60results about How to "Efficient extraction method" patented technology

The invention relates to a method for separating tungsten and molybdenum from a molybdenum-contained phosphotungstic acid/phosphowolframate solution. The method comprises the following steps that 1, hydrogen peroxide is added into the molybdenum-contained phosphotungstic acid/phosphowolframate solution; 2, a reduction or decomposition reaction is performed for precipitation to obtain tungstic acid, and filtering and washing are performed; 3, further roasting is performed on the obtained tungstic acid to obtain tungsten trioxide, or the tungstic acid is dissolved through ammonium hydroxide to obtain an ammonium tungstate solution, and finally evaporatve crystallization is performed to obtain APT; 4, an extraction agent is adopted for extracting molybdenum in the filtrate, a raffinate is returned back to the phosphotungstic acid/phosphowolframate solution; and 5, reverse extraction with ammonium hydroxide is performed to obtain an ammonium molybdate solution, and acid precipitation is performed to obtain ammonium tetramolybdate. The molybdenum separated out through the method is directly used for preparing the product, the separation effect is good, the operation process is simple and easy to control, and industrial application and popularization are easy.

The invention discloses a method for extracting magnolol and honokiol crude extract with high efficiency, the method is that: the magnolol is dried up and crushed and sieved into 30 mesh, the magnolol is added to edible alcohol twice at the weight proportion of one grams of magnolol powder to 12 to 20ml edible alcohol, the extract temperature is 80 to 90 DEGC, the extract time is 2.0 to 3.0 hours each time, the concentration of edible alcohol is 50 to 70 percent, the two extraction crude filter liquors are combined and sieved into 200-mesh nylon fabric, a high speed centrifuge is used for centrifuging and clarifying and a clear liquid is taken out; the clear liquid at the temperature of 40 to 45 DEG C is carried out vacuum concentration by a rotary evaporator, the edible alcohol solvent is recovered and is frozen and dried in vacuum so as to obtain the crude extract, the extract rate of magnolol and honokiol is obviously higher than that of the prior art, the amounted manufacturing cost is relatively low. The method is harmless to the human body as well as to the environment by adopting edible alcohol for extraction; therefore, the method is particularly safe.

The invention discloses an enzymolysis preparation method of Cannabis sativa seed meal polypeptide with alpha-glucosidase inhibiting activity. The method includes: drying Cannabis sativa seed meal and crushing it into powder, adding water and stirring the mixture evenly, adjusting the pH to 8.0-10.5, adjusting the temperature to 30-80DEG C, performing stirring at a uniform speed for 0.5h-2h, conducting centrifugation and adjusting the pH of the supernatant to 3-6, thus obtaining protein precipitate; adding water to dissolve the protein, with the proportion being 2-7g:100mL, adjusting the pH to 7.0- 9.5, then adding alkaline protease, with the ratio of the protein weight to the enzyme activity being 0.1-5:1000, keeping the temperature at 50-75DEG C, and controlling the time at 1-4h; subjecting the enzymatic hydrolysate to separation and purification through macroporous adsorption resin and a Sephadex column, and carrying out collecting and drying, thus obtaining the alpha-glucosidase inhibiting active peptide powder. The Cannabis sativa seed meal polypeptide provided by the invention adds new sources for alpha-glucosidase inhibiting active substances, realizes full utilization of raw materials, and has the advantages of high product activity, convenient and feasible preparation process, and environmental protection, etc.

The invention relates to an index keyword extraction method and system based on big geological data. The method comprises the following steps of: importing document and performing geological data format conversion, sentence division, word characteristic tagging and position marking; adding a weight coefficient to the segmented document; initially mining and extracting keywords by a keyword mining algorithm; performing weighted operation on the extracted keyword phrases to obtain the comprehensive weight value of each phrase; initially screening according to the sizes of the comprehensive weight values, and reducing the number of the phrases to be determined; matching the phrases with a word library, and finding terms matched with the phrases or relatively-stipulated terms in the word library; performing association degree calculation on the terms and the document, determining the size of the association degree of the terms, and screening again according to the sizes of the association degree; and finally, sequencing the terms according to industrial characteristics, height correlation, features and the like, and determining the order of the keywords. According to the method, file background and relevance can be considered, a vague keyword is abandoned to the greatest extent, the keywords comprehensively reflecting the core content of the document are provided and are sequenced according to a certain logic order, thus, document retrieval is achieved, convenience is provided for the improvement of retrieval efficiency, and the method is a high-efficient index keyword extraction method for the big geological data.

