50 results about "Nuclear pore" patented technology

The invention discloses a colorectal cancer Septin9 gene methylation detection kit based on a graded anchoring probe, and belongs to the technical field of molecular diagnosis. The graded anchoring probe is composed of a specific capture probe (F1), a precise anchoring probe (R1), a synergistic blocking probe (F2 / R2) and a short chain complementary sequence (S1) which are Septin9 methylation specific probes and can be fixed in a conical pore channel of a nuclear track membrane in a graded manner to form a capture-anchoring-blocking functional chain; during application, a general accumulation body is formed by combining with a Septin9 methylation target, a nuclear track membrane pore channel is blocked, current drop is caused, and detection is realized. Through cooperation of the probe, the nuclear track membrane and the electrochemistry, the problems of insufficient sensitivity, poor stability and poor specificity of traditional detection are solved, and the kit is suitable for ultra-early diagnosis of colorectal cancer.

An assembling device for a nuclear track membrane air-conditioning window relates to the technical field of nuclear track membrane air-conditioning window processing and comprises a base body tray, a circulating conveying line, a base body feeding mechanism, a base body mounting device, a nuclear track membrane cutting device, a pressing plate feeding mechanism, a pressing plate forced mounting mechanism, a transferring mechanism, an outer fastener feeding mechanism, an outer fastener mounting mechanism, a finished product transferring mechanism and a detecting device. According to the assembling equipment for the nuclear track membrane air-conditioning window, mechanical equipment is used for completing installation of a base body, a nuclear track membrane, a pressing plate and an outer fastener, the difficulty that the front face and the back face cannot be accurately screened in the process that an assembled body formed by installing the base body, the nuclear track membrane and the pressing plate is transferred to new equipment is avoided, manual use is reduced, the production efficiency is improved, and the production cost is reduced. The assembly efficiency is improved, and the assembly cost of the air-conditioning window main body and the production cost of the fresh-keeping box are reduced.

The invention belongs to the technical field of electrochemical biosensors, and particularly relates to an electrochemical biosensor based on probe strand displacement reaction. According to the sensor, a capture probe is fixed in a nuclear track membrane and is specifically combined with a detection probe, and when target nucleic acid exists, on the basis of the Toehold mediated strand displacement reaction principle, the target nucleic acid replaces the detection probe, and electrode impedance change is triggered to achieve detection. Particularly, reversible replacement between the target nucleic acid and the detection probe is realized by designing double Toehold structural domains. The electrochemical biosensor disclosed by the invention is simple, convenient, rapid and high in specificity, and a new thought is provided for constructing a high-sensitivity and reusable electrochemical biosensing platform.

The invention discloses an air freight nuclear track membrane humidity regulation and control fresh-keeping system and method, and the system comprises a cargo hold / container module which is an airplane main cargo hold or a container meeting the aviation standard, and is used for accommodating cargoes to be kept fresh and forming a closed microenvironment; the nuclear track membrane regulation and control layer is arranged in a mode of covering the ventilation opening of the cargo hold / container module, being integrated in the side wall structure or forming an independent humidity regulation buffer cabin; the nuclear track membrane regulation and control layer is composed of at least one layer of nuclear track membrane, and the nuclear track membrane is of a micropore structure which is uniform in pore diameter height and is of a straight-through pore channel. By introducing the intelligent molecular sieve, namely the nuclear track membrane, the system realizes dynamic bidirectional accurate regulation and control on the humidity in the aviation confined space. Compared with a traditional passive moisture absorption or complete sealing scheme, the system has the advantages that excessive moisture causing corruption can be actively removed, meanwhile, water molecules can be captured from a relatively dry airborne environment for humidification, and the two problems of moisture condensation and excessive drying are fundamentally solved.

