8 results about "Ethanolamine synthesis" patented technology

The application discloses a liposome of a FOXO1 inhibitor AS1842856 and a preparation method and application thereof. The liposome comprises the following raw materials: phospholipid, cholesterol, phospholipid PEG derivative and AS1842856; wherein the proportion of the phospholipid, the cholesterol and the phospholipid PEG derivative (for example, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 (DSPE-PEG2000)) is preferably in the range of 10:0.2-2:0.5-5 (w / w); wherein the AS1842856 is wrapped in the liposome. The AS1842856 liposome of the application has excellent physical and chemical properties and has a wide application prospect in the field of fibrosis disease treatment.

The present application relates to the technical field of medicine, in particular to a kind of composition for treating IDH1 mutation and mutant drug resistance and application thereof, the composition is composed of AG120 and phosphatidyl ethanolamine (PE).The present application finds that the composition containing AG120 and PE can synergistically reduce the expression of IDH1 mutant carcinogenic metabolite 2-HG and inhibit tumor growth;And improve the therapeutic effect after IDH1 mutant solid tumor is resistant to AG120, can reduce the expression of IDH1 mutant drug-resistant tumor cell carcinogenic metabolite 2-HG and inhibit its tumor growth;The effect of combination group is obviously superior to the group of AG120 used alone.At the same time, the pharmaceutical composition is not found in the process of application treatment that mouse weight reduces and obvious drug toxicity.

The present application relates to the field of medicine, and particularly relates to a liposome loaded with phagolysosomal peptide, a preparation method and application thereof, so as to provide a drug for treating pancreatitis, and meanwhile improve the bioavailability of the drug. The liposome loaded with phagolysosomal peptide comprises phagolysosomal peptide and a shell coated on the periphery of the phagolysosomal peptide, the shell comprises an inner layer and an outer layer, the shell component is distearoyl phosphatidyl ethanolamine-polyethylene glycol, and there is a selenium-selenium bond between the inner layer and the outer layer. The inventor of the present application first discloses the direct effect and mechanism of the phagolysosomal peptide on the damaged pancreas of SAP, and the prepared DSSM@TN liposome can inhibit P2X7-induced mitochondrial damage, accumulation of ROS and expression of NLRP3, improve the bioavailability of the phagolysosomal peptide, significantly enhance the efficacy of the phagolysosomal peptide, and further improve the potential of the phagolysosomal peptide in preventing and treating SAP in the clinic. The DSSM@TN liposome can be used as a drug for treating pancreatitis, and meanwhile provides a new direction for the treatment research of pancreatitis.

The application discloses a kind of loaded manganese doped ceria nanoscale enzyme's Pachyman hydrogel and its preparation method and application, it is related to biomedical technical field.The preparation method is first prepared by high-temperature thermal decomposition method fat-soluble manganese doped ceria nanoscale enzyme, then it is surface-modified using distearoyl phosphatidyl ethanolamine to obtain water-soluble nanoscale enzyme;Polyvinyl alcohol solution is mixed uniformly with Pachyman powder, water-soluble nanoscale enzyme, borax solution is added to crosslink and form hydrogel, after freeze-room temperature processing alternately, it is obtained.The energy storage modulus of the prepared hydrogel is 120-350 Pa at pH 6.5-7.2, 37 ℃, and the adhesion is greater than or equal to 8.5 kPa.The hydrogel prepared by the application has good biocompatibility, can efficiently remove active oxygen, relieve intestinal oxidative stress, and has suitable adhesion and degradation characteristics, can be administered by enema, and be used for preparing drugs for treating irritable bowel syndrome.

The application belongs to the technical field of biological pigment reconstruction and protein engineering, and particularly relates to a pigment complex based on recombinant macrobrachium rosenbergii lipid carrier protein as well as an in-vitro preparation method and application thereof. The formation of the pigment complex relies on the synergistic stabilization of a key residue network of the recombinant macrobrachium rosenbergii lipid carrier protein, a pigment characteristic end group and phosphatidyl ethanolamine. The application has important scientific significance and industrial value for revealing the protein-pigment-lipid ternary interaction rule, constructing a thermal response color-changing system, expanding the application of protein-based pigment materials and providing a technical basis for color biomimetic construction in food and other systems.

The application discloses a method for improving the stability and bioavailability of wheat peptide, and constructs a wheat peptide delivery system through a nano-liposome encapsulation technology. The wheat peptide has various biological activities, but has the problems of poor stability, easy degradation in a physiological environment and low bioavailability. The application uses L-alpha-phosphatidyl ethanolamine and cholesteryl chloroformate as raw materials, and the optimized conditions are that the mass ratio of PE to Chol is 1:5, and the peptide-encapsulating amount is 30%. The peptide-encapsulating liposome is prepared through the steps of dissolving, injecting, ultrasonicating and rotary evaporating. The liposome has a particle size of 235.4+ / -1.18 nm, a PDI of 0.20+ / -0.02, a stable system, uniform particle size, an encapsulation rate of 95.16% and a loading rate of 67.55%. Structural characterization proves that the wheat peptide is successfully encapsulated, and the two form a stable supramolecular structure through non-covalent interaction. Compared with free wheat peptide, the stability of the wheat peptide in the pH, temperature, salt ion fluctuation and simulated gastrointestinal fluid is significantly improved, the in-vitro antioxidant activity is greatly enhanced, an innovative scheme is provided for oral delivery of the wheat peptide, and the application has important scientific value and industrial potential.

The application discloses a kind of alpha-lipoic acid loaded polylactic acid composite nanoparticles and preparation method and application thereof.The nanoparticles are formed with polylactic acid-glycolic acid copolymer to form a hydrophobic inner core, with distearoyl phosphatidyl ethanolamine-polyethylene glycol- triphenylphosphine to form a surface functional layer, and alpha-lipoic acid is loaded. The system has the advantages of mild preparation conditions, uniform particles, high drug loading efficiency, slow release in gastric acid environment, sustained release in neutral or weak acid environment, good cell compatibility, and enhanced mitochondrial regional positioning. The nanoparticles can reduce hypoxia-induced reactive oxygen species and Ca²⁺ elevation, improve ATP deficiency, and reduce myocardial and lung tissue damage, and are suitable for preparing pharmaceutical preparations for alleviating hypoxia-induced mitochondrial dysfunction and related organ damage.

The application relates to the field of material chemical synthesis, in particular to a synthesis method of large-size colloidal gold microspheres, which comprises the following steps: dissolving oleylamine and chloroauric acid in toluene, heating to boiling and continuously reacting for 3 hours, adding ethanol for precipitation after cooling to room temperature, centrifuging to obtain oil-phase colloidal gold nanoparticles OA-AuNPs, and redissolving with toluene to obtain OA-AuNPs toluene solution; mixing and dissolving the OA-AuNPs toluene solution obtained in the step S1 with distearoyl phosphatidyl ethanolamine-polyethylene glycol-n-heptanoic acid, adding sodium dodecyl sulfate SDS aqueous solution, and forming a microemulsion through ultrasonic emulsification. The synthesis method is efficient, easy to prepare and stable in performance; the prepared large-size colloidal gold microspheres are large in loading capacity, excellent in colorimetric characteristics, strong in stability, have rich functional groups on the surface which can be directly coupled with proteins, and overcome the related defects of traditional colloidal gold.