The invention provides a single-tank bionic system device and medicine dissolving solution as well as an evaluation method using the device and the solution. The device mainly consists of a medicine dissolving chamber, a pH adjuster, a microporous filter and a medicine diffusion cell; each 1000ml of a medicine dissolving solution A contains 7.8894g of NaCl, 9.008g of D-Glucose, 0.2797g of CaCl2, 0.0975g of MgSO4, 1.9524g of MES (fatty acid methyl ester sulfonate), 5.0mL of HCL (1mM) and the balance of water; and each 1000ml of a medicine dissolving solution B contains 0.7977g of KCl, 0.1198g of KH2PO4, 7.8894g of NaCl, 0.0965g of NaH2PO4, 9.762g of MES the Tris pH value of which is adjusted at 6.8-7.8 and the balance of water. By utilizing the device and the evaluation method, the release and absorption law of a delayed-release preparation can be evaluated.