The invention discloses a virus nucleic acid extraction kit. The virus nucleic acid extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution, an elution solution,protease K and magnetic beads; the lysis solution comprises sodium perchlorate with the concentration of 1.0-5.0 mol/L, Tris-HCl with the concentration of 10-200 mmol/L, EDTA with the concentration of 1-50 mmol/L, and Triton X-100 with the concentration of 50-100 microlitre/milliliter; the first rinsing solution is sodium perchlorate solution with the concentration of 2.0-2.5 mol/L, and the solvent of the sodium perchlorate solution is 70-80% ethyl alcohol; the second rinsing solution comprises DEPC water and absolute ethyl alcohol, wherein the volume ratio of the DEPC water to the absolute ethyl alcohol is (3-4):(6-7); the elution solution is Tris-HCl-DEPC water solution with the concentration of 8-12 mmol/L. The invention further discloses a method for extracting viral nucleic acid by using the extraction kit. Through optimization of the reagent formula, the extraction kit has the advantages of being rapid and efficient and avoiding pollution, a large amount of time and reagent canbe saved, and the extraction efficiency of the virus nucleic acid is improved.

The invention discloses an extracting method of mezerein and drug with mezerein, which comprises the following steps: using alcohol to extract gewang; condensing; extracting; chromatographing; eluting; recrystallizing; drying; obtaining the product with receiving rate at 0.014%-0.03% and purity at 97.00%-99.99%. The drug with mezerein is made of 45-55% mezerein, 40-50% microcrystal cellulose and 3-7% magnesium stearate.

The invention discloses a method for preparing collagen through high-pressure enzymolysis, and belongs to the technical field of bioengineering. The method comprises the following steps: dispersing smashed animal skin, bone and scale and a feed liquid into an enzyme solution according to the proportion of 1:(1 to 15), adding a buffer solution, adjusting the pH to be 4-9, conducting enzymolysis at the temperature of 30-70 DEG C and the pressure of 100-700 Mpa, and conducting solid-liquid separation on the enzymolysis product so as to obtain a collagen liquid; conducting concentration and drying on the collagen liquid to obtain collagen powder. According to the extracted collagen, the property and the activity of natural collagen are kept to the maximum limit, and compared with the prior art, the collagen extraction rate is obviously improved, and the enzymolysis time is obviously shortened (as low as 0.1 h), so that the efficiency is improved, the energy consumption is reduced, pollution on the environment in the extraction process is also avoided, and the extraction method is efficient, economical and green.

The invention belongs to the technical field of spica prunellae extraction, and particularly discloses a method for extracting spica prunellae and rosmarinus officinalis acid on the basis of responsesurface processes. The method has the advantages that spica prunellae is extracted by the aid of cellulase enzymatic hydrolysis processes, technological parameters such as the enzymatic hydrolysis temperatures, the enzymatic hydrolysis pH (potential of hydrogen) values, the cellulase usage and the enzymatic hydrolysis time are reasonably determined and selected in extraction procedures, accordingly, the yield of the spica prunellae and rosmarinus officinalis acid can reach 17.69 mg/g at least, toxic organic solvents can be effectively omitted, the purpose of efficiently extracting the spica prunellae and rosmarinus officinalis acid can be achieved, and the method is high in efficiency and is controllable and stable.

The invention provides an extraction method of selenoprotein in cardamine violifolia, the selenoprotein obtained by the extraction method and application of the selenoprotein. The extraction method comprises the following steps of carrying out enzymolysis on the cardamine violifolia by adopting enzymes including cellulase to obtain a cardamine violifolia enzymatic hydrolysate; and concentrating the cardamine violifolia enzymatic hydrolysate, and drying the concentrated cardamine violifolia enzymatic hydrolysate. According to the method disclosed by the invention, the selenoprotein in the cardamine violifolia is extracted through enzymolysis of the enzymes including the cellulase, so that nutritional ingredients in the cardamine violifolia are not damaged, the efficiency of extracting the selenoprotein through enzymolysis is high, the yield of the selenoprotein is relatively high, the protein content in the selenoprotein is high, the content of total selenium is high, and the total selenium is organic selenium; and in addition, the extraction method is simple, efficient, low in cost, environmentally friendly and easy for large-scale industrial production.