The application discloses a method for directly preparing a nuclear micropore membrane with regularly arranged nuclear micropores based on a large-area AAO transmission film, and belongs to the technical field of nuclear micropore membrane preparation. The method comprises the following steps: taking a large-area through-hole anodic aluminum oxide (AAO) film as an ion transmission template, closely adhering the AAO film to a polymer substrate, and performing one-time vertical high-energy particle irradiation; particles penetrate along the regular channels of the AAO and form a large-area regular irradiation damage array on the substrate, which corresponds to the template; and through sensitization, chemical etching and post-processing, a nuclear micropore membrane with consistent nuclear micropore distances and regular arrangement is directly obtained. The method omits the traditional multiplication, alignment and multiple irradiation steps, is simple in process, high in product yield, and capable of large-area mass production, and is high in regularity of the nuclear micropore array, adjustable in parameters, and significantly improves the application performance of the nuclear micropore membrane in high-end fields such as radiation detection, wave absorption and high-precision separation.

The application discloses a fluorine removal agent and a preparation method thereof, and relates to the technical field of fluorine removal agents, and comprises the following components in parts by weight: 5-10 parts of an aluminum salt, 5-10 parts of an iron salt, 15-20 parts of a rare earth material, sodium citrate and beta-cyclodextrin; the addition amount of the sodium citrate is 5%-10% of the total mass of the aluminum salt, the iron salt and the rare earth material; and the addition amount of the beta-cyclodextrin is 30%-50% of the mass of the sodium citrate. Through the introduction of the synergistic system of sodium citrate and beta-cyclodextrin, the metal ion precipitation sequence is regulated, meanwhile, the beta-cyclodextrin captures competitive anions through molecular recognition and constructs a hydrogen bond network to block interference, and the combination of the microsphere type and the porous particle type further realizes dynamic directional transmission of pollutants and nuclear pore limited crystallization, and the active site distribution is reconstructed at the molecular level, so that a technical scheme with universality, economy and reliability is provided for the treatment of complex fluorine-containing wastewater.

The invention belongs to the field of membrane material preparation, and relates to an irradiation preparation method of a nuclear track membrane with uniformly distributed pores, which comprises the following steps: determining the vertical size of a beam spot according to the size of a beam window; arranging an original nuclear track membrane, and ensuring that the original nuclear track membrane is stretched and tightened; carrying out one-dimensional scanning on the nuclear track membrane original membrane by adopting an ion beam with the determined vertical size; and in the one-dimensional scanning process, the irradiation fluence I0 of the irradiation single scanning period is determined according to the transmission rate v and the transverse scanning frequency f. The transmission rate v and the transverse scanning frequency f are used as main control parameters for coupling, the accuracy of the irradiation fluence I is achieved, meanwhile, the irradiation uniformity is guaranteed, transverse stripes generated by irradiation can be avoided while the target irradiation fluence is achieved, and uniform pore distribution in the nuclear pore membrane irradiation preparation process is achieved.

The invention discloses a nuclear track membrane irradiation and feeding system. The system comprises an irradiation mechanism; the unwinding assembly is arranged on one side of the irradiation mechanism; the winding assembly is arranged on the other side of the irradiation mechanism, and the unwinding assembly and the winding assembly are symmetrically arranged up and down relative to the irradiation mechanism; the first automatic feeding and discharging module is arranged on the side, away from the irradiation mechanism, of the unwinding assembly, and the first automatic feeding and discharging module is configured to place a raw material film roll on the unwinding assembly and take down a tailing film roll from the unwinding assembly; the second automatic feeding and discharging module is arranged on the side, away from the irradiation mechanism, of the winding assembly, and the second automatic feeding and discharging module is configured to take down the finished film roll from the winding assembly and place the empty roll core on the winding assembly. Through the arrangement of the first automatic feeding and discharging module and the second automatic feeding and discharging module, feeding can be conducted on the unwinding assembly, discharging can be conducted on the winding assembly, feeding is more convenient and faster, feeding and discharging are more accurate, the working efficiency is improved, and the working intensity is reduced.

The application belongs to the field of membrane material preparation, and relates to a method for preparing a nuclear pore membrane with uniform pore distribution through irradiation, comprising the following steps: determining the vertical size of a beam spot according to the size of a beam window; setting a nuclear pore membrane original film, ensuring that the nuclear pore membrane original film is stretched and taut; performing one-dimensional scanning on the nuclear pore membrane original film by using an ion beam with the determined vertical size; during the one-dimensional scanning, determining the irradiation fluence of an irradiation single scanning period according to the transmission rate v and the transverse scanning frequency f . I 0 The application couples the transmission rate v and the transverse scanning frequency f as main control parameters, achieves the accuracy of the irradiation fluence I , ensures the irradiation uniformity, avoids the transverse stripes generated by irradiation while achieving the target irradiation fluence, and realizes the uniform pore distribution in the process of preparing the nuclear pore membrane through irradiation.

