83 results about "Polycarbonate membrane" patented technology

This invention provides a copper alloy having excellent stress relaxation resistance, comprising Ni: 0.1 to 3.0% (% by mass; the same shall apply hereinafter), Sn: 0.01 to 3.0%, and P: 0.01 to 0.3% with the balance consisting of copper and unavoidable impurities, characterized in that the content of Ni in an extraction residue after extraction separation on a filter having an opening size of 0.1 [mu]m by an extraction residue method is not more than 40% in terms of the proportion to the content of Ni in the copper alloy. In the extraction residue method, 10 g of the copper alloy is immersed in 300 ml of a methanol solution having an ammonium acetate concentration of 10% by mass. This copper alloy is used as an anode. On the other hand, platinum is used as a cathode. Galvanostatic electrolysis is carried out at a current density of 10 mA/cm<2>. A solution containing this copper alloy dissolved therein is subjected to suction filtration by a polycarbonate membrane filter having an opening size of 0.1 [mu]m, and the insolubles as the residue are separated and extracted on the filter. The content of Ni in the extraction residue is determined by dissolving the insolubles as the residue on the filter in a solution composed of a mixture of aqua regia and water at a ratio of 1 : 1, and then analyzing the solution by ICP emission spectrometry.

The invention discloses a one-dimensional rod-like mesoporous material and a preparation method thereof and an application thereof, and belongs to the field of mesoporous materials. The preparation method comprises four steps including precursor preparation, filtration preparation of composite mesoporous membrane, elution of surfactant in membrane and dissolving of organic porous membrane, namely, mainly using filtration method to assemble inorganic mesoporous material in the holes of polycarbonate membrane to prepare composite membrane, then using extraction and elution to remove the surfactant in the composite membrane so as to obtain organic-inorganic composite mesoporous membrane, and soaking the organic-inorganic composite mesoporous membrane in dichloromethane solution, and obtaining the one-dimensional rod-like mesoporous material after completely dissolving the organic porous membrane. The rod-like material is a one-dimensional mesoporous silicon dioxide rod with a diameter of 200nm, a length of 10 mu m and a sequential hexagonal mesoporous structure belonging to MCM-41 type with an aperture of 3nm or belonging to SBA-41 type with an aperture of 8nm. The obtained one-dimensional rod-like mesoporous material is applied in the fields of biological macromolecule and catalyst adsorption and the like.

The invention provides a chloride modified gold nano-channel membrane for detecting atrazine and a method for detecting the atrazine by using the gold nano-channel membrane. In the invention, polycarbonate membrane is taken as basilar membrane, Au nano-channel membrane with the diameter of 20-50 nm is prepared by using a chemical precipitation method, and chloride ions self-assembles to the pore wall of the gold nano-channel through a gold-chloride covalent bond. The detection method comprises the following steps of: placing the chloride modified gold nano-channel membrane between a sample injection pool and a transmission pool; adding a buffer solution containing atrazine antibody into the sample injection pool; adding a buffer solution into the transmission pool; maintaining a parallel liquid level of the two pools; applying voltage on the two pools and measuring basement currents; adding a buffer solution containing a sample to be tested into the sample injection pool and carrying out a current signal test through a electrochemical workstation, wherein if current undershoot occurs, the sample to be tested contains the atrazine. The invention has the advantages of high detectionefficiency, strong specificity, capability of measuring the atrazine with the concentration lower limit of 1.7*10-10 M, and better application prospect.

The invention discloses a method for controlled release of active small molecules based on liposomes and an application thereof. The liposomes wrapped with the active small molecules are prepared by afilm dispersion method, and the method includes the following steps: (1) dissolving the active small molecules and lipids in a mixture of methanol and chloroform (1:1, v/v); (2) removing methanol andchloroform by a rotary vacuum evaporation instrument, and then hydrating a lipid membrane with a hydrating liquid; carrying out water bath ultrasonic treatment to obtain liposomes wrapped with the active small molecules, and extruding by a porous polycarbonate membrane; and dialyzing overnight; (3) adding a dopamine solution to a biomaterial, and gently shaking overnight evenly; and carrying outultrasonic cleaning in sterile distilled water; and (4) adding the liposomes loaded with the active small molecules, and carrying out static reaction; and then washing with a sterile phosphate buffersolution, and thus obtaining the surface of a culture plate modified by the liposomes with the small active molecules. In the method, the active small molecule liposomes are covalently grafted on thesurface of polystyrene, and the liposomes are used as a drug delivery carrier to realize controlled release of drugs.

