Method for preparing fluorescent carbon quantum dot

A technology for carbon quantum dots and fluorescence, which is applied in the field of preparing fluorescent carbon quantum dots, can solve the problems of low yield, long cycle and high cost, and achieves the effects of high fluorescence performance and improved fluorescence efficiency.

Inactive Publication Date: 2015-04-29
SINOCARE
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Problems solved by technology

[0005] At present, the methods for preparing carbon quantum dots with fluorescent properties include: high temperature and high pressure excision method; candle burning method; electrochemical scanning method; ch

Method used

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  • Method for preparing fluorescent carbon quantum dot
  • Method for preparing fluorescent carbon quantum dot
  • Method for preparing fluorescent carbon quantum dot

Examples

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Example Embodiment

[0064] The invention also provides a preparation method of the immunochromatographic test strip, including:

[0065] Step 1: Spray the antibody bound with fluorescent carbon quantum dots on the conjugate release pad to obtain the conjugate release area;

[0066] Step 2: Spray antigen and anti-mouse IgG antibody on the solid support in the reaction zone to form a test zone and a control zone to obtain the reaction zone;

[0067] Step 3: Paste the sample application area, conjugate release area, reaction area and water absorption area on the backing in turn.

[0068] Those skilled in the art can understand that the antibodies and antigens sprayed on the immunochromatographic test strips of the present invention can select antibodies and antigens suitable for different test substances.

[0069] The present invention also provides an immunochromatographic kit, including the fluorescent carbon quantum dots prepared by the preparation method.

[0070] In some preferred embodiments, the immunoc...

Example Embodiment

[0084] Embodiment 1. Detection method and calculation method of fluorescence quantum efficiency

[0085] The reference method was used to measure the fluorescence quantum efficiency. By selecting the wavelength corresponding to the intersection of the absorption curve of the standard solution and the analyte solution in the absorption spectrum as the excitation wavelength, the analyte and the standard are excited to obtain the corresponding fluorescence intensity. The fluorescence quantum efficiency of quinine sulfate in 0.05 mol / L sulfuric acid solution was selected as 55% as the standard, and the fluorescence quantum efficiency of fluorescent carbon quantum dots was calculated. Fluorescent carbon quantum dots fluorescence quantum efficiency The calculation formula is:

[0086]

[0087] Wherein (c) and (q) represent fluorescent carbon quantum dots and quinine sulfate respectively; A represents the absorption value at the excitation wavelength, I represents the fluorescence peak ...

Example Embodiment

[0088] Example 2. Preparation of fluorescent carbon quantum dots

[0089] Take 1g carrageenan and add 20g water to mix, place in a closed system, heat to 220-240℃, react for 4h, stop heating, cool to room temperature, centrifuge the reaction solution at 10000rpm for 30min, take the supernatant to obtain fluorescence Carbon quantum dots. The particle size of the fluorescent carbon quantum dots prepared by electron microscopy was about 2nm. According to the method described in Example 1, the fluorescence quantum efficiency of the prepared fluorescent carbon quantum dots is 35.5%. The prepared fluorescent carbon quantum dots of different concentrations were tested for the absorbance and fluorescence intensity at different wavelengths and the fluorescence intensity of the fluorescent carbon quantum dots in aqueous solution under different concentration gradients. The results are as follows Figure 2-4 .

[0090] by Figure 2-4 The results show that the absorbance and fluorescence i...

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Abstract

The invention belongs to the technical field of the immunochromatographic and in particular relates to a method for preparing a fluorescent carbon quantum dot, the prepared fluorescent carbon quantum dot and application of the prepared fluorescent carbon quantum dot to an immunochromatographic strip. According to the method for preparing the fluorescent carbon quantum dot, animal and plant colloids are used as raw materials to prepare the carbon quantum dot, the animal and plant colloid contains conjugated molecules such as benzene ring and can form a conjugated agglomerated carbon quantum dot in a reaction process, and the fluorescent carbon quantum dot has high fluorescence property; and furthermore, a polymer is added in a heating reaction process to effectively improve the fluorescence efficiency of the fluorescent carbon quantum dot; and experimental results show that the fluorescence quantum efficiency of the fluorescent carbon quantum prepared by the method can reach 30-50%.

Description

technical field [0001] The invention belongs to the technical field of immunochromatography, in particular to a method for preparing fluorescent carbon quantum dots, in particular to a method for preparing fluorescent carbon quantum dots, the prepared fluorescent carbon quantum dots and their application on immunochromatographic test strips . Background technique [0002] Immunochromatography is a unique immunoassay method that appeared in the early 1980s. It often uses strip-shaped fiber chromatography materials as the solid phase, and the sample solution swims on the chromatography strip through capillary action, and at the same time makes the sample solution The analyte and the receptor (such as antibody or antigen) for the analyte on the chromatographic material have a highly specific and high-affinity immune reaction, and the immune complex is enriched or trapped in the chromatographic material during the chromatography In a certain area (detection zone), an intuitive ...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N27/26
CPCG01N33/533
Inventor 吴天景
Owner SINOCARE
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