Multicellular co-culture dish and application method thereof

Abstract

The invention provides a multicellular co-culture dish and an application method thereof. The co-culture dish comprises a casing, clamping grooves and baffles, wherein the casing is divided into a specific cell cavity and more than 3 peripheral cavities by the baffles and the clamping grooves; the peripheral cavities surround the periphery of the specific cell cavity; the baffles on the periphery of the specific cell cavity are detachable baffles; and the baffles among the peripheral cavities are fixed baffles. Membranes are arranged beside the baffles and are made of polycarbonate membranes, and the baffles are made of plastic or glass. The multicellular co-culture dish has a unique model, can be used for researching the single action and synergistic action of a plurality of cells to certain specific target cell, can be applied among a plurality of cells to research the interrelation among the cells, can be used for researching the single action of drugs in Chinese herbal compound and the compatibility action of assistant and guide, can be used for the research and development of new medicine, and can also be used for monitoring the interaction of drugs, so as to reduce toxic and side effects.

Description

[0001] technical field [0002] The invention relates to the field of microbial cell culture devices, in particular to a multi-cell co-culture dish and a use method thereof. [0003] Background technique [0004] At present, it is the development trend of in vitro cell culture to create a growth environment similar to that in vivo, to promote cell proliferation, differentiation, and to present similar in vivo tissue structure and functional traits. Although the existing in vitro single-cell culture technology has the advantages of simple culture, easy operation, low cost, and large-scale application, the human body is a complex microenvironment, and the growth, proliferation, and differentiation of cells are not in an independent process. It occurs in the environment of the body, but is connected and interacted with each other through the circulatory system and the endocrine system in the body, so it cannot truly simulate the environment in the body, and the cells lose...

AI Technical Summary

Problems solved by technology

However, this type of culture technology also has many limitations. For example, it is still very different from the in vivo environment and cannot fully reflect the changes in the body; it will take a lot of time and energy to observe the interaction between two cells. and cost; the cell migration co-cultivation using Transwell chamber has the disadvantages that the medium cannot be unified and the observation of cell growth is very inconvenient

Therefore, neither of the currently existing techniques can meet the current experimental needs

[0005]In addition, the human body is a complex environment where various cells interact, but the occurrence of some diseases is often directly related to a certain key cell, such as Osteoclasts are the key cells in bone destruction in rheumatoid arthritis (RA), and monocytes are the precursor cells of osteoclasts. Therefore, when conducting experiments, it is necessary to study which cells exist to induce monocytes to transform into osteoclasts. The possibility of bone cells, which requires monocytes as the research target cells to observe the individual and synergistic effects of other cells (such as synovial fibroblasts, lymphocytes, osteoblasts) on it, the existing multicellular Co-culture dishes can only observe the results of the joint action of these cells, but cannot perform the above research on monocytes

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