Method for detecting human beta-globin gene mutation

A globin and human detection technology, applied in biochemical equipment and methods, microbial determination/inspection, fluorescence/phosphorescence, etc., can solve the problem of difficulty in simultaneous detection of multiple beta-globin gene mutations, cumbersome operations, and low throughput and other problems, to achieve the effect of saving reagents and costs, not easy to contaminate, and easy and fast to operate
CN101899499AActive Publication Date: 2010-12-01XIAMEN UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2010-12-01

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Abstract

The invention relates to a method for detecting human beta-globin gene mutation, in particular to a method for detecting the beta-globin gene mutation by a modified molecular beacon melting curve. The method comprises the following steps of: designing and preparing a corresponding modified molecular beacon in region of a beta-globin gene needing mutation detection; designing an upstream primer and a downstream primer on the periphery of the designed modified molecule beacon, and performing PCR amplification on fragments containing the region to be detected by using the upstream primer and the downstream primer; and after PCR amplification is finished, analyzing the melting curve, and judging whether a nucleotide sequence to be detected has the gene mutation and possible mutation types according to the change of the melting point of the modified molecular beacon.
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Description

technical field

[0001] The invention relates to a method for detecting human beta-globin gene mutation, in particular to a method for detecting beta-globin gene mutation by improving the molecular beacon melting curve. Background technique

[0002] β-thalassemia (β-thalassemia) is one of the most common single-gene recessive genetic diseases in humans. + ) or completely absent (β 0 ), thus the excess α-globin peptide chain combined with the red blood cell membrane eventually causes hemolytic anemia. The disease has been reported all over the world, and it is widely prevalent in some tropical and subtropical countries and regions, and most provinces and regions south of the Yangtze River in my country (including Taiwan, Hong Kong and Macao) are high-incidence areas of the disease.

[0003] Children with beta-thalassemia are usually healthy at birth but develop progressively between 6 months and 2 years of age, and if left undiagnosed and untreated, most die of anemia or inf...

Claims

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