has developed very rapidly, especially after Mosbach et al. published a report on the study of theophylline molecularly imprinted polymers in "Nature" in 1993, molecular imprinting technology has become a research hotspot among scholars at home and abroad. [1] . Molecularly imprinted technology is to immobilize template molecules (target molecules, also known as imprinted molecules) i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 梁汝萍孟祥英邱建丁
Owner NANCHANG UNIV
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