The invention discloses a preparation method of a polymeric micelle nanofiltration membrane and application thereof, which belongs to the field of porous material separation membranes. The polymer membrane is mainly attached to a macroporous substrate, and a polycarbonate membrane is chosen as the substrate layer. The preparation method of the micelle membrane comprises the following steps: a polymer with an appropriate block proportion and molecular weight is chosen to be dissolved in selective solvent, so that a micelle solution with a specific morphology is formed; the micelle solution is uniformly dispersed, spread onto the macroporous substrate by the vacuum filtration technology and naturally dried, and thereby the block copolymer separation membrane prepared by the dilute micelle solution is obtained. The voids formed between the piled micelles serve as part of membrane pores, thus playing the role of selective separation, while the macroporous substrate of the lower layer helpsincrease the separation efficiency of the membrane. Most of the current methods for preparing polymer membranes are complex and consume a great deal of energy. The membrane production method is universal and simple, the needed cost is low, and moreover, the produced membrane has high separation efficiency, and can be applied to the separation of different sizes of gold nanoparticles.

The invention discloses a bionic glomerulus chip. The chip is characterized in that a porous polycarbonate membrane layer is included, and an upper chip layer and a lower chip layer are fixed on the two faces of the polycarbonate membrane layer respectively and both made of polydimethylsiloxane; an upper inlet pool, a rectangular upper cell culture room and an upper outlet pool are thoroughly formed in the upper chip layer, a first channel is communicated between the upper inlet pool and the upper cell culture room, a second channel is communicated between the upper outlet pool and the upper cell culture room, the width of the first channel and the width of the upper cell culture room are both H, and the width of the second channel is h, wherein H:h=10:3; a lower inlet pool, a rectangular lower cell culture room and a lower outlet pool are thoroughly formed in the lower chip layer, a third channel is communicated between the lower inlet pool and the lower cell culture room, a fourth channel is communicated between the lower outlet pool and the lower cell culture room, and the width of the lower cell culture room, the width of the third channel, and the width of the fourth channel are all H.

The invention discloses an in-vitro cell contact type co-culture device and a culture operation method thereof. According to the device, a box bottom is arranged at the bottom end of a culture box body; a box cover is arranged at the upper end of the box body; a cell culture tank is arranged in the culture box body; the culture box body is in the shape of a conical gyro, and the diameter at the upper end of the culture box body is larger than the diameter of the bottom of the culture box body; the cell culture tank is barrel-shaped; the upper and lower diameters of the barrel are the same as the diameter of the box bottom of the culture box body; an isolating membrane is fixedly arranged in the middle of the culture tank, and divides the culture tank into an upper culture tank and a lower culture tank. The culture tank is ingeniously divided into the upper culture tank and the lower culture tank by using the polycarbonate membrane and is respectively inoculated on the upper and lower sides, and the culture tank is arranged in the cell culture box after being overturned, so that substantive cell contact type co-culture is realized, and influence of metabolites of one kinds of cells on another kinds of cells and an intercellular effect caused by direct contact are conveniently researched.

Disclosed is a preparation method of the poly pyrrole-polycarbonate composite membrane of the supported Cibacron Blue, which relates to the preparation technique of the affinity membrane used for the separation and purification of the protein, the invention provides the preparation method of the poly pyrrole-polycarbonate composite membrane of the supported Cibacron Blue having good hydrophilic property and excellent biocompatibility. The invention takes the polycarbonate membrane as the basement membrane, the basement membrane is fixed in the middle of the diffusion pool containing double pools after the pretreatment in the Cibacron Blue solution; the oxidant and pyrrole monomer solution are respectively added into each pool of the diffusion pool that contains two pools; the active ligand Cibacron Blue is added into any pool of the diffusion pool with the reaction temperature being controlled between 0-40 DEG C and the reaction time is 0.5-8h; finally the poly pyrrole-polycarbonate composite membrane of the supported active ligand Cibacron Blue can be obtained after the cleaning treatment of the basement membrane when the reaction finishes. The prepared affinity membrane has great chemical durability, hydrophilic property and excellent biocompatibility. The composite membrane can be used for separation and purification of protein.

