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Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules

A technology of two-dimensional nanostructures and small molecules, which is used in the preparation methods of peptides, nanotechnology, nanotechnology, etc.

Inactive Publication Date: 2013-02-06
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there are few reports on the use of peptide assembly to form two-dimensional ordered nanostructures

Method used

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  • Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules
  • Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules
  • Method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules

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Embodiment 1

[0022] Example 1 is based on scanning probe microscopy and transmission electron microscopy to observe the two-dimensional nanosheet structure formed by the co-assembly of surfactant-like polypeptides and regulatory molecules, and to study the mechanical properties of polypeptide nanosheets using a quantitative nanomechanical imaging system.

[0023] 1. The chemical structure of the substance used

[0024] Polypeptide molecules (NH 2 -KKKFAFAFAFAKKK-COOH), the chemical structure of 4,4’-bipyridine-like molecule (4Bpy) is shown below:

[0025]

[0026] Surfactant-like peptides

[0027]

[0028] 4'4-bipyridine (4'4-bipyridyl, 4Bpy)

[0029] 2. Specific method

[0030] 1) Design a surfactant-like polypeptide containing 8 hydrophobic groups, of which 8 amino acids are FAFAFAFA, 4 phenylalanine (F) and 4 alanine (A), in order to regulate the water solubility of the polypeptide 3 lysines (K) are designed at the two ends of the polypeptide respectively, and the final polype...

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Abstract

The invention relates to a method for forming two-dimensional polypeptide nanosheets by regulating assembly of polypeptides with organic micromolecules, belonging to the technical field of biological materials. The method comprises the following steps: (1) selecting a molecule self-assembly polypeptide, and selecting an organic micromolecule regulator; (2) thoroughly mixing the polypeptide molecule and the regulator molecule to form a mixed solution; and incubating at 37 DEG C for 7 days. The molecule regulator is introduced on the molecular level to regulate the polypeptides to form a two-dimensional self-assembly sheet structure. The scanning tunnel microtechnique on the molecular level observes that the regulator molecule can regulate the surfactant-like polypeptide assembly structure. The invention provides the interaction site between the regulator molecule and polypeptide on the molecular level, so that people can clearly understand the polypeptide assembly mechanism under the regulation of the regulator molecule.

Description

technical field [0001] The invention belongs to the technical field of biomaterials. Specifically, the invention relates to a method for introducing regulator molecules to regulate polypeptide assembly and then form two-dimensional polypeptide nanosheets. Background technique [0002] As we all know, molecular self-assembly has become a very effective way to prepare nanostructures. It can induce molecules to form highly ordered nanostructures through various specific interactions between molecules without external interference. Especially in biology and biology-related fields, this technology is widely used. Some biomolecules or biomolecules, such as polypeptides and proteins, can use intermolecular hydrogen bond interactions, hydrophobic interactions, etc. to self-assemble into one-dimensional or zero-dimensional nanostructures, such as one-dimensional nanofibers, nanotubes; zero-dimensional nanostructures ball. These nanostructures can be widely used in various fields. ...

Claims

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Application Information

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IPC IPC(8): C07K14/00C07K1/02B82Y5/00
Inventor 刘磊董明东
Owner JIANGSU UNIV
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