20 results about "Nuclear pore" patented technology

The invention discloses a colorectal cancer Septin9 gene methylation detection kit based on a graded anchoring probe, and belongs to the technical field of molecular diagnosis. The graded anchoring probe is composed of a specific capture probe (F1), a precise anchoring probe (R1), a synergistic blocking probe (F2 / R2) and a short chain complementary sequence (S1) which are Septin9 methylation specific probes and can be fixed in a conical pore channel of a nuclear track membrane in a graded manner to form a capture-anchoring-blocking functional chain; during application, a general accumulation body is formed by combining with a Septin9 methylation target, a nuclear track membrane pore channel is blocked, current drop is caused, and detection is realized. Through cooperation of the probe, the nuclear track membrane and the electrochemistry, the problems of insufficient sensitivity, poor stability and poor specificity of traditional detection are solved, and the kit is suitable for ultra-early diagnosis of colorectal cancer.

The invention discloses an air freight nuclear track membrane humidity regulation and control fresh-keeping system and method, and the system comprises a cargo hold / container module which is an airplane main cargo hold or a container meeting the aviation standard, and is used for accommodating cargoes to be kept fresh and forming a closed microenvironment; the nuclear track membrane regulation and control layer is arranged in a mode of covering the ventilation opening of the cargo hold / container module, being integrated in the side wall structure or forming an independent humidity regulation buffer cabin; the nuclear track membrane regulation and control layer is composed of at least one layer of nuclear track membrane, and the nuclear track membrane is of a micropore structure which is uniform in pore diameter height and is of a straight-through pore channel. By introducing the intelligent molecular sieve, namely the nuclear track membrane, the system realizes dynamic bidirectional accurate regulation and control on the humidity in the aviation confined space. Compared with a traditional passive moisture absorption or complete sealing scheme, the system has the advantages that excessive moisture causing corruption can be actively removed, meanwhile, water molecules can be captured from a relatively dry airborne environment for humidification, and the two problems of moisture condensation and excessive drying are fundamentally solved.

The application discloses a method for directly preparing a nuclear micropore membrane with regularly arranged nuclear micropores based on a large-area AAO transmission film, and belongs to the technical field of nuclear micropore membrane preparation. The method comprises the following steps: taking a large-area through-hole anodic aluminum oxide (AAO) film as an ion transmission template, closely adhering the AAO film to a polymer substrate, and performing one-time vertical high-energy particle irradiation; particles penetrate along the regular channels of the AAO and form a large-area regular irradiation damage array on the substrate, which corresponds to the template; and through sensitization, chemical etching and post-processing, a nuclear micropore membrane with consistent nuclear micropore distances and regular arrangement is directly obtained. The method omits the traditional multiplication, alignment and multiple irradiation steps, is simple in process, high in product yield, and capable of large-area mass production, and is high in regularity of the nuclear micropore array, adjustable in parameters, and significantly improves the application performance of the nuclear micropore membrane in high-end fields such as radiation detection, wave absorption and high-precision separation.

The application discloses a fluorine removal agent and a preparation method thereof, and relates to the technical field of fluorine removal agents, and comprises the following components in parts by weight: 5-10 parts of an aluminum salt, 5-10 parts of an iron salt, 15-20 parts of a rare earth material, sodium citrate and beta-cyclodextrin; the addition amount of the sodium citrate is 5%-10% of the total mass of the aluminum salt, the iron salt and the rare earth material; and the addition amount of the beta-cyclodextrin is 30%-50% of the mass of the sodium citrate. Through the introduction of the synergistic system of sodium citrate and beta-cyclodextrin, the metal ion precipitation sequence is regulated, meanwhile, the beta-cyclodextrin captures competitive anions through molecular recognition and constructs a hydrogen bond network to block interference, and the combination of the microsphere type and the porous particle type further realizes dynamic directional transmission of pollutants and nuclear pore limited crystallization, and the active site distribution is reconstructed at the molecular level, so that a technical scheme with universality, economy and reliability is provided for the treatment of complex fluorine-containing wastewater.

The utility model provides a preservation box containing a nuclear track membrane. The preservation box comprises a box body, a cover body used for opening or closing the box body and a preservation mechanism used for controlled atmosphere preservation. The fresh-keeping mechanism comprises an inner window body, an outer window body detachably connected to the inner window body and an air adjusting assembly arranged between the inner window body and the outer window body, the inner window body is used for preventing liquid in the box body from entering the air adjusting assembly, and the outer window body is used for preventing liquid outside the box body from entering the air adjusting assembly; the air adjusting assembly is clamped and fixed through the inner window body and the outer window body. The air adjusting assembly comprises a front dust cover, a rear dust cover detachably connected to the front dust cover and a nuclear track membrane located between the front dust cover and the rear dust cover, and the nuclear track membrane is clamped and fixed through the front dust cover and the rear dust cover. The air conditioning device has the advantages of being stable in air conditioning function, convenient to maintain and suitable for household use.

