Animal origin free flocks and herds frozen semen diluent and production method of flocks and herds frozen semen

A technology for freezing semen and a production method, which is applied in the fields of biological and chemical sciences, can solve the problems of restricting the popularization of animal artificial insemination technology, increasing labor for preparing diluents, wasting semen diluents, etc., so as to promote promotion, improve labor productivity, reduce The effect of labor

Inactive Publication Date: 2008-11-26
辽宁省重大动物疫病应急中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The oversimplified single storage method of semen diluent limits the promotion of animal artificial insemination technology to a large extent, increases the labor of preparing diluent, wastes semen diluent, and affects the improvement of labor productivity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Prepare animal semen diluent:

[0034] Use an electronic balance to weigh 0.85 grams of fructose, 3.10 grams of sucrose, 3.75 grams of lactose, 0.58 grams of trehalose, 1.80 grams of ethylenediaminetetraacetic acid, 2.45 grams of trimethylolaminomethane, nitrogen-trimethylolaminoethane sulfonate 0.015 grams of acid, 0.003 grams of chromium acetate, 2.0ml of phosphate buffer solution (no calf serum components), V E Sodium selenite injection solution 0.30ml, phosphate buffer 3.0ml, cysteine ​​0.05g, ethylene glycol 2.50ml, propylene glycol 3.50ml, erythritol 0.50ml, mannitol 0.50ml, cholesterol 0.30ml, add bis Distilled water 3.0ml. Mix the above substances thoroughly, cool immediately after heating and boiling, then add double distilled water to keep the volume of the original substance before heating and boiling, stir vigorously, use double distilled water to fully mix with the original drug solution, and then add the following substances: Phosphatidylserine 2.18 g...

Embodiment 2

[0044] 1) Prepare animal semen diluent:

[0045] Use an electronic balance to weigh 0.85 grams of fructose, 3.10 grams of sucrose, 3.75 grams of lactose, 0.58 grams of trehalose, 1.80 grams of ethylenediaminetetraacetic acid, 2.45 grams of trimethylolaminomethane, nitrogen-trimethylolaminoethane sulfonate 0.015g acid, 0.003g chromium acetate, 2.0ml phosphate buffer (no calf serum), 3.0ml phosphate buffer, 0.05g cysteine, 6.00ml ethylene glycol, 0.50ml erythritol, manna Alcohol 0.50ml, cholesterol 0.30ml, add double distilled water 3.0ml. Mix the above substances thoroughly, cool immediately after heating and boiling, then add double distilled water to keep the volume of the original substance before heating and boiling, stir vigorously, use double distilled water to fully mix with the original drug solution, and then add the following substances: Phosphatidylserine 2.18 grams, 2.00 grams of soluble soybean protein, 0.25 grams of soybean lecithin, vitamin B1 0.18 g, vitamin ...

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PUM

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Abstract

A cattle and sheep non-animal-derived frozen semen diluent comprises at least one or the combination of the following ingredients: trehalose, propylene glycol, soybean lecithin, soluble soybean protein, nitrogen-trishydroxymethyl aminoethanesulfonic acid and dodecanoyl sodium sulfate. A production method of a cattle and sheet frozen semen by using the cattle and sheep non-animal-derived frozen semen diluent fully mixes the cattle or sheet semen and the dilutent, lowers the temperature and carries out the preservation. Compared with the prior art, the animal semen dilutent of the invention eliminates the animal-derived ingredients; the preservation method is more practical, the technical effects are better, thus thoroughly eliminating the potential risks of spreading various epidemic diseases between the animals and the people by the animal-derived ingredients in the dilutent. The cattle and sheep non-animal-derived frozen semen diluent can promote the popularization of animal artificial insemination technology to the utmost extent and improve the labor productivity and the economic benefits, thus having great economic and social values.

Description

Technical field: [0001] The invention relates to biological and chemical sciences, and in particular provides a diluent of cattle and sheep semen prepared with no animal source components and a method for making frozen semen of cattle and sheep with the diluent. Background technique: [0002] In the prior art, the composition of various animal semen dilutions has not yet got rid of the level of development that completely deviates from animal-derived components, and the preservation methods of semen dilutions mainly remain at the level of ready-to-use or frozen storage. In such a situation as described above, there must be a potential risk of transmission of various diseases between animals and humans through components of animal origin in the dilution. The oversimplified single method for preserving the semen diluent limits the popularization of animal artificial insemination technology to a large extent, increases the labor of preparing the diluent, wastes the semen diluen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/52A01N1/02
Inventor 张世伟宋先忱刘兴伟韩迪张文军豆兴堂杨文凯
Owner 辽宁省重大动物疫病应急中心
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