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Construction and application of impedance type electrochemical sensor based on molecularly imprinted polymer with magnetic surface

A technology of surface molecular imprinting and magnetic molecular imprinting, which is applied in the field of electrochemical sensing, can solve the problems of difficult large-scale production, difficulty in entering and exiting recognition sites of template molecules, and low cost. The effect of identifying and improving electron conductivity

Inactive Publication Date: 2014-12-03
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

MIPs have strong mechanical properties, high stability, easy large-scale production and low cost, which overcome the disadvantages of poor stability, high price and difficulty in large-scale production of biomolecules.
The traditional block imprinting method has slow mass transfer rate, low binding capacity, and difficulty in entering and exiting the recognition site of template molecules. In order to overcome these shortcomings, people have prepared molecularly imprinted polymers on the surface of various solid substrates, especially nanomaterials, that is, surface molecularly imprinted polymers.

Method used

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  • Construction and application of impedance type electrochemical sensor based on molecularly imprinted polymer with magnetic surface
  • Construction and application of impedance type electrochemical sensor based on molecularly imprinted polymer with magnetic surface
  • Construction and application of impedance type electrochemical sensor based on molecularly imprinted polymer with magnetic surface

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Experimental program
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Effect test

Embodiment 1

[0021] (1) Fe 3 o 4 Preparation of magnetic nanoparticles: 1.35 g of ferric chloride hexahydrate was dissolved in 40 mL of ethylene glycol solution, and then 1.0 g of polyethylene glycol and 3.6 g of sodium acetate trihydrate were added. After the above mixed solution was ultrasonically stirred for 1 hour, it was transferred to a polytetrafluoroethylene autoclave and heated at 180 oC for 6 hours. The collected product was washed three times with ethanol and ultrapure water, and dried at 60 oC in a vacuum oven. After 12 hours, Fe with a particle size of 80-120 nm can be produced 3 o 4 magnetic nanoparticles;

[0022] (2) if figure 1(A) Shown, the preparation of magnetic molecularly imprinted polymers: the Fe 3 o 4 , dopamine and Hb were dissolved in 20 mL of PBS buffer solution with pH 6.98, and then 2.1 mL of 2% chloroplatinic acid was added, mechanically stirred at 4 °C for 30 minutes, and then stirred at room temperature for 2 hours. The reaction product was collected...

Embodiment 2

[0027] like figure 1 As shown in (B), the construction of resistive electrochemical sensor based on molecularly imprinted polymers on the magnetic surface: the magnetic glassy carbon electrode was sequentially polished with 1.0, 0.3 and 0.05 μm alumina suspensions, and then sequentially washed with 0.1 mol / L Wash with nitric acid, absolute ethanol and ultrapure water, dry the electrode with nitrogen, drop 10 μL magnetic molecular imprinted polymer suspension on the electrode surface, dry it naturally and wash it with secondary water to obtain molecular imprinting based on magnetic surface. Polymer resistive electrochemical sensors.

[0028] Cyclic voltammetry and AC impedance method ( Figure 4 ) to characterize the performance of the sensing interface. Fe 3 o 4 / PDA-PtNPs-Hb / MGCE has a pair of obvious redox peaks in PBS, when Hb is eluted, Fe 3 o 4 The redox peaks of / PDA-PtNPs / MIPs / MGCE disappeared. Depend on Figure 4 Visible, Fe 3 o 4 / PDA-PtNPs-Hb / MGCE has a lar...

Embodiment 3

[0030] Application of resistive electrochemical sensors based on magnetic surface molecularly imprinted polymers: the prepared sensors were placed in solutions containing different concentrations of Hb for 15 minutes, and the electrode surface was washed with 10 mM pH 6.98 phosphate buffer solution, and the electrode surface was washed with 5.0 mM Fe(CN) 6 4- / 3- Measure the AC impedance of the sensor for the electrochemical probe; with the increase of Hb, the Hb bound to the sensing interface increases, and the impedance of the sensor increases ( Figure 5 ). There was a good linear relationship between AC impedance value and Hb concentration in the range of 0.14 μg / mL-2.7 μg / mL, and the detection limit was 0.05 μg / mL (S / N). Fe 3 o 4 / PDA-PtNPs / NIPs / MGCE was soaked in different concentrations of Hb solutions for 15 minutes, and the recorded impedance values ​​did not change much, indicating that the effect of non-specific adsorption was small. The sensor constructed by th...

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Abstract

The invention discloses a construction method of an impedance type electrochemical sensor based on a molecularly imprinted polymer with a magnetic surface and an application of the sensor in protein detection, and belongs to the technical field of electrochemical sensing. The construction method comprises the following steps: synthesizing the molecularly imprinted polymer on the surface of a magnetic nano particle by taking hemoglobin as a template molecule and dopamine as a monomer and by using the self polymerization performance and the good adhesive capacity of the dopamine; eluting the hemoglobin, so as to prepare the molecularly imprinted polymer with the magnetic surface, wherein the molecularly imprinted polymer with the magnetic surface has the good identification effect on the hemoglobin; and fixing the polymer on the surface of a magnetic glassy carbon electrode under the action of a magnetic field, so as to construct a hemoglobin sensing interface based on the molecularly imprinted polymer with the magnetic surface, wherein the hemoglobin sensing interface based on the molecularly imprinted polymer with the magnetic surface is used for hemoglobin detection. The constructed electrochemical sensor based on the molecularly imprinted polymer with the magnetic surface is simple in method, high in hemoglobin detection sensitivity and good in selectivity.

Description

technical field [0001] The invention relates to a construction method of an impedance electrochemical sensor based on a molecularly imprinted polymer on a magnetic surface and its application in protein detection, belonging to the technical field of electrochemical sensing. Background technique [0002] Inspired by the mechanism of antibody formation, people have synthesized biomimetic polymers that are guided by template molecules and cross-linked with functional monomers. Such polymers are called molecularly imprinted polymers (MIPs). MIPs have recognition cavities that match template molecules in terms of spatial structure and functional groups, and can form multiple interaction sites, and can specifically recognize target molecules. MIPs have strong mechanical properties, high stability, easy large-scale production and low cost, which overcome the disadvantages of poor stability, high price and difficulty in large-scale production of biomolecules. The traditional bulk i...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/26
Inventor 邱建丁黄春芳梁汝萍
Owner NANCHANG UNIV
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