54 results about "Polycarbonate membranes" patented technology

A polycarbonate electrolyte comprising a polycarbonate membrane matrix and a lithium salt-containing electrolytic solution impregnated into the polycarbonate membrane matrix. The polycarbonate has the formula:

The invention discloses a PM2.5 particulate mass sampler with four sampling channels: in the first sampling channel, the sampling head includes a PM2.5 cutter, a diffused solution container, Teflon filter membrane and nylon filter membrane; the sampling head in the second sampling channel includes a PM2.5 cutter and Teflon filter membrane; the sampling head in the third sampling channel includes PM2.5 cutter and two-layer quartz membrane; the sampling head in the forth sampling channel includes PM2.5 cutter and polycarbonate membrane.

The invention discloses a method for controlled release of active small molecules based on liposomes and an application thereof. The liposomes wrapped with the active small molecules are prepared by afilm dispersion method, and the method includes the following steps: (1) dissolving the active small molecules and lipids in a mixture of methanol and chloroform (1:1, v/v); (2) removing methanol andchloroform by a rotary vacuum evaporation instrument, and then hydrating a lipid membrane with a hydrating liquid; carrying out water bath ultrasonic treatment to obtain liposomes wrapped with the active small molecules, and extruding by a porous polycarbonate membrane; and dialyzing overnight; (3) adding a dopamine solution to a biomaterial, and gently shaking overnight evenly; and carrying outultrasonic cleaning in sterile distilled water; and (4) adding the liposomes loaded with the active small molecules, and carrying out static reaction; and then washing with a sterile phosphate buffersolution, and thus obtaining the surface of a culture plate modified by the liposomes with the small active molecules. In the method, the active small molecule liposomes are covalently grafted on thesurface of polystyrene, and the liposomes are used as a drug delivery carrier to realize controlled release of drugs.

The invention discloses an organic-inorganic compound mesoporous membrane and a preparation method and an application thereof, belonging to the field of membrane materials. The preparation method thereof includes three steps, namely the preparation of precursor solution, the filtration preparation of compound mesoporous membrane and the elution of surface active agent in the membrane, namely that the method of filtration is mainly used for assembling inorganic mesoporous materials in pores in a polycarbonate membrane so as to prepare the compound membrane, and the mode of extraction and elution is utilized for eliminating the surface active agent in the compound membrane, thereby obtaining the organic-inorganic compound mesoporous membrane; and the pore diameter of the mesoporous material in the membrane is 3.0nm or 8.0 nm. Compared with the formerly reported inorganic compound mesoporous membrane, the organic-inorganic compound mesoporous membrane has better flexibility, hard breakage and better acid and alkali resistance. Besides, due to the ordering and the nanometer size effect of the pore diameter of the mesoporous structure, the organic-inorganic compound mesoporous membrane can be applied to the fields of molecular dimension selective transfer, membrane separation, etc.

In order to prepare the unilamellar low-density lipid vesicles, a presolution of vegetable lipids is first prepared; a suitable quantity of lipids, between 4 and 12 mg/ml, mixed with pure water, heated and stirred for about 30 minutes; the suspension obtained is kept at 50-60° C. for one hour and stirred for 2 minutes every 20 minutes. The high molecular weight hyaluronan is added to the lipidic suspension until a final concentration of 1 mg/ml is obtained, and the suspension of lipids and hyaluronan is emulsified by sonication, pumped using a homogenising valve and immediately made to go through a polycarbonate membrane, to obtain a final suspension with 70% of the particles in suspension having a size between 5 and 15 nm, which makes it possible to use the monolayer vesicles of HA and LDL for the cryopreservation and culture of different mammalian cells, specially germ cells.

