The invention relates to a tracing and counting method for detecting quantity of exogenous microorganisms in soil. The tracing and counting method comprises the following steps: 1) preparing dye
stock solution and working solution; 2) activating and culturing
bacteria, centrifuging to obtain target
bacteria, fixing cells, adding dye working solution, and
dyeing in the dark place, so that dyed
bacteria are prepared; 3) adding the dyed bacteria into soil to be cultured, collecting a soil sample after culture, carrying out oscillating extraction to obtain the target bacteria, diluting, then filtering with a black
nuclear pore filter membrane, then placing on a
glass slide, and sealing the
glass slide; and 4) observing and counting under
ultraviolet light by adopting a fluorescent
microscope, and converting the quantity obtained by counting into the quantity of
microorganism cells contained in per kg of
dry soil. The tracing and counting method provided by the invention has the advantages that a fluorescent
dyeing counting technology is combined with a
fluorescent labelling technology, and growth and propagation conditions of the exogenous microorganisms added into the soil are traced and quantified; and the used fluorescent dye is convenient and fast to operate, extremely high in sensitivity, good in stability and extremely low in usage amount, so that a practical and reliable soil exogenous
microorganism tracing and counting method is provided.