The invention relates to a dendrobium polysaccharide extract, and a preparation method thereof. The preparation method comprises following steps: 1, pretreatment, wherein dendrobium raw material is prepared, and is crushed so as to obtain a raw material powder; 2, extraction, wherein the raw material powder is added into water so as to obtain a pre-mixed solution, the pre-mixed solution is subjected to high differential pressure continuous extraction, an obtained extracted solution is collected, the extraction pressure of high differential pressure continuous extraction is controlled to be -0.1 to 50MPa; 3, separation, wherein the extracted solution is subjected to centrifugation, an obtained supernatant is collected and is subjected to microfiltration so as to obtain a microfiltered clearliquid; and 4, condensation, wherein the microfiltered clear liquid is subjected to condensation so as to obtain the dendrobium polysaccharide extract. The preparation method is capable of increasingdendrobium polysaccharide yield with ensured dendrobium extraction efficiency, and is capable of realizing industrialized continous large scale production.

The invention relates to a citrus bergamot essential oil efficient rapid extraction technology. The technology utilizes crushing, ultrasonic treatment and later extraction processes to prepare high-yield citrus bergamot essential oil.

The invention relates to a method for extracting organic oil pollutants in soil. Specific steps of the method includes that soil polluted by the organic oil pollutants is soaked in organic solvent, is ultrasonically extracted at the temperature of 40 DEG C for 20 minutes, then keeps static and is filtered, extracting agent is removed from obtained filtrate, so that extractives are obtained, and frequency of used ultrasonic waves is 40kHz during ultrasonic extraction. The highest extraction efficiency for the pollutants in soil samples can achieve 99%. The method for extracting organic oil pollutants in soil is a new way for ultrasonically extracting the organic pollutants in polluted soil, has the advantages of simplicity, convenience, capability of saving time, high efficiency, low pollution and energy consumption, and the like, and is applicable to be popularized and used as pretreatment technology for polluted soil and sediment samples.

The invention belongs to the technical field of plant extraction, and particularly relates to a method for extracting and purifying alkaloid in plants and an application. The plant alkaloid is obtained through combination of flash extraction with micro-controlled extraction, and extremely short extraction time and a sufficient and complete distilling manner are used, so that the method disclosed by the invention concurrently has the characteristics of being quick and efficient; the extraction rate and the distilling rate of alkaloid are high, the content of obtained active components is high,the content of impurities is low, the operation time is short, the usage quantity of a solvent is low, and a convenient and efficient obtaining way is provided for extraction and purification of effective components in medical and edible plants.

The invention relates to an efficient extraction method of inonotus obliquus total triterpenes and a preparation method of fine powder of the inonotus obliquus total triterpenes. The method comprises the following steps: crushing an inonotus obliquus raw material to obtain inonotus obliquus coarse powder; grinding the inonotus obliquus coarse powder in a liquid-nitrogen low-temperature environment by using a ball mill to obtain inonotus obliquus micropowder; preparing an inonotus obliquus suspension from the inonotus obliquus micropowder; carrying out shearing ultrafine crushing treatment on the inonotus obliquus suspension to obtain an inonotus obliquus ultrafine powder suspension; centrifuging the inonotus obliquus ultrafine powder suspension to obtain a precipitate, and drying the precipitate to obtain inonotus obliquus ultrafine powder; placing the inonotus obliquus ultrafine powder in a steam explosion test device to obtain ultrafine wall-broken powder; adding ethanol into the ultrafine wall-broken powder, and carrying out vacuum combined ultrasonic microwave-assisted extraction to obtain an inonotus obliquus total-triterpene crude extract; and carrying out vacuum concentration to obtain an inonotus obliquus total-triterpene concentrated lixiviate. According to the method, the ethanol dosage and the extraction time are reduced under the same extraction rate, the purity of the obtained triterpenes is high, and the biological activity of the inonotus obliquus total triterpenes is high.

