A fluorescent carbon dot and its preparation method and application

A fluorescent carbon dot and fluorescent technology, applied in biochemical equipment and methods, chemical instruments and methods, nano-carbon, etc., can solve the problems of high price, poor photostability, complex dye synthesis, etc., and achieve low cost, simple preparation, Effect of wide distribution of fluorescence excitation spectrum and fluorescence emission spectrum

Active Publication Date: 2018-12-14
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The synthesis of these dyes is complex, expensive, and poor in photostability, and there is an urgent need for us to develop new mitochondrial fluorescent reagents with good photostability

Method used

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  • A fluorescent carbon dot and its preparation method and application
  • A fluorescent carbon dot and its preparation method and application
  • A fluorescent carbon dot and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The preparation of fluorescent carbon dots in this embodiment comprises the following steps:

[0039](1) Preparation of raw materials: Cultivate Staphylococcus aureus in LB medium to make its growth density reach 10 10 CFU / mL; Take a certain volume of cultured microbial culture fluid, centrifuge, resuspend and wash with pure water for 3 times, and finally suspend the microbial cell body in 30 mL of pure water, so that the mass ratio of bacteria to water is 1:1000;

[0040] (2) Reaction: React in a hydrothermal reactor at 200°C for 24 hours to form a carbon dot solution;

[0041] (3) Purification: Centrifuge or filter to obtain the target fluorescent carbon dot solution.

[0042] For a schematic diagram of the reaction, see figure 1 ; The transmission electron microscopy results of the prepared fluorescent carbon dots are shown in figure 2 ; The UV-Vis absorption spectrum of the prepared fluorescent carbon dots is shown in image 3 ; The fluorescence emission spectr...

Embodiment 2

[0044] The preparation steps of fluorescent carbon dots in this example are the same as those in Example 1, except that Staphylococcus aureus in step (1) is replaced by Escherichia coli.

[0045] For a schematic diagram of the reaction, see Figure 5 ; The transmission electron microscopy results of the prepared fluorescent carbon dots are shown in Figure 6 ; The UV-Vis absorption spectrum of the prepared fluorescent carbon dots is shown in Figure 7 ; The fluorescence emission spectra of the prepared fluorescent carbon dots under excitation of different wavelengths are shown in Figure 8 .

Embodiment 3

[0047] The preparation steps of fluorescent carbon dots in this embodiment are the same as in Example 1, except that Staphylococcus aureus in step (1) is replaced by Gram-negative bacteria such as Proteus vulgaris, Pseudomonas aeruginosa, etc.; and Gram bacteria such as Staphylococcus epidermidis and Micrococcus luteus.

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Abstract

The invention discloses a preparation method of fluorescent carbon dots. The preparation method comprises the following steps: (1) raw material preparation: bacteria growing to a plateau is cleaned centrifugally with pure water and resuspended in the pure water; (2) reaction: a reaction is performed at the temperature of 180-220 DEG C for 18-24 h; (3) purification: purification is performed after the temperature is reduced to the room temperature, and a fluorescent carbon dot solution is obtained. The invention further discloses applications of the prepared fluorescent carbon dots in fluorescence identification of viable and non-viable states of bacteria or fungi and in fluorescence targeted imaging of cell mitochondria. According to the preparation method, carbon dots with excellent fluorescence properties are prepared from bacteria as the raw material by one step with adoption of a hydrothermal reaction, have the advantages of good water solubility, biocompatibility, fluorescence radiation property and the like, not only can effectively identify the viable and non-viable states of microbes such as escherichia coli, staphylococcus aureus, Saccharomyces cerevisiae and the like, but also can specifically image the mitochondria of mammalian cells.

Description

technical field [0001] The invention belongs to bionano material technology, in particular to a fluorescent carbon dot and a method for synthesizing the fluorescent carbon dot based on microorganisms, and the application of the fluorescent carbon dot in the identification of the life and death of microorganisms and the fluorescence probe of cell mitochondria. Background technique [0002] Bacterial viability is an important indicator for evaluating disease infection, environmental pollution, food safety, and the efficacy of antibacterial drugs. It is of great practical significance to develop simple, efficient and accurate microbial viability detection methods. Common detection methods for bacterial viability mainly include plate colony counting method, turbidity method (absorbance detection method), microscope (including atomic force microscope, electron microscope and fluorescence microscope) observation method and spectrum (including Raman spectrum and infrared spectrum) d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15C12Q1/04C12Q1/02
CPCB82Y20/00B82Y40/00C01P2002/84C01P2004/04C01P2004/64C09K11/65C12Q1/04G01N33/5035
Inventor 吴富根华先武鲍琰雯
Owner SOUTHEAST UNIV
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