Viral nucleic acid extraction kit and extraction method

Abstract

The invention discloses a virus nucleic acid extraction kit. The virus nucleic acid extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution, an elution solution,protease K and magnetic beads; the lysis solution comprises sodium perchlorate with the concentration of 1.0-5.0 mol/L, Tris-HCl with the concentration of 10-200 mmol/L, EDTA with the concentration of 1-50 mmol/L, and Triton X-100 with the concentration of 50-100 microlitre/milliliter; the first rinsing solution is sodium perchlorate solution with the concentration of 2.0-2.5 mol/L, and the solvent of the sodium perchlorate solution is 70-80% ethyl alcohol; the second rinsing solution comprises DEPC water and absolute ethyl alcohol, wherein the volume ratio of the DEPC water to the absolute ethyl alcohol is (3-4):(6-7); the elution solution is Tris-HCl-DEPC water solution with the concentration of 8-12 mmol/L. The invention further discloses a method for extracting viral nucleic acid by using the extraction kit. Through optimization of the reagent formula, the extraction kit has the advantages of being rapid and efficient and avoiding pollution, a large amount of time and reagent canbe saved, and the extraction efficiency of the virus nucleic acid is improved.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid extraction, and in particular relates to a viral nucleic acid extraction kit and an extraction method. Background technique [0002] After the virus nucleic acid is extracted and purified and identified by PCR amplification, the test results can be used as one of the clinical references. However, due to the existence of exogenous and endogenous nucleases and their relatively stable enzyme activity, it is relatively difficult to extract and purify high-quality viral nucleic acids from peripheral blood. The formula of nucleic acid extraction reagents has a great influence on the quality and yield of nucleic acid. Therefore, the development of efficient, rapid and high yield nucleic acid extraction reagents is a subject of continuous exploration in this field. As an automated nucleic acid extraction method, the magnetic bead method is based on the specific adsorption of magnetic beads on nuclei...

AI Technical Summary

Problems solved by technology

Traditional virus nucleic acid extraction methods are cumbersome and time-consuming, such as boiling and pyrolysis, high-temperature treatment samples are likely to produce aerosols, and there is a risk of polluting the testing environment; silica gel membrane adsorption methods require multiple centrifuges, which is prone to problems such as cross-contamination

However, the current viral nucleic acid extraction kits on the market are mainly used for qualitative screening. In practical applications, due to low sensitivity and other reasons, it is easy to cause missed detection, or quantitative deviation due to incomplete extraction of nucleic acid, which cannot well reflect the true concentration of the sample.

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