The invention discloses a preparation method of a polymeric micelle nanofiltration membrane and application thereof, which belongs to the field of porous material separation membranes. The polymer membrane is mainly attached to a macroporous substrate, and a polycarbonate membrane is chosen as the substrate layer. The preparation method of the micelle membrane comprises the following steps: a polymer with an appropriate block proportion and molecular weight is chosen to be dissolved in selective solvent, so that a micelle solution with a specific morphology is formed; the micelle solution is uniformly dispersed, spread onto the macroporous substrate by the vacuum filtration technology and naturally dried, and thereby the block copolymer separation membrane prepared by the dilute micelle solution is obtained. The voids formed between the piled micelles serve as part of membrane pores, thus playing the role of selective separation, while the macroporous substrate of the lower layer helpsincrease the separation efficiency of the membrane. Most of the current methods for preparing polymer membranes are complex and consume a great deal of energy. The membrane production method is universal and simple, the needed cost is low, and moreover, the produced membrane has high separation efficiency, and can be applied to the separation of different sizes of gold nanoparticles.

The invention discloses a lipidosome-based method for modifying a PEEK (polyetheretherketone) surface with hexadecadrol/minocycline and an application of the method. Lipidosome loaded with hexadecadrol and minocycline is prepared with a thin film dispersion method and then modifies the PEEK surface. The method comprises following steps: (1), hexadecadrol and lipids are dissolved in a methanol and chloroform mixture (1:1, v/v); (2), methanol and chloroform are removed by a rotary vacuum evaporator, and then a lipid membrane is hydrated by a hydration solution; lipidosome loaded with hexadecadrol is obtained by water bath ultrasonic treatment, and is extruded by a porous polycarbonate membrane; dialysis is performed overnight; (3), lipidosome is mixed with a minocycline storage solution, the mixture is shaken, and lipidosome loaded with hexadecadrol/minocycline is obtained, and is stored for standby application at 4 DEG C after being dialyzed overnight; (4), PEEK implant is put in a dopamine solution for reaction, and ultrasonic cleaning is performed; (5), the PEEK implant is added to a hexadecadrol/minocycline lipidosome solution for soaking and is slightly cleaned, and a product is obtained. The obtained product can promote bone repair, regeneration and integration.

The invention discloses a method of extracting and separating nano-scale precipitates in steel by means of an organic electrolyte. The method includes the steps of: 1) performing constant-voltage electrolysis with a to-be-extracted steel material as an anode and a stainless steel cylinder as a cathode at a constant temperature to prepare an organic electrolyte which contains the nano-scale precipitates in steel; 2) performing magnetic separation to the organic electrolyte and performing primary filtration through a polyvinylidene fluoride membrane and secondary filtration through a polycarbonate membrane to obtain the nano-scale precipitates in steel; and 3) adding the nano-scale precipitates in steel into anhydrous ethanol, in which a surfactant is added, to perform ultrasonic dispersion, thus obtaining a turbid liquid containing the nano-scale precipitates in steel. The method can completely and losslessly extract the nano-scale precipitates in steel and solves a problem that a conventional extraction method is liable to cause agglomeration of the nano-scale precipitates in steel. The method can be used for accurately detecting the component, size and three-dimensional appearance characters of the nano-scale precipitates in steel.

The invention provides a preparation method for a three-dimensional Pd-Ni nanowire array catalyst for catalyzing electro-reduction of hydrogen peroxide. The method comprises the following steps of brushing liquid low-melting-point bismuth-base alloy on the surface of a track-etching polycarbonate membrane template; carrying out electrochemical deposition in an electrolyte for four hours with a composite electrode as a working electrode, an electrolytic nickel sheet as an auxiliary electrode and a saturated Ag/AgCl electrode as a reference electrode; after the electrochemical deposition process ends, utilizing dichloromethane to dissolve the template and washing through ethyl alcohol to obtain a three-dimensional Ni nanowire array electrode; and utilizing a chemical in-situ growth method to replace the obtained Ni nanowire array electrode in PdCl2 solution to obtain a three-dimensional Pd-Ni nanowire array electrode. According to the preparation method, the manufacturing cost of the catalyst is greatly reduced. A binder and a conductive agent are not needed, so that the utilization rate of the catalyst is improved while the catalyst has high catalytic activity, and a problem of poor cathode activity of a hydrogen peroxide based fuel cell is solved.

