Quantum-dot immunochromatography detection card and detection method for detecting tadalafil medicine with ternary-system immune competition method
A ternary system, immunochromatography technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of ununiform preparation of signal substances, low detection signal strength and sensitivity, and insufficient stability, and it is beneficial to achieve active protection. , the detection time is short, the effect of increasing the stability of the antibody
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[0033] see figure 1 , which is the quantum dot immunochromatographic detection card for the detection of Lafite drugs by the ternary system immunocompetitive method of this embodiment, including PVC lining boards, and adjacent ones that are sequentially adhered to the PVC lining boards and partially overlapped Detect antigen release pad, quantum dot probe release pad, nitrocellulose membrane (NC membrane) and water-absorbing pad; The detection antigen release pad includes the detection agent that is formed by coupling OVA and lafilter drug (tadalafil) Antigen; the quantum dot probe release pad includes a quantum dot probe labeled with anti-OVA antibody; the nitrocellulose membrane is provided with a detection line and a quality control line, and the detection line is fixed with anti-diaphragm antibodies, so The above quality control line was fixed with OVA antigen.
[0034] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellul...
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