The invention relates to a preparation method of a fish bait for resisting marine pathogenic vibrios. The preparation method comprises the following steps: heating and steaming glutinous rice, adding ethanol with a mass concentration of 30%-70% while the glutinous rice is hot, and controlling a mass volume ratio of the glutinous rice to the ethanol to be (1:0.01)-(1:0.05) g/mL; uniformly mixing and cooling, sequentially adding rape seed cake powder and a fructus corni extracting solution in proportion, and uniformly stirring, chopping and drying to obtain the fish bait; wherein a mass ratio of the glutinous rice to the rape seed cake powder is 1:1 to 1:10, and a mass volume ratio of the glutinous rice to the fructus corni extracting solution is 1:0.1 to 1:10 g/mL. The fructus corni is taken as a raw material, a technology for extracting a fructus corni phenolic acid compound is optimized by adopting a Box-Behnken response surface method, the yield of the fructus corni phenolic acid compound is improved, and an activity of the extract in resisting marine pathogenic bacteria is improved. The prepared fish bait is environmentally friendly, safe, reliable and capable of effectively resisting marine pathogenic bacteria, and has high industrialization value.

The invention belongs to the technical field of extraction methods, and particularly relates to a method for extracting anthocyanin from perilla leaves. The method comprises the following steps: (1) refrigerating the perilla leaves at 0-5 DEG C for 1-2 h; (2) drying the refrigerated perilla leaves for 10-15 min by using hot air at 90-110 DEG C; (3) soaking the perilla leaves dried by the hot air in an aqueous solution at 6-14 DEG C for 10-15 min to obtain an aqueous solution containing the perilla leaves; (4) firstly adding polyethylene glycol into the aqueous solution obtained in the step (3), heating to 30-35 DEG C, then adding a sucrose fatty acid ester/citric acid mixture, and stirring at a high speed for 20-30 min to obtain an extracting solution; and (5) centrifugally separating the extracting solution, taking a supernatant, adding a gel solution into the supernatant, uniformly stirring, and drying at 90-100 DEG C to form a solid. The extraction method is efficient, low in cost, free of toxic reagents, low in energy consumption and easy and convenient to operate.

The invention relates to a method for preparing eugenol from a clove oil plant, specifically to a method for preparing eugenol from clove oil extracted from a syringe plant, and belongs to the biochemical field. The invention relates to a simple, practical and efficient extraction method. Through HPLC detection, the content of the eugenol product extracted by the method reaches more than 98%.

The invention provides a method for preparing an eel swimming bladder collagen-chitosan-composite sodium alginate hydrogel. The optimal conditions for extracting the collagen of the sea eel swimming bladder by the acid method through a single factor test and response surface optimization are as follows: the acetic acid concentration is 0.42 mol/L, the acidolysis time is 21h, and the liquid-material ratio is 58ml/g. The acid-soluble collagen separated from the sea eel swimming bladder can be used as a proper drug carrier system, is a good medicinal biological material, has a unique porous fiber structure, can be used for absorbing a large amount of water and supporting the skin surface, and has important effects on moisturizing and wrinkle resistance; the collagen can be used as a wound excipient or a drug carrier, has a healing promoting effect on a mouse skin wound, and has huge application potential in biomedical engineering and medicine.

The invention relates to the technical field of artificial intelligence, and particularly discloses a triple information extraction method and device, electronic equipment and a storage medium, and the method comprises: performing semantic segmentation on a to-be-extracted text to obtain n words, wherein n is an integer greater than or equal to 1; according to the n words, performing semantic information extraction on the to-be-extracted text to obtain a semantic vector of the to-be-extracted text; determining a main entity word in the n words according to the semantic vector; according to the main entity word and the semantic vector, determining guest entity words corresponding to the main entity word in remaining words, wherein the remaining words are all words except the main entity word in the n words; determining a relation word between the main entity word and the guest entity word according to the main entity word, the guest entity word, the semantic vector and a preset relation database; and taking the main entity words, the guest entity words and the relational words as triple information of the to-be-extracted text.