Circulating tumor cell detection device based on hollow nano needle tube electroporation system and detection method thereof

Abstract

The invention belongs to the technical field of circulating tumor cell detection. The invention particularly discloses a circulating tumor cell detection device based on a hollow nano needle tube electroporation system. The cell detection device comprises a cell culture tank, wherein the cell culture tank comprises a polydimethylsiloxane lump and a polycarbonate membrane, the polydimethylsiloxanelump is provided with a central hole, the polycarbonate membrane corresponds to the bottom of the central hole and is provided with a hollow nano needle tube array, and a cavity formed by the centralhole and the polycarbonate membrane is used for storing cell suspension; the micro-flow channel is used for delivering a drug solution and comprises a PDMS film with a channel and ITO glass, and the PDMS film is adhered to the conductive surface of the ITO glass; and the cell culture tank and the micro-flow channel take uncured PDMS as an adhesion substance, and are integrated and packaged with each other. The cell detection device can well complete in-situ drug regulation and control in a device after CTC recognition, has excellent biocompatibility, and effectively solves the problem of bloodcell background interference in CTC recognition.

Description

technical field [0001] The invention belongs to the technical field of circulating tumor cell detection, and in particular relates to a circulating tumor cell detection device and a detection method based on a hollow nano-needle electroporation system. Background technique [0002] Most cancers are diagnosed due to the signs and symptoms of patients or through screening. The above two methods are not enough to make an accurate diagnosis. Further diagnosis results require examination of patient tissue samples. The examination methods can be Divided into three categories: (1) Imaging examination; (2) Biopsy diagnosis; (3) Blood examination. [0003] Imaging examinations include X-rays, computerized tomography scans (abbreviated as CT scans) and endoscopic examinations. [0004] First, X-ray inspection produces a two-dimensional image through X-ray radiation. When X-ray radiation passes through the human body, part of the radiation is absorbed by the human body. Different part...

AI Technical Summary

Problems solved by technology

At present, research on subsequent cell biobehavior research and drug regulation applications requires the release of CTCs from the biological interface after the enrichment stage. The traditional method is to release cells with trypsin, but this method will damage the adhesion protein structure on the cell membrane surface. Changes that may affect subsequent cellular behavior

In recent years, a lot of research work has emerged successively to prepare smart responsive interfaces and use different methods to release CTCs from biological interfaces, such as functionalized interface photoisomerization, electrical methods, thermal response, etc., but environmental stimuli may still give CTCs bring about unpredictable changes in cellular behavior, and at the same time, some methods of releasing cells above will destroy the original functionalized interface, making the biological interface irreusable.

Images