The application discloses a nuclear pore membrane modified atmosphere packaging device production system, which comprises a mother piece feeding mechanism, a nuclear pore membrane cutting mechanism, a sub piece feeding mechanism and a multiple-speed chain conveying line. The automatic feeding of the mother piece and the sub piece is realized through the mother piece feeding device and the sub piece feeding device, and the automatic cutting of the nuclear pore membrane during the assembling process of the nuclear pore membrane and the sub piece is realized through the nuclear pore membrane cutting device. The overall assembling of the nuclear pore membrane modified atmosphere packaging device is realized to be automatic, the working efficiency of the assembling is obviously improved, and the qualified rate of the finished product is improved.

The invention relates to a high-fidelity expansion microscopic imaging method of a nuclear pore complex, which is characterized in that the signal intensity and the dyeing coverage are obviously enhanced through the immunostaining conditions of 37 DEG C temperature and prolonged incubation time, and the permeability of an antibody in iterative expansion gel is improved; by adopting a secondary anchoring cross-linking condition that 2.8% paraformaldehyde and 10% acrylamide are proportioned, under the condition that the expansion rate is not reduced, the structural heterogeneity index is further reduced, and the marking integrity is improved; through treatment of 92.5 mM SDS under the denaturation condition of 85 DEG C for 1.5 h, full denaturation can be achieved, and deformation and signal loss caused by protein breakage can be reduced. During imaging, the signal-to-noise ratio of fluorescence after single expansion is improved by using a water immersion objective lens, so that a clear annular NPC structure can be obtained by using a confocal microscope; therefore, the image fidelity and the signal-to-noise ratio are remarkably improved, and a technical basis is provided for subsequently realizing near-molecule-scale NPC imaging under a confocal microscope.

A preparation method for a nuclear pore membrane having regularly arranged nuclear micropores, a radiation detector probe manufactured using the nuclear pore membrane, and a wave-absorbing material prepared using the nuclear pore membrane. Multiple single-pore nuclear pore membranes are used to prepare a dual-pore nuclear pore membrane having pores at a certain spacing, multiple dual-pore nuclear pore membranes are used to prepare a four-pore nuclear pore membrane having pores at a certain spacing and regularly arranged, and so on, a nuclear pore membrane having orderly arranged nuclear micropores is prepared using the principles of multiplication. An insulating material layer is sandwiched between two conductive material layers of a nuclear pore membrane or two sides of an insulating membrane are coated with conductive layers, and then orderly arranged nuclear micropores are formed, so as to obtain a radiation detector probe. Nuclear pore membranes having different pore sizes and taper angles are stacked and staggered to broaden the wavelength range of electromagnetic waves absorbed by the wave-absorbing material.

1. The name of the design product: nuclear pore membrane air conditioning window. 2. The use of the design product: for packaging box in the wet, temperature control, gas exchange. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application discloses a kind of nuclear pore membrane pore size automatic measurement method, by being plated to sample surface conductive film, to the sample after plating film is carried out electron microscope scanning, to scanning image sequentially do USM sharpening processing, corrosion inflation processing, hough circle detection processing, obtain nuclear pore membrane hole inside and outside diameter and its statistical distribution;The method of the application can detect all the pores of nuclear pore membrane, and the concentric circles, intersecting circles are detected by hough circle gradient detection algorithm and concentric circle processing algorithm, with high accuracy, the error is within 5% with artificial statistics;Using algorithm automatically operates, detection time is short, does not need manual operation, greatly improves detection accuracy;And simple architecture can be transplanted and run, improve application range.

The invention discloses an on-line haze feedback production method for a nuclear track membrane. The method comprises the following steps: controlling the advancing speed of a membrane material through a winding and unwinding device; etching the membrane material through an etching device to obtain a nuclear track membrane; cleaning the etching liquid on the nuclear track membrane through a cleaning device; drying the cleaned nuclear track membrane through a drying device; detecting the nuclear track membrane through haze detection equipment to obtain a real-time haze detection result and a real-time light transmittance detection result; and according to the real-time haze detection result and the real-time light transmittance detection result, operating parameters of the winding and unwinding device and the etching device are adjusted in real time. The method can achieve the real-time detection of the thickness and pores of the nuclear track membrane on a production line through haze and light transmittance detection, facilitates the automatic feedback production, is simple in detection method, and is high in detection speed. Equipment cost can be greatly saved, production efficiency is improved, and production waste is reduced.

