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Fluorescence nanometer probe and preparation method thereof

A fluorescent nanoprobe and phospholipid technology, applied in the field of nanomedicine, can solve problems such as low stability and insufficient biocompatibility

Active Publication Date: 2012-07-04
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional ICG-based fluorescent nanoprobes are not stable and have insufficient compatibility with organisms.

Method used

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  • Fluorescence nanometer probe and preparation method thereof
  • Fluorescence nanometer probe and preparation method thereof
  • Fluorescence nanometer probe and preparation method thereof

Examples

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preparation example Construction

[0031] A method for preparing the above-mentioned fluorescent nanoprobes, comprising the steps of:

[0032] Step S1: provide ICG and PLGA, prepare ICG solution and PLGA solution respectively, and then mix ICG solution and PLGA solution to obtain a mixed solution of ICG and PLGA.

[0033] Among them, the ICG solution is preferably an aqueous solution of ICG with a concentration of 0.5-2 mg / mL. The PLGA solution is preferably a PLGA acetonitrile solution with a concentration of 1-5 mg / mL.

[0034] During the mixing process, measure the corresponding volume of ICG solution and PLGA solution according to the volume ratio of 1:5-20 and mix them.

[0035] Step S2: Provide phospholipids and DSPE-PEG-NH 2 (or DSPE-PEG-COOH), prepare phospholipids with DSPE-PEG-NH 2 (or DSPE-PEG-COOH) mixed solution. Specifically include the following steps:

[0036] Dissolving phospholipids in a mixed solvent of chloroform and methanol with a volume ratio of 8 to 10:1 to obtain a phospholipid sol...

Embodiment 1

[0044] (1), respectively prepare ICG aqueous solution with a concentration of 1 mg / mL and PLGA acetonitrile solution with a concentration of 2 mg / mL, and ultrasonically mix 100 μL of ICG aqueous solution and 1 mL of PLGA acetonitrile solution to obtain a mixed solution of ICG and PLGA;

[0045] (2) Weigh 0.24mg of soybean lecithin and dissolve it in a chloroform-methanol solvent with a volume ratio of 9:1 to obtain a phospholipid solution, then weigh 0.06mg of DSPE-PEG-COOH, add the phospholipid solution and DSPE-PEG-COOH into 3 mL of 4% ethanol aqueous solution, heated to 65°C and stirred for 3 min to obtain a mixed solution of phospholipids and DSPE-PEG-COOH;

[0046] (3) Add the mixed solution of ICG and PLGA dropwise to the mixed solution of phospholipid and DSPE-PEG-COOH to react, and stir continuously at 35°C for 4h, during which the solvent is allowed to evaporate, and the fluorescent nanoprobe with an average diameter of 95.7nm is obtained. needle, such as figure 2 ,...

Embodiment 2

[0048] (1), respectively prepare an ICG aqueous solution with a concentration of 1 mg / mL and a PLGA acetonitrile solution with a concentration of 2 mg / mL, and ultrasonically mix 50 μL of the ICG aqueous solution with 1 mL of the PLGA acetonitrile solution to obtain a mixed solution of ICG and PLGA;

[0049] (2) Weigh 0.24mg of soybean lecithin and dissolve it in a chloroform-methanol solvent with a volume ratio of 9:1 to obtain a phospholipid solution, then weigh 0.06mg of DSPE-PEG-COOH, add the phospholipid solution and DSPE-PEG-COOH into 3 mL of 4% ethanol aqueous solution, heated to 65°C and stirred for 3 min to obtain a mixed solution of phospholipids and DSPE-PEG-COOH;

[0050] (3) Add the mixed solution of ICG and PLGA dropwise to the mixed solution of phospholipids and DSPE-PEG-COOH to react, stir continuously at 35°C for 4h, and allow the solvent to volatilize during this period to obtain fluorescent nanoprobes with an average diameter of 84.8nm Needle.

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Abstract

The invention relates to a fluorescence nanometer probe, comprising an inner core formed by polyglycolide lactide, a middle layer formed by phospholipid surrounding on the surface of the inner core and a shell formed by distearoyl phosphatidyl ethanolamine-polyethylene glycol which contains amino or carboxyl and partially penetrates through the middle layer, wherein indocyanine green is dispersed in the inner core. According to the invention, a core-shell structure is formed to ensure that the indocyanine green is wrapped in the polyglycolide lactide, therefore, the indocyanine green is effectively avoided from being aggregated and decomposed and the stability is increased; and the wrapped indocyanine green has a near-infrared fluorescence characteristic to ensure that the background fluorescence penetrating tissues is small and then can be relatively accurately applied to bioluminescence labeling.

Description

【Technical field】 [0001] The invention relates to the field of nano-medicine, in particular to a fluorescent nano-probe and a preparation method thereof. 【Background technique】 [0002] Indocyanine Green (ICG) is a near-infrared fluorescent dye approved by the US Food and Drug Administration for clinical diagnosis. It has a characteristic absorption peak in the near-infrared region and can be used in the field of bioluminescence labeling. However, the stability of ICG is poor, and it will quickly aggregate and decompose in polar solvents, and the decomposition will be accelerated under light conditions. Therefore, researchers have conducted a lot of research to improve its stability so that it can be used for fluorescent labeling. For example, Kirchherr et al. (Kirchherr A K, Briel A, and Mader K.Stabilization of Indocyanine Green by Encapsulation within MIcellar Systems[J].Molecular pharmaceuticals, 2009, 6(2):480-491.) wrap ICG in the micellar system Prepared ICG micelle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/02
Inventor 蔡林涛郑翠芳郑明彬龚萍贾冬雪
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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