Magnetic molecular imprinting electrochemical sensor used for detecting trace sulfadimidine

A magnetic molecular imprinting and sulfamethazine technology, which is applied in the fields of material electrochemical variables, measuring devices, scientific instruments, etc., can solve the problems such as the lack of relevant research on the detection of trace sulfamethazine, and improve the sensitivity and selectivity. , easy to automate effects

Inactive Publication Date: 2016-08-24
ZHAOQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Combining magnetic molecular imprinting technology with sensors can obtain high-sensitivity and high-selectivity biosensors. There have been related reports, but there is no relevant research on the detection of trace amounts of sulfamethazine.

Method used

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  • Magnetic molecular imprinting electrochemical sensor used for detecting trace sulfadimidine
  • Magnetic molecular imprinting electrochemical sensor used for detecting trace sulfadimidine
  • Magnetic molecular imprinting electrochemical sensor used for detecting trace sulfadimidine

Examples

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Effect test

Embodiment 1

[0022] Embodiment 1: The construction of the magnetic molecularly imprinted electrochemical sensor for detecting trace amounts of sulfamethazine according to the present invention is characterized in that the magnetic molecularly imprinted electrochemical sensor is prepared by the following method:

[0023] (1) Processing of magnetic core electrodes:

[0024] Put the magnetic core glassy carbon electrode in 0.2M sulfuric acid to activate for 20min, then use 1.0, 0.3 and 0.05μm α-alumina powder to polish the surface, rinse it with ultrapure water and dry it, then put the electrode on the K at 5mM 3 [Fe(CN) 6 ] solution, a cyclic voltammetry (CV) scan was performed at a rate of 100 mV / s in the potential range of -0.2V to 0.6V until a stable cyclic voltammetry was obtained.

[0025] (2) Modification of core electrodes:

[0026] Weigh 10 mg magnetically reduced graphene Fe 3 o 4 @rGO was ultrasonically dispersed in 4mL water for 10min to form a dispersion. Use a micropipette...

Embodiment 2

[0031] Example 2: Cyclic voltammetry characterization of the magnetic molecularly imprinted electrochemical sensor for detecting trace amounts of sulfamethazine according to the present invention

[0032] Since the imprinted holes in the membrane can serve as channels for electron transfer, using K 3 [Fe(CN) 6 ] as a probe, according to the magnitude of its current to characterize the surface properties of various electrodes. Such as figure 1 shown, different electrodes at 5mM K 3 [Fe(CN) 6 ] The cyclic voltammograms in the solution are significantly different. Curve a is the cyclic voltammogram of the bare magnetic core electrode, and the redox peak is obvious. As shown in curve b, when a layer of Fe is modified on the surface of the magnetic glassy carbon electrode 3 o 4 After the @rGO composite, the peak current increases significantly, which is due to the Fe 3 o 4 The @rGO composite is very conductive, which can promote electron transfer on the electrode surface a...

Embodiment 3

[0033] Example 3: Scanning electron microscopy characterization of different modified electrodes for magnetic molecular imprinting electrochemical sensing of the present invention for detecting trace amounts of sulfamethazine

[0034] The microstructure of different modified electrodes was characterized by scanning electron microscopy. Such as Figure 2a As shown, the electrode surface is smooth after the molecular imprinted membrane is modified on the surface of the electrode, indicating that the imprinting effect is good; rough (see Figure 2b ).

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PUM

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Abstract

The invention relates to a magnetic molecular imprinting electrochemical sensor used for detecting trace sulfadimidine. A magnetic electrode modified by magnetic reduction graphene, dopamine auto-agglutination is adopted, and the sulfadimidine magnetic molecular imprinting electrochemical sensor is prepared. When the concentration of olaquindox is within the range of 10.0 nM-200.0 nM, the rise degree of electrochemical signals has a good linear relation with the concentration of sulfadimidine, and a method detection limit reaches 0.1 nM. The sensor is used for detecting actual samples, and the recovery rate reaches 92.6-101.7%. The sensor improves sensitivity and selectivity of sulfadimidine, and detection of trace sulfadimidine is easy to automate.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and electrochemical sensing and detection, and relates to a magnetic molecular imprinted electrochemical sensor, in particular to a magnetic molecular imprinted electrochemical sensor for detecting trace amounts of sulfamethazine. Background technique [0002] Sulfamethazine (Sulfadimidine, SM2), also known as sulfamethazine and methadrimidine, is systematically named N-(4,6-dimethyl-2-pyrimidinyl)-4-aminobenzenesulfonamide. Sulfamethazine, sulfa drugs are a class of broad-spectrum antibacterial drugs, widely used in the prevention and treatment of animal diseases, especially livestock and poultry diseases. However, excessive intake of sulfamethazine by the human body affects the immune system and urinary system of the human body, destroys the ecological balance of the normal flora of the body, and leads to drug resistance of pathogenic bacteria. Therefore, the detection and co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 汪洪武刘艳清姚夙严子军余健健
Owner ZHAOQING UNIV
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