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Composite membrane containing aquaporin and manufacturing method thereof

An aquaporin and composite membrane technology, applied in the field of preparing separation membrane composite materials, can solve problems such as poor stability, low composite membrane strength, and reduced water passing efficiency, and achieve high composite strength, high water permeability, and long service life. Effect

Active Publication Date: 2013-12-25
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the bond between the adsorbed phospholipid bilayer membrane and the basement membrane only depends on the hydrophilic force, which makes the strength of this composite membrane low and the stability in actual operation is poor.
In the composite membrane encapsulating vesicles, water molecules need to pass through aquaporin twice, which reduces the efficiency of water passage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0014] A composite membrane containing aquaporin and a preparation method thereof, comprising the following steps:

[0015] (1) Blow dry 5ml of 2mg / ml chloroform solution of 1,2-dioleoylphosphatidylethanolamine with nitrogen gas and dry it, add 20ml of PBS buffer solution with pH=8.0, vibrate and sonicate to prepare 1,2- Dioleoylphosphatidylethanolamine vesicles;

[0016] (2) Add 1ml of PBS buffer solution of aquaporin 1 (0.015 mmol / ml) / glycerol (0.1mg / ml) / n-octyl-β-D-glucopyranoside (0.01mg / ml), mix The solution was put into a dialysis bag with a molecular weight cut-off of 7KDa, and then dialyzed in PBS buffer solution for 3 days to obtain aquaporin / 1,2-dioleoylphosphatidylethanolamine vesicles;

[0017] (3) Put the polysulfone-based membrane in 100ml of dopamine hydrochloride (2mg / ml) Tris buffer solution with pH=8.0, and shake it at 30°C for 24 hours, then wash it repeatedly with ethanol and deionized water to obtain Polysulfone-based membrane of polydopamine;

[0018] ...

Embodiment example 2

[0021] A composite membrane containing aquaporin and a preparation method thereof, comprising the following steps:

[0022] (1) Blow dry 5ml of 2mg / ml chloroform solution of 1,2-dioleoylphosphatidylethanolamine with nitrogen gas and dry it, add 20ml of Tris buffer solution with pH=8.0, vibrate and sonicate to prepare 1,2- Dioleoylphosphatidylethanolamine vesicles;

[0023] (2) Add 2ml of PBS buffer solution of aquaporin 1 (0.015 mmol / ml) / glycerol (0.1mg / ml) / n-octyl-β-D-glucopyranoside (0.01mg / ml) and mix The solution was put into a dialysis bag with a molecular weight cut-off of 7KDa, and then dialyzed in PBS buffer solution for 3 days to obtain aquaporin / 1,2-dioleoylphosphatidylethanolamine vesicles;

[0024] (3) Put the polysulfone-based membrane in 100ml of dopamine hydrochloride (2mg / ml) Tris buffer solution with pH=8.0, and shake it at 30°C for 24 hours, then wash it repeatedly with ethanol and deionized water to obtain Polysulfone-based membrane of polydopamine;

[00...

Embodiment example 3

[0028] A composite membrane containing aquaporin and a preparation method thereof, comprising the following steps:

[0029] (1) Blow dry 5ml of 2mg / ml 1,2-dimyristoylphosphatidylethanolamine in chloroform with nitrogen gas and dry it, add 20ml of Tris buffer solution with pH=8.0, vibrate and sonicate to prepare 1,2 - dimyristoylphosphatidylethanolamine vesicles;

[0030] (2) Add 1ml of PBS buffer solution of aquaporin 1 (0.015 mmol / ml) / glycerol (0.1mg / ml) / n-octyl-β-D-glucopyranoside (0.01mg / ml), mix The solution was put into a dialysis bag with a molecular weight cut-off of 7KDa, and then dialyzed in PBS buffer solution for 3 days to obtain aquaporin / 1,2-dioleoylphosphatidylethanolamine vesicles;

[0031] (3) Put the polysulfone-based membrane in 100ml of dopamine hydrochloride (2mg / ml) Tris buffer solution with pH=8.0, and shake it at 30°C for 24 hours, then wash it repeatedly with ethanol and deionized water to obtain Polysulfone-based membrane of polydopamine;

[0032] (...

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Abstract

The invention discloses a manufacturing method of a composite membrane containing aquaporin. The manufacturing method comprises the steps that dopamine is auto-polymerized on the surface of a base membrane, and the base membrane with polymerized dopamine attached to the surface and amidogen of a phosphatidyl ethanolamine membrane containing aquaporin are made to react, and therefore the composite membrane is manufactured. According to the manufactured composite membrane, covalent bonds exist between a functional layer and the base membrane, composite strength is high, etching resistance and hydrolysis resistance are strong, service life is long, surface hydrophilicity is good water permeability is high, the manufacturing condition is mild, and the composite membrane has wide application prospects in the field of water treatment and membrane separation.

Description

technical field [0001] The invention relates to a composite membrane containing water channel protein and a preparation method thereof, belonging to the field of preparing separation membrane composite materials. Background technique [0002] Desalination of seawater is one of the effective ways to solve the shortage of water resources, and membrane separation technology is the most effective and economical means to achieve desalination of seawater. The design of high-performance separation membranes is the foundation and core of membrane technology. The preparation of new composite membranes to effectively reduce energy consumption and realize efficient and convenient water purification technology is the current consensus and future development direction in the field of membrane material research. . Aquaporin is an ideal channel for water molecules, which has extremely strong water permeability and single selective permeability. Wrapping aquaporin in a phospholipid bilaye...

Claims

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Application Information

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IPC IPC(8): B01D69/12B01D67/00B01D71/60B01D71/68
Inventor 王志宁王熙大王淼琪丁万德高从堦
Owner OCEAN UNIV OF CHINA
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