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Porous single-walled carbon nanotube and preparation and application of modified electrode thereof

A technology of single-walled carbon nanotubes and glassy carbon electrodes, which is applied to the electrochemical variables of materials, material analysis through electromagnetic means, and measurement devices, etc., which can solve the problems of higher detection values ​​than real values, specificity needs to be improved, and radioactive contamination. to save time and reagents, reduce costs, and achieve low detection limits

Active Publication Date: 2017-06-13
南通诺林新科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, there is incomplete connection of DNA in the HPLC-ECD method, which can lead to the problem that the detection value is higher than the true value; the ELISA method has cross-reactions, which may lead to the defect that the detection value is higher than the true value; 32 The specificity of the post-P labeling method needs to be improved, and it is easy to cause radioactive contamination; in the GD-MS method, the DNA base must first undergo a derivatization reaction, and this process easily leads to the formation of by-products, resulting in false positive results

Method used

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  • Porous single-walled carbon nanotube and preparation and application of modified electrode thereof
  • Porous single-walled carbon nanotube and preparation and application of modified electrode thereof
  • Porous single-walled carbon nanotube and preparation and application of modified electrode thereof

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Effect test

Embodiment 1

[0034] Embodiment 1, preparation of porous single-walled carbon nanotubes

[0035] Weigh 0.8g of single-walled carbon nanotubes, add them into 30mL double-distilled water and sonicate for 30 minutes to obtain a carbon nanotube dispersion; dissolve 2.4g of potassium permanganate in 40mL of double-distilled water to prepare a potassium permanganate solution; Then slowly add it to the carbon nanotube dispersion under stirring conditions; stir for 3 hours in the dark; then put the mixed solution in the refrigerator and freeze it at a temperature below 0°C for 12 hours, and the obtained solid is freeze-dried at low pressure to obtain a solid powder .

[0036] Add the solid powder into the porcelain boat, then put it into the tube furnace, under N 2 Under protection, the temperature was raised to 800° C. at a rate of 10° C. / min, and calcined at a high temperature for 3 hours; the temperature was lowered to room temperature to obtain a single-walled carbon nanotube-manganese composi...

Embodiment 2

[0038] Embodiment 2, preparation of the glassy carbon electrode modified by porous single-walled carbon nanotubes

[0039] Glassy carbon electrode pretreatment: the glassy carbon electrode was polished into a mirror surface with 0.30 μm and 0.05 μm aluminum oxide suspensions in turn, and then ultrasonically cleaned with 95% ethanol and double distilled water in sequence, and inserted with 1.0 mM potassium ferricyanide probe molecule in 0.1M potassium chloride electrolyte solution, and a three-electrode system with a glassy carbon electrode as a working electrode, a platinum column as a counter electrode, and a saturated calomel electrode as a reference electrode was used for cyclic voltammetry Scan (the scanning potential window is -0.2~0.8), take it out, rinse it with double distilled water and dry it.

[0040] Preparation of porous single-walled carbon nanotube modified electrode: 7ul of the above-mentioned porous single-walled carbon nanotube (PSWNT) was drip-coated on the ...

Embodiment 3、8

[0041] The detection of embodiment 3, 8-hydroxydeoxyguanosine

[0042] Select the linear sweep voltammetry technique in the technique option of the electrochemical workstation: set the potential window to 0.1V~0.7V. The glassy carbon electrode modified with porous single-walled carbon nanotubes was used as the working electrode, the platinum column was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode to form a three-electrode system, and they were jointly immersed in 8-hydroxydeoxyguanosine ( 8-OHdG) in 0.2M pH=5.0 phosphate buffer solution (PBS) for linear voltammetry scanning, using origin software to plot, and draw the linear scanning voltammetry curve of PSWNT / GCE modified electrode for 8-OHdG.

[0043] From this linear relationship, the concentration of 8-hydroxydeoxyguanosine is Ipa (μA) =-0.6069 – 0.9696 C(μM) (R 2 =0.9993) and Ipa (μA) =-6.2866 – 0.2429 C (μM) (R 2 =0.9975).

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Abstract

The invention provides a preparation method of a porous single-walled carbon nanotube. The preparation method comprises the following steps of: mixing a single-walled carbon nanotube with potassium permanganate; performing high-temperature calcining; and removing manganese nanoparticles embedded into the wall of the single-walled carbon nanotube by using hydrochloric acid to obtain the porous single-walled carbon nanotube. The porous single-walled carbon nanotube has good adsorptivity and biocompatibility and is an excellent electrode modifying material. The porous single-walled carbon nanotube modified electrode has very high sensitivity (the detection limit is 9.96*10<-10>M) on 8-OHdG detection. Compared with other 8-OHdG sensor, the electrode is wide in detection range, low in detection limit, simple in detection process, high in sensitivity and fast and simple. By etching nanoscale holes in the wall of the single-walled carbon nanotube by means of a carbothermic method, a tedious step of purifying the carbon nanotube is cancelled, so that a lot of time is shortened and reagents are saved, the cost is greatly lowered, and industrial production becomes possible.

Description

technical field [0001] The invention relates to the preparation of a porous single-walled carbon nanotube, and also relates to the preparation of a glassy carbon electrode modified by the porous single-walled carbon nanotube and its application in detecting 8-hydroxydeoxyguanosine. Background technique [0002] Single-walled carbon nanotubes (SWNTs) are a new type of one-dimensional carbon-based nanomaterials. Its unique electrical, optical and mechanical properties make it rapidly become a research hotspot in the fields of physics, chemistry and materials science. It has been reported that the performance of carbon nanotubes can be improved by modifying the structure (substrate or edge) of carbon nanotubes, resulting in new carbon nanotube-based materials. In recent years, more and more researchers have begun to pay attention to the potential application of structures in carbon nanotubes, and found that many catalytic reactions, electron transfer and chemical reactivity on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 刘秀辉尚天翼刘一丹郭志盼刘岳麟
Owner 南通诺林新科技有限公司
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