Method for performing supersensitive detection on fungaltoxin of DTNB mark-based gold@silver core-shell nanorod

Abstract

The invention relates to a method for performing supersensitive detection on fungaltoxin of a DTNB mark-based gold@silver core-shell nanorod, and belongs to the technical fields of food safety, environment monitoring and the like. The gold nanorod is prepared by a seed growing method, 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) is marked on the gold nanorod, meanwhile, the gold nanorod is coated with a silver shell layer to prepare a gold@DTNB@silver surface Raman enhanced substrate, a superparamagnetic magnetic material chitosan ferroferric oxide is prepared, a fungaltoxin aptamer complementary chain is coupled on the prepared Raman enhanced substrate, a fungaltoxin aptamer chain is coupled on the magnetic material, an enhancer and the magnetic material are combined and a Raman signal of a system is strongest when the fungaltoxin does not exist in the detection system, and the aptamer modified magnetic material is specifically and preferentially combined with the fungaltoxin and the Raman signal of the system is changed after an external magnetic field is separated when the fungaltoxin exists, so that the aim of quantitatively detecting the fungaltoxin is fulfilled.

Description

technical field [0001] The invention relates to methods applicable to technical fields such as food safety and environmental monitoring, and in particular to a method for supersensitive detection of mycotoxins based on DTNB-labeled gold@silver core-shell nanorods. Background technique [0002] Mycotoxins are common microbial toxins in grain foods such as corn and peanuts. They are toxic secondary metabolites produced by fungi growing in food or feed, and have the potential to cause liver cancer, gastric cancer, kidney cancer, and intestinal cancer. At present, conventional detection methods for mycotoxins mainly include biological identification methods, but this method can only be used for qualitative detection, and the specificity is not strong, and the sensitivity is low; chemical analysis method (thin layer chromatography), the precision is poor , it is difficult to apply in practice; instrumental methods (gas chromatography, liquid chromatography, GC-MS, LC-MS), but the...

AI Technical Summary

Problems solved by technology

At present, conventional detection methods for mycotoxins mainly include biological identification methods, but this method can only be used for qualitative detection, and the specificity is not strong, and the sensitivity is low; chemical analysis method (thin layer chromatography), the precision is poor , it is difficult to apply in practice; instrumental methods (gas chromatography, liquid chromatography, GC-MS, LC-MS), but the pretreatment is complex and the instruments are expensive; immunoassays (enzyme-linked immunosorbent assay, immunofluorescence technique , radioimmunoassay, etc.), but this method has some false positives

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