The invention discloses a preparation method of an implanted magnetic control drug microchip, which has the steps of: firstly, preparing a polylactic acid drug chip with a drug storage pool by a polydimethylsiloxane convex plate, adding drugs into the drug storage pool and covering ferroferric oxide nanometer particles with high purity on the drugs, and finally, sealing the drug storage pool by a nuclear pore polycarbonate membrane. The preparation method has the advantages of simple operation, easy implementation and low cost. After the obtained polylactic acid chip with drugs is implanted in a human body, the active control of the release action of the drugs can be really realized by the magnetic property of ferroferric oxide and the release of the drugs in fixed point, time and ration of the added drugs through pulse. The invention has wide application prospect in organizational project and the field of the slow release of drugs.

The invention belongs to the technical field of circulating tumor cell detection. The invention particularly discloses a circulating tumor cell detection device based on a hollow nano needle tube electroporation system. The cell detection device comprises a cell culture tank, wherein the cell culture tank comprises a polydimethylsiloxane lump and a polycarbonate membrane, the polydimethylsiloxanelump is provided with a central hole, the polycarbonate membrane corresponds to the bottom of the central hole and is provided with a hollow nano needle tube array, and a cavity formed by the centralhole and the polycarbonate membrane is used for storing cell suspension; the micro-flow channel is used for delivering a drug solution and comprises a PDMS film with a channel and ITO glass, and the PDMS film is adhered to the conductive surface of the ITO glass; and the cell culture tank and the micro-flow channel take uncured PDMS as an adhesion substance, and are integrated and packaged with each other. The cell detection device can well complete in-situ drug regulation and control in a device after CTC recognition, has excellent biocompatibility, and effectively solves the problem of bloodcell background interference in CTC recognition.

The invention relates to a cell membrane particle expressing parafusin and preparation and application of the particle. VSVG is expressed on the cell membrane particle. Biomacromolecules and organelles are wrapped in the cell membrane particle. The cell membrane particle does not have a cell nucleus. The preparation method includes the steps that transient transfection is conducted on Ad293 cells through expression vesicular stomatits virus protein plasmids; the cells are digested through pancreatic enzymes 48 hours after transfection is carried out, and then centrifugation is carried out for 3 min; an obtained cell suspension is placed in a syringe, the syringe is installed in a syringe filter provided with a polycarbonate membrane, the syringe is pushed so that the cell suspension can be pushed from one side of the filter to the other side of the filter, and the cell membrane particle expressing VSVG is obtained. The cell membrane particle expressing parafusin is applied to biomacromolecule and organelle transfer. The membrane particle is larger, can wrap biomacromolecules and mitochondria, and transfers protein and the mitochondria in the aspect of function. Extra chemical reagents are not added, activity of VSVG is effectively maintained, mitochondrion release efficiency of the membrane particle is improved after the membrane particle enters a body, and accordingly mitochondrion transfer efficiency is improved.

The invention provides a lanthanide fluoride two-dimensional nanosheet membrane as well as a preparation method and application thereof, and belongs to the technical field of membrane separation. The preparation method comprises the following steps: mixing water-soluble lanthanide metal salts with a sodium acetate aqueous solution in inert gas to obtain a mixed solution; mixing the mixed solution with a fluorine-containing salt aqueous solution, and carrying out precipitation reaction to obtain a lanthanide fluoride two-dimensional nanosheet suspension; depositing the lanthanide fluoride two-dimensional nanosheet suspensionon a porous substrate, carrying out drying to obtain the lanthanide fluoride two-dimensional nanosheet membrane, wherein the porous substrate is a polyethersulfone membrane, a nylon membrane, a polyvinylidene fluoride membrane, a polycarbonate membrane, a ceramic membrane, a metal membrane or an anodic aluminum oxide membrane. The lanthanide fluoride two-dimensional nanosheet membrane prepared by the invention has the characteristics of high permeability, high separation precision and good stability, and the preparation method is simple; and when the porous substrate is an inorganic membrane, the prepared lanthanide fluoride two-dimensional nanosheet membrane has high solvent resistance and separation performance.