The present application relates to a kind of recyclable nuclear pore structure MOF nano flame retardant and its preparation method, belong to the cross technical field of nano and flame retardant.The present application provides a kind of nuclear pore structure MOF nano flame retardant, polyurethane foam is soaked in high concentration nano flame retardant, and foam material no longer appears melt drop phenomenon and self-extinguishes from fire, cannot be secondarily ignited, and foam material shows good flame-retardant heat-insulating effect, and smoke suppression effect is outstanding.After being introduced into foam material, the elasticity of foam material itself is improved, in addition, the nuclear pore structure of it gives foam material itself higher sound insulation and noise reduction effect.

The utility model provides a preservation box containing a nuclear track membrane. The preservation box comprises a box body, a cover body used for opening or closing the box body and a preservation mechanism used for controlled atmosphere preservation. The fresh-keeping mechanism comprises an inner window body, an outer window body detachably connected to the inner window body and an air adjusting assembly arranged between the inner window body and the outer window body, the inner window body is used for preventing liquid in the box body from entering the air adjusting assembly, and the outer window body is used for preventing liquid outside the box body from entering the air adjusting assembly; the air adjusting assembly is clamped and fixed through the inner window body and the outer window body. The air adjusting assembly comprises a front dust cover, a rear dust cover detachably connected to the front dust cover and a nuclear track membrane located between the front dust cover and the rear dust cover, and the nuclear track membrane is clamped and fixed through the front dust cover and the rear dust cover. The air conditioning device has the advantages of being stable in air conditioning function, convenient to maintain and suitable for household use.

The application discloses a nuclear pore membrane modified atmosphere packaging device production system, which comprises a mother piece feeding mechanism, a nuclear pore membrane cutting mechanism, a sub piece feeding mechanism and a multiple-speed chain conveying line. The automatic feeding of the mother piece and the sub piece is realized through the mother piece feeding device and the sub piece feeding device, and the automatic cutting of the nuclear pore membrane during the assembling process of the nuclear pore membrane and the sub piece is realized through the nuclear pore membrane cutting device. The overall assembling of the nuclear pore membrane modified atmosphere packaging device is realized to be automatic, the working efficiency of the assembling is obviously improved, and the qualified rate of the finished product is improved.

The invention discloses an electrochemical luminescence biosensor based on bimetallic nano-enzyme and DNA walker and application thereof, and belongs to the technical field of biosensing. The sensor takes a nuclear track membrane as a substrate, gold nanoparticles are loaded on the inner wall of the membrane, thiolated aptamers are designed, the aptamers are fixed on the surfaces of the gold nanoparticles through Au-S bonds, thiolated trigger DNA and Pd (at) Pt nano-enzyme containing gold sites are mixed in PBS and coupled through the Au-S bonds, then the trigger DNA-Pd (at) Pt compound is dispensed on the surface of the aptamer modified membrane, and the sensor is obtained. The preparation method comprises the following steps: preparing Au (at) Ag nano-clusters, hybridizing the Au (at) Ag nano-clusters with aptamers, fixing the Au (at) Ag nano-clusters on the inner wall of a membrane, self-assembling the Au (at) Ag nano-clusters on carboxyl sites on the inner wall of the membrane layer by layer through electrostatic adsorption, fixing Track DNA on the inner wall of the membrane, adding Nt.BbvCI endonuclease, closing non-specific binding sites by using bovine serum albumin, and fixing CDS quantum dots on the other side of the membrane. The electrochemical luminescence biosensor of the bimetallic nano enzyme and the DNA walker is obtained. The sensor has the advantages of high sensitivity, low detection limit, good stability, simple preparation steps, short detection time and the like.