The utility model discloses a replaceable and detachable nuclear track membrane structure and an atomizer, and relates to the technical field of atomizers, the replaceable and detachable nuclear track membrane structure comprises a liquid bin, a boss is arranged in the middle of the liquid bin, an atomization groove is formed in the middle of the boss, a liquid storage cavity is formed between the liquid bin and the boss, a metal loading plate, an atomization nuclear track membrane and a piezoelectric ceramic ring are arranged in the atomization groove, and micropores are distributed in the center of the atomization nuclear track membrane. The boss is provided with a water drawing mechanism, the piezoelectric ceramic ring is connected with a power source, the atomization groove is provided with a clamping assembly matched with the atomization nuclear track membrane, the clamping assembly comprises a lifting pressing plate which is arranged in the atomization groove and connected with the piezoelectric ceramic ring, and the boss is provided with a displacement mechanism for promoting the lifting pressing plate to move in the vertical direction. A locking mechanism is arranged between the lifting pressing plate and the atomization nuclear track membrane and comprises an arc-shaped protrusion arranged on the atomization nuclear track membrane, and an arc-shaped groove is formed in the corresponding position of the lifting pressing plate. The atomization nuclear track membrane can be replaced conveniently, maintenance of the atomization nuclear track membrane is facilitated, the maintenance cost is low, and daily batch use is facilitated.

The invention discloses a composite nuclear track membrane as well as a preparation method and application thereof. The composite nuclear track membrane comprises a supporting base layer and a two-layer structure membrane formed on the supporting base layer through membrane scraping and finishing, wherein the thickness of the supporting base layer ranges from 50 micrometers to 120 micrometers, the thickness of the two-layer structure membrane ranges from 10 micrometers to 50 micrometers, and the two-layer structure membrane comprises a compact layer and a loose layer; the thickness of the compact layer is 100-200 nm, the aperture of the compact layer is 0-40 nm, the thickness of the loose layer is 10-50 [mu] m, and the aperture of the loose layer is 0.1-10 [mu] m; the composite nuclear track membrane is applied to the biological component separator. The composite nuclear track membrane as well as the preparation method and the application thereof aim at solving the problems of a dialysis membrane used by a biological component separator in the prior art so as to meet the requirement for accurate separation of biological substances in medical and biological experiments, and have important practical significance.

This invention relates to the field of molecular detection technology, specifically to a DNA tetrahedral probe for detecting lung cancer gene methylation. The probe is formed by the self-assembly of four single-stranded DNA strands S1-S4, with S1 extending into a recognition arm targeting the methylated fragment of the PTGER4 promoter region. This invention immobilizes the tetrahedral probe on the inner wall of a nuclear pore membrane, preparing it as the core electrochemical detection component of a reagent kit. Utilizing the specific recognition capabilities of the nuclear pore membrane and the probe, combined with electrochemical detection technology, rapid detection of PTGER4 gene methylation is achieved without the need for PCR nucleic acid amplification. This detection system is simple to operate, low in cost, and can be widely applied to screening high-risk groups for lung cancer.

The invention discloses a manufacturing method of a fresh-keeping cabinet composed of a phase-change material and a nuclear track membrane, and the manufacturing method comprises the following steps: S1, preparing a plastic box body with a containing cavity which is used for placing articles to be preserved; s2, an interlayer is arranged in the wall body of the plastic box body or an independent phase change material unit is arranged in the containing cavity, and the containing cavity is filled with a paraffin phase change material; s3, according to the size of the plastic box body, one or more ventilation holes of 5-10 cm are formed in the side wall or the top of the plastic box body; and S4, a layer of nuclear track membrane is attached to each vent hole, and the nuclear track membrane has the preset hole diameter and hole density so as to regulate and control the micro-atmosphere environment. By utilizing the latent heat characteristic of the paraffin phase-change material, the temperature in the fresh-keeping cabinet can be kept relatively stable within a certain period of time without an external power supply, energy consumption is remarkably reduced, and the fresh-keeping cabinet belongs to an environment-friendly technology and is particularly suitable for being used in regions with unstable electric power, outdoor operation, short-distance transportation or occasions without power supplies.

