Gold nano-channel membrane for detecting atrazine and application thereof

A technology of gold nanochannel and atrazine, which is applied in the field of molecular level membrane detection technology, can solve the problems of limitation, large sample size, and complicated detection of pesticides, and achieve strong specificity, good application prospects, and high detection efficiency. Effect

Inactive Publication Date: 2010-06-02
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are complex and time-consuming to detect pesticides, the instruments are not easy to car

Method used

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  • Gold nano-channel membrane for detecting atrazine and application thereof
  • Gold nano-channel membrane for detecting atrazine and application thereof
  • Gold nano-channel membrane for detecting atrazine and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 Preparation of gold nanochannel membrane

[0030] With the porous polycarbonate film as the base film, gold was deposited on the 100nm porous polycarbonate (PC) film by chemical deposition. Immerse the PC membrane in anhydrous methanol and oscillate ultrasonically for 5 minutes to wash away the impurities adsorbed on the base membrane; then immerse the cleaned PC membrane in 0.025M SnCl 2 solution for 45min, constantly shake the solution slightly to make the Sn 2+ Uniformly adsorbed on the surface of basement membrane and membrane pores, take it out and rinse with methanol twice; 2 The membrane was immersed in 0.029MAg(NH 3 ) 2 +In the solution for 15 minutes, take it out and wash it with methanol for 3 times, and then wash it with water for 3 times, the immersion concentration is 7.9×10 -3 M sodium gold sulfite deposition solution (pH = 10.00). After electroless gold plating at 4°C for 7 hours, take it out and wash it with water for 4 times, and then...

Embodiment 2

[0032] Modification of embodiment 2 chloride ions

[0033] The prepared Au nanochannel membrane was utilized piranha solution ( ) soak for 5 minutes to activate, take it out and wash it with water three times, and immerse it in 0.01M KCl aqueous solution for 12 hours, the chloride ions are self-assembled on the Au nanochannel membrane through the gold-chlorine bond, after the modification, take the membrane out and wash it with methanol three times, N 2 Blow dry to obtain chlorine-modified gold nanochannels.

Embodiment 3

[0034] The preparation of embodiment 3 atrazine immunogen

[0035] First prepare atrazine-mercaptopropionic acid derivatives (MPAD), and then further prepare atrazine immunogens, see figure 1 .

[0036] Slowly add 50ml of ethanol containing 5.0mM atrazine into 10mL of ethanol containing 0.48M 3-mercaptopropionic acid and 10.8mM KOH under stirring condition, and heat to reflux at 110°C for 6h. The solvent was evaporated under reduced pressure, and the residue was added to 25 mL of 5% (mass fraction) NaHCO 3 aqueous solution, with 10mL CHCl 3 It was washed three times and then acidified to pH 2 with 6M hydrochloric acid to induce immediate precipitation of the resulting derivative.

[0037] The supernatant was decanted, and the vacuum-dried precipitate was re-dissolved with 1 mL of ethanol for further purification, and then vacuum-dried at 37° C. to obtain MPAD crystals. Mix 50 μmol of MPAD, 125 μmol of dicyclohexylcarbodiimide (DCC), 125 μmol of N-hydroxysuccinimide (NHS) a...

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Abstract

The invention provides a chloride modified gold nano-channel membrane for detecting atrazine and a method for detecting the atrazine by using the gold nano-channel membrane. In the invention, polycarbonate membrane is taken as basilar membrane, Au nano-channel membrane with the diameter of 20-50 nm is prepared by using a chemical precipitation method, and chloride ions self-assembles to the pore wall of the gold nano-channel through a gold-chloride covalent bond. The detection method comprises the following steps of: placing the chloride modified gold nano-channel membrane between a sample injection pool and a transmission pool; adding a buffer solution containing atrazine antibody into the sample injection pool; adding a buffer solution into the transmission pool; maintaining a parallel liquid level of the two pools; applying voltage on the two pools and measuring basement currents; adding a buffer solution containing a sample to be tested into the sample injection pool and carrying out a current signal test through a electrochemical workstation, wherein if current undershoot occurs, the sample to be tested contains the atrazine. The invention has the advantages of high detectionefficiency, strong specificity, capability of measuring the atrazine with the concentration lower limit of 1.7*10-10 M, and better application prospect.

Description

technical field [0001] The invention relates to the field of materials and the field of sensing technology, in particular to a molecular-level membrane detection technology based on nano-channel membrane materials. Background technique [0002] In recent years, with the rapid development of nanotechnology, nanotechnology and many current technologies have collided and combined with each other, resulting in many emerging topics and directions. Nanochannel membrane technology combines the advantages of low energy consumption, simple operation, high sensitivity, pollution-free and recyclable membrane detection with the nanoscale manipulation of nanotechnology, and can detect target substances at the molecular level. [0003] In the development of agriculture, pesticides and herbicides play an important role. It is estimated that the annual grain loss due to diseases, insects, and weeds accounts for about half of the world's agricultural production, and the use of pesticides and...

Claims

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Application Information

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IPC IPC(8): G01N27/40G01N27/403G01N33/531
Inventor 黄杉生赵国庆岳增连彭斌
Owner SHANGHAI NORMAL UNIVERSITY
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