6results about How to "Efficient extraction method" patented technology

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

The invention provides a special-shaped bridge frame contour skeleton line extraction method suitable for an offshore converter station electrical specialty. The method comprises the following steps: S1, obtaining a CAD design file of a target electrical specialty; s2, docking CAD analysis service, extracting a bridge layer polyline, and normalizing coordinates by taking the equipment reference point as an original point; s3, preprocessing the line segment primitives to enclose a polygon; s4, filtering the output polygon in the step S3: calculating a difference value between the area of the polygon and the area of the minimum enclosing rectangle of the polygon, and if the difference value is greater than 1% of the area of the minimum enclosing rectangle, determining that the bridge is a special-shaped bridge; and S5, according to the characteristics of consistency and connectivity of the cross section size of the special-shaped bridge in the electrical professional CAD drawing of the offshore converter station, screening associated components connected with the special-shaped bridge, obtaining the cross section width W through the geometrical relationship, and associating the cross section width W to the corresponding special-shaped bridge. According to the invention, the special-shaped bridge and the contour skeleton line thereof can be automatically identified, the geometrical characteristics of the special-shaped bridge can be accurately described, and the digitalization of the special-shaped bridge can be realized in an auxiliary manner.

The invention provides a method for automatically extracting polybrominated organic pollutants in a bottom mud sample, which comprises the following steps: step 1, collecting the bottom mud sample, wrapping the bottom mud sample with an aluminum foil, and performing low-temperature cryopreservation; 2, freeze-drying the sample by using a freeze-drying machine, and then sieving by using a metal sieve to remove impurities; step 3, performing parameter and process setting on an automatic sample pretreatment system built in a laboratory; 4, mixing the pretreated bottom mud with diatomite, filling the mixture into a stainless steel extraction pool, placing a filter membrane at the bottom of the pool body, placing the pool body into a sample extraction position of an accelerated solvent extraction instrument, and extracting a sample to obtain a bottom mud extracting solution; step 5, each time one sample is extracted, the obtained extracting solution is automatically transferred to a full-automatic gel purification quantitative concentration system instrument by a mechanical arm to be concentrated and dissolved, and then is transferred and submitted to a gel permeation chromatography by a sample injection needle of the instrument; step 6, concentrating the extracting solution, and eluting and purifying by using gel permeation chromatography; and 7, introducing the concentrated solution purified by the gel permeation chromatography into a concentrator through an internal pipeline, concentrating in two modes of pressure reduction and heating, and finally transferring the concentrated solution into a brown small sample injection bottle through a sample injection needle to obtain an extracting solution of the sample. The invention aims to provide a method for efficiently and quickly extracting brominated organic pollutants in bottom mud, manual operation is less, the sample pretreatment time is shortened, the extraction efficiency is improved, and convenience is provided for subsequent compound analysis and identification.

The invention provides an extraction method of ganoderan, and particularly belongs to the technical field of plant natural product extraction. The invention provides an extraction method of ganoderma lucidum polysaccharide, which comprises the following steps: mixing ganoderma lucidum powder with an ethanol water solution, refluxing, degreasing, carrying out suction filtration to obtain filter residues, and carrying out vacuum drying to obtain degreased ganoderma lucidum powder; and mixing the degreased ganoderma lucidum powder, cellulase and water, performing enzymolysis, performing hot water extraction, performing filtration to obtain filtrate, performing concentration, and performing freeze drying to obtain the ganoderma lucidum polysaccharide. The extraction method disclosed by the invention is efficient and high in polysaccharide extraction rate.

The invention relates to a trichosanthes kirilowii maxim alkaloid extract and an extraction method and application thereof, and belongs to the technical field of trichosanthes kirilowii maxim extract preparation. The extraction method comprises the following steps: performing ultrasonic extraction on trichosanthes peel powder for multiple times by using a sulfuric acid solution, combining extracting solutions obtained by multiple times of extraction, performing primary concentration on the combined extracting solutions, then adjusting the pH value of the extracting solutions obtained by primary concentration to a target pH value, performing extraction for multiple times by using n-butyl alcohol, combining the extracting solutions obtained by multiple times of extraction, performing secondary concentration to obtain paste, and performing spray drying to obtain the trichosanthes peel extract. The trichosanthes kirilowii maxim alkaloid extract is obtained. The content of alkaloid in the trichosanthes kirilowii maxim alkaloid extract is 60-70%, and the trichosanthes kirilowii maxim alkaloid extract contains 20 kinds of alkaloid, has a good anti-atherosclerosis pharmacological action, and can remarkably improve liver lipid deposition, liver lipid metabolism disorder and abnormal blood lipid level of atherosclerosis mice.

The invention discloses a method for preparing myrtle ketone microcapsules. Myrtle is granulated after mechanical wall breaking; the granules are pre-extracted by subcritical butane to separate low polar components, and the residue is collected; the residue is leached with dichloromethane to extract myrtle ketone; the dichloromethane extraction part is subjected to vacuum rapid column chromatography to roughly separate myrtle ketone, and a mixed solvent of n-hexane and ethyl acetate is used as an eluent to collect the myrtle ketone fraction; the myrtle ketone fraction is further subdivided by vacuum rapid column chromatography, and a mixed solvent of n-hexane and dichloromethane is used as an eluent to collect the myrtle ketone fraction. The invention also discloses a method for preparing myrtle ketone microcapsules, which selects zein and maltodextrin as microcapsule wall materials, citric acid as a cross-linking agent, myrtle ketone as a core material, and food-grade monocaprylic glyceride and sucrose fatty acid ester are added as emulsifiers and plasticizers, and a spray dryer is used for spray drying to obtain myrtle ketone microcapsules.