Modified zein nano-drug delivery system wrapped by outer membrane vesicles of bacteria as well as preparation method and application of modified zein nano-drug delivery system

The technology of zein and outer membrane vesicles is applied in the directions of pharmaceutical formulations, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., and can solve the problems of toxic effects and uncontrolled drug release, etc. To achieve the effect of good stability, good sustained release characteristics and high safety

Pending Publication Date: 2022-06-07
LIAONING UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the free drug is directly bound to the cell membrane, the toxicity of the drug may affect the cell membrane, resulting in uncontrolled release of the drug

Method used

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  • Modified zein nano-drug delivery system wrapped by outer membrane vesicles of bacteria as well as preparation method and application of modified zein nano-drug delivery system
  • Modified zein nano-drug delivery system wrapped by outer membrane vesicles of bacteria as well as preparation method and application of modified zein nano-drug delivery system
  • Modified zein nano-drug delivery system wrapped by outer membrane vesicles of bacteria as well as preparation method and application of modified zein nano-drug delivery system

Examples

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Embodiment 1

[0028] Example 1 A modified zein nano-drug-loading system wrapped by bacterial outer membrane vesicles

[0029] (1) The modified zein nano-drug loading system wrapped by bacterial outer membrane vesicles, the preparation method includes the following steps:

[0030] 1. Preparation of modified zein drug-loaded nanoparticle solution

[0031] Accurately weigh 12.5 mg of sodium caseinate with an analytical balance, dissolve it in 7 mL of deionized water, and ultrasonically dissolve it completely to obtain a sodium caseinate solution.

[0032] Precisely weigh 3 mg of paclitaxel with an analytical balance and dissolve it in 1.6 mL of absolute ethanol, ultrasonically dissolve it completely, add 0.4 mL of deionized water to make the ethanol concentration 80% (volume), and then add 10 mg of zein, at 80 Hz Frequency ultrasonic for 7min to obtain a mixed solution. Quickly pour 7 mL of sodium caseinate solution into the mixed solution with a rotating speed of 15 r / min, stir for 1 h, and...

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Abstract

The invention discloses a modified zein nano drug delivery system wrapped by bacterial outer membrane vesicles as well as a preparation method and application of the modified zein nano drug delivery system. The medicine is dissolved in absolute ethyl alcohol, deionized water is added after ultrasonic dissolution, zein is added, and ultrasonic treatment is carried out; quickly adding a sodium caseinate solution into the obtained mixed solution while stirring, and stirring to obtain a modified zein drug-loaded nanoparticle solution; the preparation method comprises the following steps: uniformly mixing bacterial outer membrane vesicles with a modified zein drug-loaded nanoparticle solution, extruding the mixed solution for 2-8 times by using high-pressure nitrogen as an extrusion pressure source through a 200nm polycarbonate film, centrifuging the obtained product, and collecting the precipitate, thereby obtaining the bacterial outer membrane vesicle wrapped modified zein drug-loaded nanoparticle system. The nano drug-loading system provided by the invention is narrow in particle size distribution range and good in stability, and can load hydrophobic drugs which can be dissolved in ethanol, so that the solubility of the hydrophobic drugs in water is enhanced, and the stability and bioavailability of the drugs are improved.

Description

technical field [0001] The invention relates to a biomimetic natural biopolymer nano-carrier, in particular to a modified zein nano-drug-carrying system wrapped by bacterial outer membrane vesicles, and a preparation method and application thereof. Background technique [0002] In recent years, interest in protein biopolymers for drug delivery systems has greatly increased. Proteins have amphiphilic properties and can interact well with drugs and solvents. They are one of the ideal materials for the preparation of nano-drug delivery systems, and have great application prospects in the field of biomedicine. The key features that make native proteins potentially efficient nanocarriers are their availability of natural resources, inherent biocompatibility, biodegradability, non-toxicity, and their biological functions. Zein was listed by the FDA as one of the safest biomaterial excipients in 1985. Due to its good safety and biocompatibility, it is currently widely used in drug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/52A61K47/46A61K47/42A61K31/337
CPCA61K9/5192A61K9/5169A61K9/5068A61K31/337Y02A50/30
Inventor 陈立江李建潮褚宇琦王泽雨马艳
Owner LIAONING UNIVERSITY
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