The application discloses a kind of efficient collection and extraction method and application of air eDNA.The method uses
glass fiber filter as sampling filter, uses medium-flow
particulate matter sampler and TSP sampling head to carry out sampling, uses
dry ice to carry out sample preservation transportation, uses PBS buffer and low-temperature low-speed centrifugal
elution DNA, uses PES filter to enrich and precipitate particulate matter, and PES filter is
cut into small pieces to improve
DNA extraction efficiency.The
environmental DNA extracted by the application has higher concentration and good integrity, and the
DNA extracted by the method can obtain good amplification results using universal primers of different species for amplification, which is convenient for subsequent eDNA
macro-
barcode technology to analyze
species diversity.