The utility model provides a nuclear track membrane fresh-keeping piece and a fresh-keeping bag, the fresh-keeping piece comprises a sub-piece and a mother piece which are in threaded fit with each other, a nuclear track membrane is arranged between the sub-piece and the mother piece, the sub-piece and the mother piece are clamped on the wall of a container through threaded connection and clamp and fix the nuclear track membrane, and the sub-piece and the mother piece enable the nuclear track membrane to be communicated with the inside and the outside of the container. The utility model has the advantages of simple installation and high stability, and is suitable for different containers.

The invention discloses a disposable electrochemical biosensor detection kit, and belongs to the technical field of in-vitro diagnosis. The kit is a disposable in-vitro diagnosis product and is divided into two product systems, namely a molecular detection system and an immunodetection system; the core components of the molecular detection kit are an integrated disposable H-type electrolytic cell, an isothermal amplification buffer solution, a positive control solution, a negative control solution and a blank control solution. The core components of the immunodetection kit are an integrated disposable H-type electrolytic cell, a detection reagent, a substrate reagent, a calibrator I, a calibrator II, a quality control product I and a quality control product II. The kit takes a functionalized nuclear track membrane as a core, the nuclear track membrane modifies corresponding target recognition probes in advance according to different detection targets, and the target recognition probes are pre-sealed and fixed in an H-shaped electrolytic cell to form a non-detachable integrated detection unit with the electrolytic cell; transmembrane transmission of a sample is realized through pressurization of a single inflation port, and high-precision detection can be realized by combining isothermal amplification or a double-antibody sandwich technology and an electrochemical sensing technology.

The invention discloses a waterproof breathable acoustic membrane based on PVDF crystal link regulation and control, a preparation method and wearable equipment, and relates to the technical field of waterproof breathable membranes. According to the preparation method, a PET nuclear track membrane substrate with a specific structure and a PVDF solution system regulated step by step are introduced, and a PET nuclear track membrane with a preformed microporous structure is selected as a rigid substrate, so that the problem of shrinkage and hemming caused by non-uniform solvent removal and stress concentration during pure PVDF film casting is solved. Two silane coupling agents with complementary functions are introduced step by step, so that precise regulation and control of PVDF crystallization behavior and interface combination are realized. According to the preparation method, excellent solution machinability of PVDF and excellent dimensional stability of the PET film are combined, so that parameters in the whole technological process are easy to control, repeatability is good, the technical bottleneck that a PVDF material is difficult to be directly used for preparing functional films with consistent forms on a large scale is successfully solved, and meanwhile, the defects that an ePTFE material is difficult to process and high in cost are overcome.

The utility model discloses a kind of nuclear pore membrane assembly recycling disassembling device, it is related to nuclear pore membrane assembly technical field, including bearing box and fixed plate, the bearing box side is fixed with rotating lever, the rotating lever other end is rotationally equipped with fixed rod, the fixed rod is fixedly equipped on mounting disc, the bearing box inside is equipped with clamping mechanism, the fixed plate is fixedly equipped in second electric cylinder output end, the fixed plate is sequentially equipped with dismounting mechanism and classification mechanism along the bearing box rotating direction. The utility model is intermittently rotated by setting several bearing boxes, and mounting disc drives several bearing boxes, so that mounting disc rotates a circle, the disassembly and classified storage of nuclear pore membrane assembly can be completed, and by setting clamping mechanism, clamping and releasing fixation to lower assembly are automatically completed in the process of bearing box rotation, and then it is convenient to continuously disassemble nuclear pore membrane assembly, thereby the practicability of nuclear pore membrane assembly recycling disassembling device is increased.

This invention discloses an immobilized oligonucleotide probe, a LAMP isothermal amplification system, a functionalized nuclear pore membrane, a nuclear pore membrane electrochemical biosensor, and a detection method thereof for recognizing methylation target sequences in the promoter region of the human RASSF1A gene using a nuclear pore membrane electrochemical biosensor. The immobilized oligonucleotide probe is fixed to the inner wall of the nanopores of a PET nuclear pore membrane. The synthesized methylation target sequence is captured on the inner wall of the nanopores through specific hybridization, and then undergoes in-situ LAMP amplification within the confined space of the nanopores. The accumulation of amplification products generates a pore-blocking effect. Cyclic voltammetry is used to detect changes in transmembrane ion current, thereby achieving the recognition and detection of the methylation target sequence. This invention has advantages such as well-defined targets, no need for fluorescent labeling, high detection integration, and applicability to the detection of synthetic nucleic acid samples.