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Quantum-dot immunochromatography detection card and detection method for detecting tadalafil medicine with ternary-system immune competition method

A ternary system, immunochromatography technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of ununiform preparation of signal substances, low detection signal strength and sensitivity, and insufficient stability, and it is beneficial to achieve active protection. , the detection time is short, the effect of increasing the stability of the antibody

Pending Publication Date: 2018-10-26
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

3. HPLC and LC-MS can only detect known PDE-5 drugs. For the newly emerged PDE-5 analogues whose molecular structure is deliberately modified, due to the differences in chromatographic behavior and mass spectrometry behavior from known drugs, only liquid Phase / tandem ion trap mass spectrometry (LC / ESI-MS / MS) technology can detect
The "binary system immunocompetition method" has the following disadvantages: 1. It is necessary to label the signal substance for each detection antibody separately, so that the signal substance cannot be prepared uniformly; 2. The detection antibody is in the liquid phase and has insufficient stability; 3. The detection signal intensity and less sensitive

Method used

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  • Quantum-dot immunochromatography detection card and detection method for detecting tadalafil medicine with ternary-system immune competition method
  • Quantum-dot immunochromatography detection card and detection method for detecting tadalafil medicine with ternary-system immune competition method
  • Quantum-dot immunochromatography detection card and detection method for detecting tadalafil medicine with ternary-system immune competition method

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Embodiment Construction

[0033] see figure 1 , which is the quantum dot immunochromatographic detection card for the detection of Lafite drugs by the ternary system immunocompetitive method of this embodiment, including PVC lining boards, and adjacent ones that are sequentially adhered to the PVC lining boards and partially overlapped Detect antigen release pad, quantum dot probe release pad, nitrocellulose membrane (NC membrane) and water-absorbing pad; The detection antigen release pad includes the detection agent that is formed by coupling OVA and lafilter drug (tadalafil) Antigen; the quantum dot probe release pad includes a quantum dot probe labeled with anti-OVA antibody; the nitrocellulose membrane is provided with a detection line and a quality control line, and the detection line is fixed with anti-diaphragm antibodies, so The above quality control line was fixed with OVA antigen.

[0034] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellul...

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Abstract

The invention relates to a quantum-dot immunochromatography detection card and detection method for detecting tadalafil medicine with a ternary-system immune competition method. The detection card comprises a liner plate, a detection antigen releasing pad, a quantum-dot probe releasing pad, a chromatography film and a water absorption pad; the detection antigen releasing pad comprises a detectionantigen formed by coupling albumin eggs and the tadalafil medicine; the quantum-dot probe releasing pad comprises a quantum dot probe marked with an anti-albumin-egg antibody; the chromatography filmis provided with a detection line and a quality control line, the detection line fixes the antibody of the tadalafil medicine, and the quality control line fixes the albumin-egg antigen. The detectionantigen is dissolved into a detection system from the releasing pad, a ternary-system immune complex is formed by the detection antigen, the detection antibody and the marked antibody, and fluorescence detection signals are generated. Under the detection reference effect of the quality control line, whether the tadalafil medicine is contained in a sample or not is judged according to the fluorescence signals on the detection line, the signal strength and the stability of detection are improved, and the detection sensitivity and the quantitative accuracy are increased.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health care products and Chinese patent medicines, especially the quantum dot immune layer for detection of Latina drugs by the ternary system immune competition method Analysis test card and test method. Background technique [0002] In the early 1990s, a PDE-5 inhibitor drug used by Pfizer for the treatment of coronary heart disease was found to be effective in the treatment of impotence in clinical trials. This is sildenafil citrate. After that, the other two The PDE-5 inhibitor drugs Bayer's vardenafil and Eli Lilly's tadalafil have successively produced huge commercial interests in the market. [0003] Driven by huge market interests, criminals synthetically illegally add various PDE-5 inhibitors to natural aphrodisiac products to increase the aphrodisiac e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/543
CPCG01N33/533G01N33/5436
Inventor 郭杰标钟瑞敏章仕杰钟林涛谢伟光蒋昊
Owner SHAOGUAN COLLEGE
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