Method for preparing red and green light double-emission carbon dots and application

A dual-emission, green-light technology, applied in the chemical field, can solve problems such as cumbersome processes, and achieve the effects of good selectivity, low cytotoxicity, and high sensitivity

Active Publication Date: 2018-05-25
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Usually, the synthesis of traditional ratiometric fluorescent probes requires the chemical or physical combination of two materials with different fluorescence emission wavelengths, which is a cumbersome process.

Method used

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  • Method for preparing red and green light double-emission carbon dots and application
  • Method for preparing red and green light double-emission carbon dots and application
  • Method for preparing red and green light double-emission carbon dots and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Synthesis of RGDE CDs

[0034] Add 0.1g of 2,5-diaminotoluene sulfate and 1mL of absolute ethanol into a 20mL reaction kettle, react at 150°C for 12h, cool to room temperature, add 20mL of water to dilute, centrifuge at 12000r / min for 10min, and the supernatant is ready for RGDE CDs. Finally, the RGDE CDs solution was removed and stored in a refrigerator at 4°C for future use.

[0035] (2) The structure and morphology of RGDE CDs were characterized by TEM, XPS, FT-IR and other methods.

[0036] (3) The photobleaching resistance, thermal stability, salt tolerance and fluorescence intensity at different pH values ​​of RGDE CDs were investigated. Determine the measurement conditions and establish a ratiometric detection of OONO - new method of fluorescence.

[0037] (4) Detection of OONO by ratio method -

[0038](a) Add 30.0 μL RGDE CDs solution and 2.90 mL 10 mM Tris-HCl (pH=7.4) buffer solution into a centrifuge tube; add 70 μL of different concentrations of O...

Embodiment 2

[0055] Synthesis of RGDE CDs

[0056] Add 0.1g of 2,5-diaminotoluene sulfate and 0.5mL of absolute ethanol into a 20mL reaction kettle, react at 120-180°C for 12h, cool to room temperature, add 20mL of water to dilute, centrifuge at 12000r / min for 10min, and The supernatant is RGDE CDs. Finally, the RGDE CDs solution was removed and stored in a refrigerator at 4°C for future use.

Embodiment 3

[0058] Add 0.1g of 2,5-diaminotoluene sulfate and 2mL of absolute ethanol into a 20mL reaction kettle, react at 120-180°C for 12h, cool to room temperature, add 20mL of water to dilute, centrifuge at 12000r / min for 10min, and the supernatant The solution is RGDE CDs. Finally, the RGDE CDs solution was removed and stored in a refrigerator at 4°C for future use.

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Abstract

The invention provides water-soluble red/green light double-emission carbon dots (RGDE CDs) which are prepared by taking 2,5-diaminotoluene sulfate as a carbon source through a one-step method. Compared with an existing method, a multi-step material preparation process is avoided. The RGDE CDs prepared by the method have excellent anti-photo-bleaching capability, good biocompatibility and low cytotoxicity. Under the single excitation wavelength, the strength of green fluorescence (with the wavelength of 525nm) of the RGDE CDs is quenched after OONO- is added, and the strength of red fluorescence (with the wavelength of 603nm) is basically not changed. On the basis, the invention establishes a novel method for determining fluorescence of the OONO- through a ratio method.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a method for preparing red / green double-emitting carbon dots (RGDE CDs) in one step and an application thereof. Background technique [0002] Carbon dots (CDs) are discrete nanoparticles with a near-spherical structure, usually less than 10 nm in size, with photoluminescent properties. Since CDs were prepared by researchers using the arc discharge method in 2004, CDs have been used in analysis, detection, Bioimaging, photodynamic therapy, and photocatalysis have been widely used in fields such as carbon nanomaterials that have attracted much attention. [0003] Most of the CDs reported so far have short fluorescence emission wavelengths, poor tissue penetration, and large background interference of biological tissues, which limits their application in biological imaging and other aspects. The energy of red light is low, and the background interference of biological tissues ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15C09K11/65G01N21/64
CPCC01P2002/80C01P2002/82C01P2002/84C01P2004/04C09K11/65G01N21/6428G01N21/6456G01N2021/6432
Inventor 陈兴国刘娟娟董炎炎
Owner LANZHOU UNIVERSITY
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