2results about How to "Efficient extraction method" patented technology

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

The invention provides a special-shaped bridge frame contour skeleton line extraction method suitable for an offshore converter station electrical specialty. The method comprises the following steps: S1, obtaining a CAD design file of a target electrical specialty; s2, docking CAD analysis service, extracting a bridge layer polyline, and normalizing coordinates by taking the equipment reference point as an original point; s3, preprocessing the line segment primitives to enclose a polygon; s4, filtering the output polygon in the step S3: calculating a difference value between the area of the polygon and the area of the minimum enclosing rectangle of the polygon, and if the difference value is greater than 1% of the area of the minimum enclosing rectangle, determining that the bridge is a special-shaped bridge; and S5, according to the characteristics of consistency and connectivity of the cross section size of the special-shaped bridge in the electrical professional CAD drawing of the offshore converter station, screening associated components connected with the special-shaped bridge, obtaining the cross section width W through the geometrical relationship, and associating the cross section width W to the corresponding special-shaped bridge. According to the invention, the special-shaped bridge and the contour skeleton line thereof can be automatically identified, the geometrical characteristics of the special-shaped bridge can be accurately described, and the digitalization of the special-shaped bridge can be realized in an auxiliary manner.