Lyophilized reagent capable of simultaneously distinguishing three pathogen nucleic acids

Abstract

The invention discloses a lyophilized reagent for nucleic acid diagnosis. The lyophilized reagent comprises a lyophilized protecting agent which contains 2%-10% of trehalose, 2%-5% of glucan, 0.5%-2%of PEG8000 and 0.01mg-0.02mg / ml of BSA protein, and the lyophilized protecting agent contains mycoplasma pneumoniae, chlamydia pneumoniae and adenovirus primers and probes. The lyophilized reagent hasadvantages that since the lyophilized reagent contains three pathogen nucleic acids of mycoplasma pneumoniae, chlamydia pneumoniae and adenovirus, amplification detection of various pathogen nucleicacids can be realized, namely three targets can be simultaneously detected by one reaction system, and accordingly reagent and detection consumables are greatly saved, high detection throughput is realized, and a clinical application range is expanded. In application of the lyophilized reagent for lyophilization treatment, liquid nitrogen is adopted for quick pre-freezing of a liquid reagent, so that a subsequent lyophilization process is shortened, and stability of the lyophilized reagent is improved.

Description

Technical field [0001] The invention relates to the field of molecular biology, in particular to a freeze-dried reagent capable of distinguishing nucleic acids from three pathogens at the same time. Background technique [0002] PCR (Polymerase Chain Reaction) is a amplification and amplification technology for specific nucleic acid fragments. This technology is widely used in gene detection and pathogen detection. It has the characteristics of high sensitivity, good specificity, simplicity and speed. Medical research and clinical diagnosis are of great significance. [0003] Mycoplasma pneumonia (M.Pneumonia) is the pathogen of human Mycoplasma pneumonia. The pathological changes of mycoplasma pneumonia are mainly interstitial pneumonia, sometimes complicated by bronchial pneumonia, called primary atypical pneumonia, children are prone to severe pneumonia, so timely detection and medication is of great significance to the treatment of patients. [0004] Human Chlamydia pneumoniae ...

AI Technical Summary

Problems solved by technology

[0006] Traditional fluorescent PCR detection reagents used to detect Mycoplasma pneumoniae, Chlamydia pneumoniae, and adenovirus are mostly liquid single-plex detection reagents. One reaction system can only detect one target. The amount is low and cannot meet the needs of clinical testing

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