The present application relates to the field of tumor detection, and in particular to a biological sample analysis method and system based on nuclear pore distribution patterns. The method provided by the present application comprises: S101, obtaining microscopic image data of a biological sample; S102, obtaining a first region of interest, a second region of interest and a third region of interest; S103, drawing a nuclear membrane edge curve; S104, extracting nuclear pore distribution-nuclear membrane curvature distribution data of cells in the biological sample; S105, identifying the nuclear pore distribution pattern of the cells in the biological sample; S106, evaluating the state of the tissue or organ corresponding to the biological sample. Based on the identified nuclear pore distribution pattern of individual cells in the biological sample, the present application can evaluate the state of the tissue or organ corresponding to the biological sample according to the overall nuclear pore distribution pattern of the cells in the biological sample, such as whether it is a tumor sample, whether it has a tendency to cancer, etc., which is of great significance for early diagnosis of tumors.

The invention discloses an electrochemical luminescence biosensor based on a functionalized nuclear track membrane. According to the electrochemical luminescence biosensor, a nuclear track membrane is used as a substrate, and terpyridyl ruthenium functionalized silver nanoparticles are modified in nanometer pore channels of the nuclear track membrane and are used as ECL illuminants; a gold nano layer is loaded on the outer surface of the nuclear track membrane through a spin-coating method, and a trialkali matrix consisting of a dopamine aptamer and an auxiliary chain containing a hydrophobic group is fixed. When the dopamine is combined with the main chain of the aptamer, the structure of the trialkali matrix is unwound to release an auxiliary chain, the hydrophobic barrier is eliminated, so that the nano pore channel is converted into an open state from a closed state, and the material in the pore channel is in contact with a co-reactant tripropylamine (TPrA) in the electrolyte to generate a remarkably enhanced ECL signal. The dopamine concentration is in a good linear relationship with the ECL strength in a range of 0.1 nM to 10 mu M (R2 is equal to 0.995). According to the sensor, the interface stability is enhanced through the gold nanolayer, the recognition precision is improved through the aptamer, the pore permeability is dynamically regulated and controlled through the hydrophobic group, and high-selectivity detection of dopamine is achieved.

The present invention provides a photoelectrochemical biosensor based on cascade amplification and its application in ultrasensitive detection of cardiac troponin, belonging to the field of biosensing technology. The present invention uses a nuclear pore membrane containing multiple nanopores as a substrate, and modifies the inner wall of the nanopores of the nuclear pore membrane with Z-scheme heterojunction g-C 3 N 4 @CdS quantum dots. Then, the double-stranded hybrid of aptamer Apt1 and ssDNA1 is sequentially immobilized on the inner wall of the nanopores of the nuclear pore membrane through amidation reaction. Then, ssDNA2-MB is modified on the inner wall of the pore to form a double-lock recognition interface. Then, hairpin DNAs H1 and H2 are respectively modified with Fe 3 O 4 @Pt nanozyme, pre-assembled in the nanopores, and then the non-specific sites are blocked to obtain a photoelectrochemical biosensor based on cascade amplification, realizing ultrasensitive (0.02 fmol / mL) and highly specific detection of cTnI, and at the same time simplifying the operation process and improving the anti-interference performance of the photoelectrochemical biosensor and its application in ultrasensitive detection of cardiac troponin.

The present invention discloses a nuclear pore membrane biochip multiple detection device, including a collection device and a detection device, the collection device includes a first nuclear pore membrane, the first surface of the first nuclear pore membrane faces the body fluid flowing through the first nuclear pore membrane, so as to enrich the cells used for detection in the body fluid on the first surface; the detection device includes a preservation liquid cup containing a preservation liquid, the first surface of the first nuclear pore membrane can be immersed in the preservation liquid cup, and the preservation liquid is used to protect the cell morphology; the second nuclear pore membrane is detachably arranged at the bottom of the preservation liquid cup, and is used to filter the preservation liquid to enrich the cells in the preservation liquid on the first side of the second nuclear pore membrane. The present invention uses the first nuclear pore membrane to enrich the cells to be detected in the body fluid, and transfers them to the preservation liquid for exfoliated cell morphology detection, and uses the second nuclear pore membrane to enrich the cells in the preservation liquid for immunodetection or FISH detection, so as to realize multiple detection in combination with the exfoliated cell morphology detection, and more accurately detect the lesions in the body.