The invention belongs to the technical field of circulating tumor cell detection. The invention particularly discloses a circulating tumor cell detection device based on a hollow nano needle tube electroporation system. The cell detection device comprises a cell culture tank, wherein the cell culture tank comprises a polydimethylsiloxane lump and a polycarbonate membrane, the polydimethylsiloxanelump is provided with a central hole, the polycarbonate membrane corresponds to the bottom of the central hole and is provided with a hollow nano needle tube array, and a cavity formed by the centralhole and the polycarbonate membrane is used for storing cell suspension; the micro-flow channel is used for delivering a drug solution and comprises a PDMS film with a channel and ITO glass, and the PDMS film is adhered to the conductive surface of the ITO glass; and the cell culture tank and the micro-flow channel take uncured PDMS as an adhesion substance, and are integrated and packaged with each other. The cell detection device can well complete in-situ drug regulation and control in a device after CTC recognition, has excellent biocompatibility, and effectively solves the problem of bloodcell background interference in CTC recognition.

The invention discloses a preparation method of endothelial vesicles of Escherichia coli for anti-tumor drugs with endogenous high expression of nucleic acids. According to the preparation method of the endothelial vesicles of Escherichia coli, a 'tRNA scaffolding means' is adopted so as to stably insert microRNA into a vector; the vector is transformed into Escherichia coli, thereby allowing highendogenous expression of microRNA with cancer-cell-killing effects; epicellular and periplasmic components of the Escherichia coli are removed by using lysozyme, thereby obtaining protoplast of Escherichia coli; the protoplast is filtered by using polycarbonate membrane, thereby having the protoplast broken; and then, purification is carried out by adopting a ultracentrifugation means so as to have endothelial vesicles of the protoplast separated, thereby obtaining the endothelial vesicles of Escherichia coli with high expression of miRNA and low toxicity. The preparation method provided by the invention is easy to operate, low in production cost, feasible for large-scale fermentation and preparation, low in toxicity, and highly efficient. As a novel pharmaceutical carrier, being appliedin anti-tumor drug preparation, the endothelial vesicles of Escherichia coli are capable of significantly inhibiting development of non-small cell lung cancer; and thus, the endothelial vesicles of Escherichia coli have broad application prospects in the field of pharmaceutical carriers.

The invention relates to a preparation method of an ergosterol and gefitinib combined liposome. According to the method, GEF is entrapped by an ammonium sulfate gradient method to prepare the ERG combined GEF compound lipidosome; the specific preparation method comprises the following steps: fully dissolving SPC, Chole and ERG with chloroform, performing spin-drying on a rotary evaporator, and carrying out vacuum drying to be odorless; adding pure water, and hydrating the material on a horizontal shaking table for 20-50 minutes to obtain an intermediate product; putting the intermediate product in an ice bath, and carrying out ultrasonic treatment; and finally, filtering the material by adopting a multi-stage microfiltration membrane in sequence, and finally, carrying out high-pressure extrusion by adopting a polycarbonate membrane with a thickness of 0.1 mu m. The GEF encapsulation efficiency is taken as an index, the preparation process of the ERG/GEF-LIP is optimized through singlefactor and response surface design, the form, particle size distribution, Zeta potential, in-vitro cumulative release degree and peroxide value of the ERG/GEF-LIP are preliminarily investigated, and in-vitro lung cancer resistance preliminary research is carried out on drug-resistant cells PC-9/GR.

The invention provides a lanthanide fluoride two-dimensional nanosheet membrane as well as a preparation method and application thereof, and belongs to the technical field of membrane separation. The preparation method comprises the following steps: mixing water-soluble lanthanide metal salts with a sodium acetate aqueous solution in inert gas to obtain a mixed solution; mixing the mixed solution with a fluorine-containing salt aqueous solution, and carrying out precipitation reaction to obtain a lanthanide fluoride two-dimensional nanosheet suspension; depositing the lanthanide fluoride two-dimensional nanosheet suspensionon a porous substrate, carrying out drying to obtain the lanthanide fluoride two-dimensional nanosheet membrane, wherein the porous substrate is a polyethersulfone membrane, a nylon membrane, a polyvinylidene fluoride membrane, a polycarbonate membrane, a ceramic membrane, a metal membrane or an anodic aluminum oxide membrane. The lanthanide fluoride two-dimensional nanosheet membrane prepared by the invention has the characteristics of high permeability, high separation precision and good stability, and the preparation method is simple; and when the porous substrate is an inorganic membrane, the prepared lanthanide fluoride two-dimensional nanosheet membrane has high solvent resistance and separation performance.

