Method for preparing polypyrole-polycarbonate composite membrane fixed and carried cibacron blue

A technology of polycarbonate and Cymbal blue, which is applied in the field of affinity membrane preparation, can solve the problems of lack of active functional groups, etc., and achieve the effects of excellent hydrophilicity, easy preparation and storage, and simple operation

Inactive Publication Date: 2008-01-16
XIAMEN UNIV
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AI Technical Summary

Problems solved by technology

[0004] Polycarbonate membrane is a track microporous membrane, which has the advantages of uniform pore size (pore size and density are easy to control), good flexibility and mechanical strength, and good chemical stability. However, the membrane lacks active functional groups and must be Modified to meet the requirements of affinity separation

Method used

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  • Method for preparing polypyrole-polycarbonate composite membrane fixed and carried cibacron blue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Pretreatment of basement membrane:

[0025] Take a polycarbonate membrane with a pore size of 0.8 μm, a thickness of 20 μm, and a diameter of 25 mm, soak it in a 30-mL aqueous solution of Cymbal blue with a concentration of 0.2 mmol / L for 30 min, and then place the membrane in a filter with 30 mL of 0.2 The mmol / L Cymbal blue solution was suction filtered 5 times.

[0026] 2) Doping of ligands and oxidation deposition of polypyrrole:

[0027] Get the base film 3 processed by step 1 and fix it in the middle of the diffusion cell device with double cells 1 and 2 (see Figure 1), add 30mmol / L ammonium persulfate aqueous solution 30mL in the pool 1 of the diffusion cell; Add 30mL of a mixed solution containing cymbal blue and pyrrole monomer, the concentration of cymbal blue in pool 2 is 0.2mmol / L, and the concentration of pyrrole monomer is 15mmol / L. Reacted at 4°C for 2.5h.

[0028] 3) Membrane cleaning and preservation:

[0029] After the reaction time is set, the ...

Embodiment 2

[0033] 1) Pretreatment of basement membrane:

[0034] Take a polycarbonate membrane with a pore size of 0.2 μm, a thickness of 20 μm, and a diameter of 25 mm, and fix it in the middle of the diffusion cell device, add 30 mL of 0.4 mmol / L Cymbal blue aqueous solution to Pool 1, and let it stand for 2 hours to diffuse the Cymbal blue through the basement membrane.

[0035] 2) Doping of ligands and oxidation deposition of polypyrrole:

[0036] Pour out the aqueous solution of Cymbal blue in step 1, then add 30mL of 300mmol / L ferric chloride aqueous solution to Pool 1; The concentration of balan is 0.4mmol / L, and the concentration of pyrrole monomer is 100mmol / L. React at 0°C for 30 min.

[0037] 3) Membrane cleaning and storage:

[0038] According to the method of Example 1, the polypyrrole-polycarbonate composite membrane immobilized with Cymbal blue was obtained by cleaning and storing.

Embodiment 3

[0040] 1) Pretreatment of basement membrane:

[0041] Take a polycarbonate membrane with a pore size of 3 μm, a thickness of 20 μm, and a diameter of 25 mm, place it in a device that can be sealed and vacuumed, add 30 mL of Cymbal blue aqueous solution with a concentration of 0.6 mmol / L, and use a vacuum pump to reduce the vacuum degree of the device. Keep at 30kPa for 20min.

[0042] 2) Doping of ligands and oxidation deposition of polypyrrole:

[0043]Take out the basement membrane treated in step 1 and fix it in the middle of the diffusion cell device, add 30 mL of aqueous hydrogen peroxide solution with pH 2 into pool 1 of the diffusion cell; The concentration of Cymbal blue in pool 2 is 0.6mmol / L, and the concentration of pyrrole monomer is 15mmol / L. Reaction at 0°C for 4h.

[0044] 3) Membrane cleaning and storage:

[0045] According to the method of Example 1, the polypyrrole-carbonate composite membrane with immobilized Cymbal blue was obtained.

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Abstract

Disclosed is a preparation method of the poly pyrrole-polycarbonate composite membrane of the supported Cibacron Blue, which relates to the preparation technique of the affinity membrane used for the separation and purification of the protein, the invention provides the preparation method of the poly pyrrole-polycarbonate composite membrane of the supported Cibacron Blue having good hydrophilic property and excellent biocompatibility. The invention takes the polycarbonate membrane as the basement membrane, the basement membrane is fixed in the middle of the diffusion pool containing double pools after the pretreatment in the Cibacron Blue solution; the oxidant and pyrrole monomer solution are respectively added into each pool of the diffusion pool that contains two pools; the active ligand Cibacron Blue is added into any pool of the diffusion pool with the reaction temperature being controlled between 0-40 DEG C and the reaction time is 0.5-8h; finally the poly pyrrole-polycarbonate composite membrane of the supported active ligand Cibacron Blue can be obtained after the cleaning treatment of the basement membrane when the reaction finishes. The prepared affinity membrane has great chemical durability, hydrophilic property and excellent biocompatibility. The composite membrane can be used for separation and purification of protein.

Description

technical field [0001] The invention relates to a preparation technology of an affinity membrane used for the separation and purification of proteins, in particular to a preparation method of a polypyrrole-polycarbonate composite membrane immobilized with Simba blue. Background technique [0002] With the development of bioengineering and life sciences, higher and higher requirements are put forward for the purification and separation of biological macromolecules such as proteins, polypeptides, and enzymes. Although traditional separation methods, such as precipitation, crystallization, membrane separation, and chromatography, can improve product purity, in many cases, there are problems such as cumbersome process, low activity recovery rate, and unsatisfactory purification effect. The affinity membrane technology developed in recent years has shown good application prospects in the field of protein separation and purification, and has achieved many practical applications. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D69/12B01D71/50B01D71/82
Inventor 石巍马媛媛葛东涛张其清
Owner XIAMEN UNIV
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