The invention discloses a fluorine removal agent and a preparation method thereof, and relates to the technical field of fluorine removal agents, the fluorine removal agent comprises the following components by weight: 5-10 parts of an aluminum salt, 5-10 parts of an iron salt, 15-20 parts of a rare earth material, sodium citrate and beta-cyclodextrin; the adding amount of the sodium citrate is 5%-10% of the total mass of the aluminum salt, the ferric salt and the rare earth material; the addition amount of the beta-cyclodextrin is 30%-50% of the mass of the sodium citrate; a synergistic system of sodium citrate and beta-cyclodextrin is introduced, the precipitation sequence of metal ions is regulated and controlled, meanwhile, beta-cyclodextrin captures competitive anions through molecular recognition and constructs a hydrogen bond network to block interference, and dynamic directional transmission and nuclear pore confinement crystallization of pollutants are further achieved through combination of a microsphere type and a porous particle type. Active site distribution is reconstructed on the molecular level, and a technical scheme with universality, economical efficiency and reliability is provided for complex fluorine-containing wastewater treatment.

The application provides a kind of nanometer hole nuclear pore membrane rapid nondestructive testing method based on dark field scattering optical method, comprising: using dark field optical microscope to focus on the nuclear pore membrane to be detected, after focusing, adjust to dark field mode, obtain the scattering light image of the nuclear pore membrane to be detected under dark field mode;Determine the image area and the number of scattering light spots in the image based on the scattering light image, obtain the density of the nuclear pore membrane;Move the dark field optical microscope light spot center to the single hole position to collect dark field scattering spectrum, obtain the single hole dark field scattering spectrum intensity I ;Based on the relationship between scattering spectrum intensity I And aperture D Formula I = aD 2 +b , the aperture of the nuclear pore module is calculated by the single hole dark field scattering spectrum intensity I Therefore, the application has the advantages of simple operation, low cost, fast detection speed, etc., and can be widely applied in the field of nanotechnology and material science.

The invention relates to a high-fidelity expansion microscopic imaging method of a nuclear pore complex, which is characterized in that the signal intensity and the dyeing coverage are obviously enhanced through the immunostaining conditions of 37 DEG C temperature and prolonged incubation time, and the permeability of an antibody in iterative expansion gel is improved; by adopting a secondary anchoring cross-linking condition that 2.8% paraformaldehyde and 10% acrylamide are proportioned, under the condition that the expansion rate is not reduced, the structural heterogeneity index is further reduced, and the marking integrity is improved; through treatment of 92.5 mM SDS under the denaturation condition of 85 DEG C for 1.5 h, full denaturation can be achieved, and deformation and signal loss caused by protein breakage can be reduced. During imaging, the signal-to-noise ratio of fluorescence after single expansion is improved by using a water immersion objective lens, so that a clear annular NPC structure can be obtained by using a confocal microscope; therefore, the image fidelity and the signal-to-noise ratio are remarkably improved, and a technical basis is provided for subsequently realizing near-molecule-scale NPC imaging under a confocal microscope.

The invention discloses a self-pressure-relief waterproof loudspeaker with a waterproof and breathable nuclear track vibrating membrane, and belongs to the technical field of loudspeakers. Comprising a loudspeaker support, a nuclear track waterproof breathable vibrating membrane and a loudspeaker surface cover, the nuclear track waterproof breathable vibrating membrane is composed of a nuclear track membrane, one end of the loudspeaker support is provided with a loudspeaker magnetic circuit, loudspeaker coils are arranged inside and outside the loudspeaker magnetic circuit, and the loudspeaker coils are fixedly connected with the nuclear track waterproof breathable vibrating membrane. The loudspeaker surface cover presses the edge of the nuclear track waterproof breathable vibrating membrane on the loudspeaker support. Compared with a first-generation self-pressure-relief waterproof loudspeaker, the self-pressure-relief waterproof loudspeaker is simple in production process. The nuclear pore waterproof and breathable vibrating membrane achieves waterproof and breathable functions, is high in mechanical strength and good in toughness, and meets the requirements of deep waterproof products for toughness and high-pressure tearing of the waterproof and breathable membrane. The nuclear track membrane is good in air flow consistency, can meet the requirements of an electronic product with a built-in air pressure sensor and an electronic product for accurately measuring the current altitude, and has deep waterproof and air leakage functions.

