Bionic chip kidney

Abstract

The invention discloses a bionic chip kidney. The bionic chip kidney is characterized by comprising a lower acrylic plate (1), wherein cover glass (2), a lower PDMS piece (3), an upper PDMS piece (4)and an upper acrylic plate (5) are sequentially arranged above the lower acrylic plate (1), a glomerular podocyte culture room (6) with an opening direction upward is formed on the lower PDMS piece (3), a glomerular endothelial cell culture room (7) with the opening direction downward is formed on the upper PDMS piece (4), cross sections of the glomerular podocyte culture room (6) and the glomerular endothelial cell culture room (7) are circles with equal diameter, openings of the glomerular podocyte culture room (6) and the glomerular endothelial cell culture room (7) are opposite, and a porous polycarbonate membrane layer (8) is also arranged between the two culture rooms.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a bionic chip kidney. Background technique [0002] Diabetic kidney injury is one of the important causes of chronic kidney disease, and the key links of its pathological changes are glomerulosclerosis and renal interstitial fibrosis. Glomerulosclerosis leads to increased leakage of GFB albumin, resulting in massive proteinuria, triggering and aggravating renal interstitial inflammatory response, resulting in renal interstitial fibrosis, which leads to renal failure in patients with diabetic nephropathy. At present, the research and drug development of diabetic nephropathy requires a large number of pathological models for preclinical verification and evaluation. The traditional in vitro model is mainly based on cell culture, which separates each type of cell and cultures it separately, ignoring the integrity of the nephron, and splitting the interaction between cells in the kidney. ...

AI Technical Summary

Problems solved by technology

Mainly manifested in: ①Due to technical limitations, it is impossible to put multiple cells under the same system for research

②The two-dimensional culture model of cell attachment lacks the microstructure and microenvironment of the three-dimensional culture model, and there is a huge difference between the specific function and internal environment of the real kidney

③Static cell culture, intermittent supply of culture medium cannot ensure that the cells are in continuous contact with fresh body fluids, which is inconsistent with the continuous circulation system in the body

Animal models are inherently complex, and animal models of diabetic nephropathy have a long modeling cycle, low success rate, and high cost, involving many ethical and moral issues, and cannot represent human physiological responses to diabetic nephropathy or drug treatment, which may have a negative impact on clinical patients. Adverse effects of treatment and recovery

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