The invention relates to an extracellular vesicle active medicine loading method. The extracellular vesicle active medicine loading method comprises the following steps of performing resuspension on extracellular vesicles with an ammonium sulfate solution, performing ultrasonic treatment with a low-frequency probe, and performing incubation at 4 DEG C for 1h; repeatedly extruding the incubated extracellular vesicles through a polycarbonate membrane, performing PBS dialysis for overnight, and establishing an ammonium sulfate gradient; and performing incubation on the extracellular vesicles after dialysis for overnight and medicines, so as to obtain medicine loading dialysis extracellular vesicles. According to the method disclosed by the embodiment of the invention, the extracellular vesicle active medicine loading method based on ultrasonic treatment through the low-frequency probe and extrusion through the polycarbonate membrane can effectively improve the medicine loading rate of theextracellular vesicles and the medicine stability, is broad in application range.

The invention discloses a preparation method and application of a nano material with a synovitis inhibition function, and belongs to the technical field of biology. According to the key point of the technical scheme, the preparation method comprises the following steps that S1, shRNA-LeptinR is constructed, specifically, a synthesized shRNA-LeptinR fragment is inserted into a plasmid vector to obtain a recombinant plasmid vector, then, the recombinant plasmid vector is transformed into escherichia coli, and the shRNA-LeptinR plasmid is obtained through extraction; S2, a macrophage membrane is prepared; and S3, gene-loaded nanoparticles M2H@RPK is prepared, specifically, the nanoparticles M2H@RPK is prepared by adopting an electrostatic adsorption method, the nanoparticles H@RPK is wrapped with the M2 macrophage membrane extracted in the step S2, and a polycarbonate membrane repeatedly passes through by using a micro extruder to obtain the nanoparticles M2H@RPK. The preparation method is mainly used for relieving the inflammatory cascade reaction in the OA process, and the nano-system targets the cartilage matrix and can effectively relieve joint injury and inhibit the severity of overall arthritis.

The invention discloses an application of an erythrocyte bionic nanomaterial of PCM polypeptide combined with KALA polypeptide in treatment of cardiovascular diseases, and also discloses a preparation method of the erythrocyte bionic nanomaterial. The method comprises the following steps: obtaining a proper quantity of PCM, performing combining with KALA, and performing fusing into a fusion peptide; enabling the fusion peptide to be subjected to amino acid protection operation treatment, so as to obtain PCM/KALA polypeptide; enabling the PCM/KALA polypeptide to be combined on the surface of an erythrocyte membrane (RBCM); and enabling the PCM/KALA polypeptide combined with the erythrocyte membrane to pass through a polycarbonate membrane with the pore diameter of 400nm by utilizing an Extrusion method, so as to construct the RBCM-PCM/KALA erythrocyte bionic nanoparticles. The erythrocyte bionic nanomaterial prepared by the method can be efficiently and rapidly introduced into cells without depending on concentration, and compared with the prior art, the erythrocyte bionic nanomaterial also has a heart enrichment function.

The invention discloses a preparation method of alpha-glucosyl hesperidin modified xanthophyll liposome. The xanthophyll liposome provided by the invention is obtained by modification with alpha-glucosyl hesperidin, and the specific method is as follows: (1) dissolving xanthophyll and a lipid material to obtain a xanthophyll lipid solution; (2) hydrating a lipid membrane, and then performing ultrasonic treatment to obtain a dispersion solution; (3) dropwise adding an alpha-glucosyl hesperidin solution into the dispersion solution, and performing stirring at room temperature overnight; and (4)squeezing the dispersion solution through a polycarbonate membrane to obtain the xanthophyll liposome. The xanthophyll liposome modified by alpha-glucosyl hesperidin has high stability and long-term circulation performance, and significantly improves the absorption of xanthophyll in the eye.

The invention relates to a bionics lung gas-liquid exposing micro fluidic chip administration and cell culture system. The bionics lung gas-liquid exposing micro fluidic chip administration and cell culture system comprises an exposing chamber, a micro fluidic exposing chip, a micro fluidic cell culture chip, and a basal slide glass, wherein a polycarbonate membrane is arranged between the micro fluidic exposing chip and the micro fluidic cell culture chip; an exposing chip communicating pipeline is formed in the micro fluidic exposing chip; a micro fluidic cell culture chip communicating pipeline is formed in the micro fluidic cell culture chip; the exposing chip communicating pipeline in the polycarbonate membrane is used for culturing one kind of cells; and the micro fluidic cell culture chip communicating pipeline in the basal slide glass is used for culturing the other kind of cells. Compared with the prior art, the bionics lung gas-liquid exposing micro fluidic chip administration and cell culture system disclosed by the invention is simpler in integrated operation, on-line monitoring can be realized, and the bionics lung gas-liquid exposing micro fluidic chip administrationand cell culture system has the advantages of being high in reliability and authenticity and the like; besides, a micro fluidic biochip technique is introduced, so that experiment cost and periods arereduced; and the chip pipeline is freely designed, so that the experiment has high freedom degree.