A preparation method for a nuclear pore membrane having regularly arranged nuclear micropores, a radiation detector probe manufactured using the nuclear pore membrane, and a wave-absorbing material prepared using the nuclear pore membrane. Multiple single-pore nuclear pore membranes are used to prepare a dual-pore nuclear pore membrane having pores at a certain spacing, multiple dual-pore nuclear pore membranes are used to prepare a four-pore nuclear pore membrane having pores at a certain spacing and regularly arranged, and so on, a nuclear pore membrane having orderly arranged nuclear micropores is prepared using the principles of multiplication. An insulating material layer is sandwiched between two conductive material layers of a nuclear pore membrane or two sides of an insulating membrane are coated with conductive layers, and then orderly arranged nuclear micropores are formed, so as to obtain a radiation detector probe. Nuclear pore membranes having different pore sizes and taper angles are stacked and staggered to broaden the wavelength range of electromagnetic waves absorbed by the wave-absorbing material.

The invention discloses a gradient nuclear track membrane based on heavy ion irradiation and application of the gradient nuclear track membrane in cherry preservation, and belongs to the technical field of nuclear track membrane preparation. The method is used for solving the problems that in the prior art, PET films do not have pore diameters with moderate quantity and particle sizes, so that the PET films for storing cherries are too high in compactness and low in air permeability, and excessive anaerobic respiration exists during cherry storage. In addition, the technical problem that the PET film is poor in biological antibacterial activity is solved. The invention provides a gradient nuclear track membrane based on heavy ion irradiation for cherry preservation. The gradient nuclear track membrane based on heavy ion irradiation is composed of a nuclear track membrane substrate, and a first coating layer and a second coating layer which are sequentially coated on the surface of the nuclear track membrane substrate, the nuclear track membrane substrate is obtained by sequentially carrying out heavy ion irradiation, ultraviolet sensitization and alkaline etching liquid etching on a PET (Polyethylene Terephthalate) film; the first coating solution is obtained by dissolving copper zinc pyrithione and dopamine in ethanol, and the second coating solution is obtained by dissolving silver nitrate in ethanol.

The application relates to a preparation method and application of an electrochemical biosensor based on a MOF / nuclear pore membrane sandwich structure and belongs to the technical field of biosensing. The electrochemical biosensor based on the MOF / nuclear pore membrane sandwich structure comprises a nuclear pore membrane substrate, a MOF functional layer, a conductive enhancement layer and a biological recognition layer. The nuclear pore membrane substrate provides stable physical support for the MOF functional layer by taking the nuclear pore membrane as the substrate, simultaneously guides the directional transmission of target molecules along the pore channel and reduces diffusion resistance; the MOF functional layer is modified by in-situ growth of MOF materials on the inner wall of the nuclear pore membrane channel, the density of active sites is increased, then a continuous MOF layer is formed on the surface of the nuclear pore membrane, selective separation and sensing are realized and the current signal is amplified; the conductive enhancement layer is modified by a conductive material in the MOF channel to form a continuous conductive network, the electrode interface resistance is reduced, the transmission of electrons from the reaction site to the external circuit is accelerated, a conductive coating is prepared on the surface of the nuclear pore membrane as the electrode interface signal, the signal-to-noise ratio is reduced and the stability is improved; and the biological recognition layer is used for specific recognition of target molecules by fixing DNA probes on the surface of the MOF. The electrochemical biosensor based on the MOF / nuclear pore membrane sandwich structure can utilize the mechanical support and ordered pore channel of the nuclear pore membrane and improve the sensing performance through the functional surface of the MOF, and is suitable for high-sensitivity and high-selectivity biological molecule detection.