The invention relates to an extracellular vesicle active medicine loading method. The extracellular vesicle active medicine loading method comprises the following steps of performing resuspension on extracellular vesicles with an ammonium sulfate solution, performing ultrasonic treatment with a low-frequency probe, and performing incubation at 4 DEG C for 1h; repeatedly extruding the incubated extracellular vesicles through a polycarbonate membrane, performing PBS dialysis for overnight, and establishing an ammonium sulfate gradient; and performing incubation on the extracellular vesicles after dialysis for overnight and medicines, so as to obtain medicine loading dialysis extracellular vesicles. According to the method disclosed by the embodiment of the invention, the extracellular vesicle active medicine loading method based on ultrasonic treatment through the low-frequency probe and extrusion through the polycarbonate membrane can effectively improve the medicine loading rate of theextracellular vesicles and the medicine stability, is broad in application range.

The invention discloses a preparation method and application of a nano material with a synovitis inhibition function, and belongs to the technical field of biology. According to the key point of the technical scheme, the preparation method comprises the following steps that S1, shRNA-LeptinR is constructed, specifically, a synthesized shRNA-LeptinR fragment is inserted into a plasmid vector to obtain a recombinant plasmid vector, then, the recombinant plasmid vector is transformed into escherichia coli, and the shRNA-LeptinR plasmid is obtained through extraction; S2, a macrophage membrane is prepared; and S3, gene-loaded nanoparticles M2H@RPK is prepared, specifically, the nanoparticles M2H@RPK is prepared by adopting an electrostatic adsorption method, the nanoparticles H@RPK is wrapped with the M2 macrophage membrane extracted in the step S2, and a polycarbonate membrane repeatedly passes through by using a micro extruder to obtain the nanoparticles M2H@RPK. The preparation method is mainly used for relieving the inflammatory cascade reaction in the OA process, and the nano-system targets the cartilage matrix and can effectively relieve joint injury and inhibit the severity of overall arthritis.

The invention relates to a preparation method of mitochondria-targeted tocopherol-oleanolic acid double-drug nanoparticles. The bioavailability and the targeting property of the medicine can be effectively improved. The technical scheme is as follows: the preparation method comprises the following steps of: respectively dissolving an anti-cancer drug, a targeting carrier, lecithin and cholesterolin an organic solvent until the materials are totally dissolved, mixing the dissolved solutions together, carrying out rotary evaporation to remove the organic solvent to obtain a film formed on the wall, drying the film in a vacuum drying oven, thoroughly removing the organic solvent, adding a hydration solvent, magnetically stirring the hydrated product, carrying out ultrasonic treatment, sequentially passing through polycarbonate films with different pore diameters, and carrying out extrusion crushing to obtain the mitochondria-targeted tocopherol-oleanolic acid double-drug nanoparticles. According to the nanoparticle, triphenylphosphine connected to tocopherol is utilized, positive charges carried by triphenylphosphine are easily enriched in mitochondria, so that the effect of mitochondria targeted drug delivery is achieved, and the nanoparticle is an innovation on mitochondria targeted preparations of nanoparticles.

The invention discloses an application of an erythrocyte bionic nanomaterial of PCM polypeptide combined with KALA polypeptide in treatment of cardiovascular diseases, and also discloses a preparation method of the erythrocyte bionic nanomaterial. The method comprises the following steps: obtaining a proper quantity of PCM, performing combining with KALA, and performing fusing into a fusion peptide; enabling the fusion peptide to be subjected to amino acid protection operation treatment, so as to obtain PCM/KALA polypeptide; enabling the PCM/KALA polypeptide to be combined on the surface of an erythrocyte membrane (RBCM); and enabling the PCM/KALA polypeptide combined with the erythrocyte membrane to pass through a polycarbonate membrane with the pore diameter of 400nm by utilizing an Extrusion method, so as to construct the RBCM-PCM/KALA erythrocyte bionic nanoparticles. The erythrocyte bionic nanomaterial prepared by the method can be efficiently and rapidly introduced into cells without depending on concentration, and compared with the prior art, the erythrocyte bionic nanomaterial also has a heart enrichment function.

