Preparation method of delivery system of treatment medicine capable of crossing blood brain barrier and specifically targeting brain glioma

A technology for glioma and blood-brain barrier, applied in the field of medicine, can solve the problems of low survival rate, 5-year survival rate of less than 10%, poor targeting, etc., and achieve the effect of improving the treatment effect

Active Publication Date: 2021-05-21
SHENZHEN INST OF ADVANCED TECH
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AI Technical Summary

Problems solved by technology

At present, the standard clinical treatments mainly include surgical resection and chemotherapy. The 2-year survival rate after treatment is less than 30%, the 5-year survival rate is less than 10%, and the median survival time is only 12-15 months.
The main reason is that the BBB prevents most of the therapeutic drugs from entering the glioma tissue and poor drug targeting

Method used

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  • Preparation method of delivery system of treatment medicine capable of crossing blood brain barrier and specifically targeting brain glioma
  • Preparation method of delivery system of treatment medicine capable of crossing blood brain barrier and specifically targeting brain glioma
  • Preparation method of delivery system of treatment medicine capable of crossing blood brain barrier and specifically targeting brain glioma

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Embodiment 1

[0032]1) Preparation of NK cell membrane: Add the collected NK cells into the cell lysis buffer containing protease inhibitors, and ultrasonically treat them in an ice bath for 20-30min, and centrifuge the broken cells with a maximum centrifugal force of 3500g and a temperature of Centrifuge at 4°C for 10 minutes to collect the supernatant, then centrifuge the supernatant at a maximum centrifugal force of 20,000g and a temperature of 4°C for 25 minutes, collect the supernatant, and then put the supernatant at a maximum centrifugal force of 100,000g at a temperature of 4°C Centrifuge at 4°C for 50 minutes, and the precipitates obtained are NK cell membranes (NKCMs);

[0033] 2) Weigh 10 mg of 1,2-distearoyl-sn-glyceroyl-3-phosphoethanolamine-N-[amino(polyethylene glycol) 2000] (ammonium salt) and 2 mg of AIEgens photothermal agent molecule into 5 mL of pure Mix in water, and then sonicate for 5 minutes to obtain liposomal nanoparticles (D-NPs) loaded with photothermal agent mol...

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Abstract

The invention discloses a preparation method of a delivery system of a treatment medicine capable of crossing a blood brain barrier and specifically targeting brain glioma. The preparation method comprises the following steps: step 1) preparing an NK cell membrane: adding NK cells into a cell lysis buffer solution containing a protease inhibitor, and performing ultrasonic centrifugal treatment to obtain the NK cell membrane; step 2) adding 1, 2-distearoyl-sn-glyceryl-3-phosphoethanolamine-N-[amino (polyethylene glycol) 2000] (ammonium salt) and a medicine into pure water, and performing ultrasonic treatment, so as to obtain liposome nano-particles; and step 3) co-extruding the NK cell membrane and the liposome nano-particles through a polycarbonate membrane with a pore size of 220nm to form an NK cell bionic nano-carrier. The NK cell bionic nano-carrier prepared by the preparation method disclosed by the invention is the delivery system of the treatment medicine which crosses the blood brain barrier and specifically targets the brain glioma.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of a drug delivery system for crossing the blood-brain barrier and specifically targeting brain glioma. Background technique [0002] Blood Brain Barrier (BBB) ​​refers to the barrier between plasma and brain cells formed by brain capillary walls and glial cells, and the barrier between plasma and cerebrospinal fluid formed by choroid plexus. These substances pass from the blood into the brain tissue. A variety of solutes in the blood enter the brain tissue from the brain capillaries, which are difficult and easy; some pass through quickly, some slowly, and some cannot pass through at all. [0003] The blood-brain barrier is a general term for the three barriers of blood-brain, blood-cerebrospinal fluid and cerebrospinal fluid-brain. Brain capillaries lack the pores that normal capillaries have, or the pores are few and small. Endothelial cells overlap each other a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K45/00A61K41/00A61K49/00A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K9/127
CPCA61K47/6901A61K45/00B82Y5/00B82Y30/00B82Y40/00A61P35/00A61K41/0052A61K49/0013A61K49/0097A61K9/127A61K41/00A61K47/69A61K49/00A61K47/42
Inventor 蔡林涛邓冠军孙枝红龚萍
Owner SHENZHEN INST OF ADVANCED TECH
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