1. The name of the design product: nuclear pore membrane air conditioning window. 2. The use of the design product: for packaging box in the wet, temperature control, gas exchange. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention relates to a preparation method of an enzyme membrane sensor for detecting glutamine, and aims to provide a biosensor which can be matched with different redox electrodes for use, has general applicability and good performance and is suitable for online monitoring of glutamine. The enzyme membrane takes a polycarbonate filter membrane as a basement membrane, and is prepared by the following steps: fixing a nuclear track filter membrane on an O-shaped rubber ring, sequentially dropwise adding an enzyme solution on the filter membrane, and finally covering a layer of nuclear track filter membrane. The glutaminase membrane disclosed by the invention has relatively short response time and relatively stable operability, and meanwhile, the preparation process of the glutaminase membrane is simple and relatively low in cost.

The invention discloses a preparation method of a novel liposome for efficiently detecting and gathering bacteria, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: weighing phosphatidylcholine, dioleoyl phosphatidyldimethylalanine and cholesterol according to a molar ratio of POPC: DOTAP: Cholesterol of 3: 3: 4, dissolving in chloroform, carrying out rotary evaporation at 25 DEG C to form a lipid membrane, carrying out hydration, vortex and ultrasonic treatment by using PBS (Phosphate Buffer Solution) (pH 7.4), extruding by using a nuclear track membrane and a support membrane, and finally determining related parameters by using a dynamic light scattering technology. The lipidosome can be rapidly combined with bacteria, rapid detection and targeting of the bacteria are achieved, bacteria aggregation is promoted to reduce in-vivo diffusion, and the lipidosome is of great significance to early rapid detection and control of clinical bacteria.

The invention provides a nanopore nuclear track membrane rapid nondestructive detection method based on a dark field scattering optical method, which comprises the following steps: focusing a nuclear track membrane to be detected by using a dark field optical microscope, adjusting to a dark field mode after focusing, and obtaining a scattered light image of the nuclear track membrane to be detected in the dark field mode; determining the image area and the number of scattered light spots in the image based on the scattered light image, and obtaining the density of the nuclear track membrane; moving the light spot center of the dark-field optical microscope to the position of a single hole for dark-field scattering spectrum acquisition to obtain single-hole dark-field scattering spectrum intensity I; and based on a relational expression I = aD2 + b between the scattering spectrum intensity I and the aperture D, calculating the aperture of the nuclear pore model through the single-pore dark field scattering spectrum intensity I. Therefore, the method has the advantages of simplicity and convenience in operation, low cost, high detection speed and the like, and can be widely applied to the fields of nanotechnology and material science.

The application discloses a kind of nuclear pore membrane pore size automatic measurement method, by being plated to sample surface conductive film, to the sample after plating film is carried out electron microscope scanning, to scanning image sequentially do USM sharpening processing, corrosion inflation processing, hough circle detection processing, obtain nuclear pore membrane hole inside and outside diameter and its statistical distribution;The method of the application can detect all the pores of nuclear pore membrane, and the concentric circles, intersecting circles are detected by hough circle gradient detection algorithm and concentric circle processing algorithm, with high accuracy, the error is within 5% with artificial statistics;Using algorithm automatically operates, detection time is short, does not need manual operation, greatly improves detection accuracy;And simple architecture can be transplanted and run, improve application range.

The invention discloses an on-line haze feedback production method for a nuclear track membrane. The method comprises the following steps: controlling the advancing speed of a membrane material through a winding and unwinding device; etching the membrane material through an etching device to obtain a nuclear track membrane; cleaning the etching liquid on the nuclear track membrane through a cleaning device; drying the cleaned nuclear track membrane through a drying device; detecting the nuclear track membrane through haze detection equipment to obtain a real-time haze detection result and a real-time light transmittance detection result; and according to the real-time haze detection result and the real-time light transmittance detection result, operating parameters of the winding and unwinding device and the etching device are adjusted in real time. The method can achieve the real-time detection of the thickness and pores of the nuclear track membrane on a production line through haze and light transmittance detection, facilitates the automatic feedback production, is simple in detection method, and is high in detection speed. Equipment cost can be greatly saved, production efficiency is improved, and production waste is reduced.