A process for preparing estrogen liposome includes such steps as proportionally dissolving estrogen, lecithin, cholesterol and VE in the organic solvent prepared from methanol and chloroform, rotational evaporating and vacuum sucking to remove organic solvent, hydrating by the buffering phosphate solution to become stable emulsion, high-speed shearing, and press filtering by polycarbonate membrane to obtain the estrogen liposome with low granularity (170 +/- 30 nm).

The invention discloses a preparation method of a bortezomib liposome preparation, which comprises the following steps: by taking hydrogenated soybean lecithin (HSPC), cholesterol and distearic acid phosphatidyl ethanolamine-polyethylene glycol (DSPE-mPEG2000) as raw materials, preparing liposome by an ethanol injection method, extruding the liposome through a polycarbonate membrane filter membrane to reduce the particle size of the liposome, removing ethanol, acetic acid and mannitol through dialysis, and incubating the liposome preparation and bortezomib (BTZ) overnight to obtain the bortezomib liposome preparation. An ethanol injection method is adopted to prepare the liposome, the operation is simple and convenient, two hydroxyl groups of mannitol in the liposome can be subjected to a covalent reaction with boric acid of BTZ, so that the BTZ encapsulation efficiency in the liposome preparation is improved, and the liposome is extruded through a 100 nm polycarbonate film filter membrane to reduce the particle size of the liposome.

The invention belongs to the technical field of membrane filtration, and particularly discloses a construction method and application of an interface catalytic oxidation membrane suitable for algae-water separation. The method comprises the following steps: soaking a polycarbonate membrane in an organic solvent, and continuously soaking the polycarbonate membrane in a mixed aqueous solution of dopamine hydrochloride and polyethyleneimine; after soaking is completed, sequentially soaking the membrane in a polyacrylic acid aqueous solution and a poly (diallyldimethylammonium chloride) aqueous solution and repeating the process with ensuring that the finally used soaking solution is the polyacrylic acid aqueous solution, then soaking the membrane in a ferrous ion solution, then dropwise adding a sodium borohydride aqueous solution to the surface of the membrane, thus producing the interface catalytic oxidation membrane. Nanometer zero-valent iron is loaded on the surface of the interfacecatalytic oxidation film to activate peroxymonosulfate, so that interface catalytic oxidation in the process of filtering algae-containing water is realized. Compared with the traditional in-situ reaction pre-oxidation, the method has the advantages that the rupture rate of algae cells is greatly reduced, so that the water supply safety is better guaranteed.

The invention relates to a preparation method of a double-drug-loading thermosensitive liposome and application thereof. Early diagnosis of tumors can be quickly realized, effective absorption of tumor cells to drugs is increased, the half-life period of the drugs in vivo is prolonged, and bioavailability of the drugs is improved. According to the technical scheme, the preparation method comprises the steps that dipalmitoyl phosphatidylcholine, cholesterol, phospholipid polyethylene glycol-RGD peptide, 2-methoxyestradiol and indocyanine green are weighed in a round-bottom flask, then the mixture is dissolved in a mixed solution of chloroform and methanol to be subjected to ultrasonic dissolution, and a PBS solution is added in the round-bottom flask to be subjected to hydration treatment on a rotary evaporator at room temperature; finally, the double-drug-loading thermosensitive liposome is obtained after polycarbonate membrane extrusion. The preparation method of the double-drug-loading thermosensitive liposome is simple, production and preparation are easy, and the prepared liposome is good in stability, long in drug effect, high in cancer treatment targeting performance, small in adverse reaction, few in drug administration frequency, high in bioavailability and beneficial to being used by patients and is an innovation of the double-drug-loading thermosensitive liposome.