Preparation method of ergosterol combined gefitinib lipidosome

Abstract

The invention relates to a preparation method of an ergosterol and gefitinib combined liposome. According to the method, GEF is entrapped by an ammonium sulfate gradient method to prepare the ERG combined GEF compound lipidosome; the specific preparation method comprises the following steps: fully dissolving SPC, Chole and ERG with chloroform, performing spin-drying on a rotary evaporator, and carrying out vacuum drying to be odorless; adding pure water, and hydrating the material on a horizontal shaking table for 20-50 minutes to obtain an intermediate product; putting the intermediate product in an ice bath, and carrying out ultrasonic treatment; and finally, filtering the material by adopting a multi-stage microfiltration membrane in sequence, and finally, carrying out high-pressure extrusion by adopting a polycarbonate membrane with a thickness of 0.1 mu m. The GEF encapsulation efficiency is taken as an index, the preparation process of the ERG/GEF-LIP is optimized through singlefactor and response surface design, the form, particle size distribution, Zeta potential, in-vitro cumulative release degree and peroxide value of the ERG/GEF-LIP are preliminarily investigated, and in-vitro lung cancer resistance preliminary research is carried out on drug-resistant cells PC-9/GR.

Description

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AI Technical Summary

Benefits of technology

This patented technology describes methods for making certain types of molecules called gamma rays that can be used to detect lung cancer or other diseases caused by harmful substances like nitric oxides. These particles are made up from specific materials with special properties such as ammonia sulfuric acid solution. By adjusting these conditions during synthesis, different forms of this material may have unique characteristics. They then form vesicles containing them inside another liquid medium - they will stick out into tiny droplets when exposed to air at room temperature. When released back after exposure to oxygen gas, it becomes visible again indicating how well their effectiveness has been achieved over time.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding effective treatments against lung cancer caused by specific types or stages of its development such as advanced stage lymphatic system tissue metastasis (EMT), small pulmonary arterial hypertension syndrome (SPHOS), chronic obstructive bronchitis/chronic sinusitis, acute respiratory distress syndromes associated with pneumonia, heart failure, stroke, brain damage, spinal cord injury, liver cirrhosis, chest pain, cardiovascular diseases like coronary heart disease, cerebral vascular accidents, strokes, breast cancer, colon cancer, colorectal cancer, melanomas, leukemia, gastrointestinal tract neoplasms, headaches, neurology disorders, Parkinson's Disease, multiple sclerosis, rheuma, glioblastoma, malignant mesothelioma, hepatopathies, renal failures, bone marrow lesions, sarcoma, skin cancer, cervix cancer, urogenital organs including bladder cancer, urinary pelvis cancer, ovarian cancer, esophageal cancer and stomach cancer.

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