A toner comprising a toner particle that contains a binder resin and fine particles A at the surface of the toner particle, wherein the fine particles A are organosilicon polymer fine particles, the organosilicon polymer fine particles have a number-average primary particle diameter of 30 nm to 500 nm, the organosilicon polymer fine particles have an attachment index for a polycarbonate film of not more than 3.5, the organosilicon polymer fine particles penetrate into the toner particle in a penetration depth b (nm), and protrude from the toner particle in a protrusion height c (nm), and b and c satisfy 0.05≤b/(b+c)≤0.40.

The invention discloses a preparation method of alpha-glucosyl hesperidin modified xanthophyll liposome. The xanthophyll liposome provided by the invention is obtained by modification with alpha-glucosyl hesperidin, and the specific method is as follows: (1) dissolving xanthophyll and a lipid material to obtain a xanthophyll lipid solution; (2) hydrating a lipid membrane, and then performing ultrasonic treatment to obtain a dispersion solution; (3) dropwise adding an alpha-glucosyl hesperidin solution into the dispersion solution, and performing stirring at room temperature overnight; and (4)squeezing the dispersion solution through a polycarbonate membrane to obtain the xanthophyll liposome. The xanthophyll liposome modified by alpha-glucosyl hesperidin has high stability and long-term circulation performance, and significantly improves the absorption of xanthophyll in the eye.

The invention relates to a preparation method and application of triptolide mitochondrial targeting liposome, which can effectively solve the problems of improving the anti-cancer curative effect of triptolide and reducing toxicity, and the method comprises the following steps: respectively dissolving an anti-cancer drug, a targeting carrier, lecithin and polyethylene glycol by using an organic solvent, uniformly mixing the solutions obtained by respectively dissolving, and carrying out rotary evaporation to remove the organic solvent to obtain a film formed on the vessel wall; drying the film in a vacuum drying oven, further removing the organic solvent, adding a hydration solvent for dissolution, and magnetically stirring for hydration to obtain a hydrate; and carrying out ultrasonic treatment on the hydrate, and extruding and crushing the hydrate through a polycarbonate film with the pore diameter of 1.0-0.1 mu m to obtain the triptolide mitochondrial targeting liposome. According to the present invention, the component compatibility is scientific and reasonable, the preparation method is easy to operate, and the carried positive charges are easily enriched in mitochondria so as to achieve the mitochondrial targeting drug delivery effect, such that the mitochondrial targeting liposome preparation is the innovation of the liposome mitochondrial targeting preparation, and the new way of the tumor treatment drug is developed.

The invention relates to a bionics lung gas-liquid exposing micro fluidic chip administration and cell culture system. The bionics lung gas-liquid exposing micro fluidic chip administration and cell culture system comprises an exposing chamber, a micro fluidic exposing chip, a micro fluidic cell culture chip, and a basal slide glass, wherein a polycarbonate membrane is arranged between the micro fluidic exposing chip and the micro fluidic cell culture chip; an exposing chip communicating pipeline is formed in the micro fluidic exposing chip; a micro fluidic cell culture chip communicating pipeline is formed in the micro fluidic cell culture chip; the exposing chip communicating pipeline in the polycarbonate membrane is used for culturing one kind of cells; and the micro fluidic cell culture chip communicating pipeline in the basal slide glass is used for culturing the other kind of cells. Compared with the prior art, the bionics lung gas-liquid exposing micro fluidic chip administration and cell culture system disclosed by the invention is simpler in integrated operation, on-line monitoring can be realized, and the bionics lung gas-liquid exposing micro fluidic chip administrationand cell culture system has the advantages of being high in reliability and authenticity and the like; besides, a micro fluidic biochip technique is introduced, so that experiment cost and periods arereduced; and the chip pipeline is freely designed, so that the experiment has high freedom degree.