Method for preparing 2 type diabetes rat model

A type 2 diabetes and rat model technology, applied in preparations for in vivo experiments, pharmaceutical formulas, animal feed, etc., can solve the problem of long modeling time, failure to reach clinical diagnosis of diabetes, and low fasting blood sugar in T2DM rat models and other problems, to achieve the effect of high model success rate and shortened molding time

Inactive Publication Date: 2008-01-02
FIRST HOSPITAL OF SHANXI MEDICAL UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0012] However, the modeling time of the above methods is generally relatively long, and the T2DM rat models prepared by some methods have low fasting blood glucose, which cannot meet the standards for clinical diagnosis of diabetes.

Method used

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  • Method for preparing 2 type diabetes rat model
  • Method for preparing 2 type diabetes rat model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] 1. Experimental materials

[0034] 1. Main medicines and kits

[0035] Streptozotocin (STZ), American Sigma Company;

[0036] Citrate, Boster;

[0037] Serum triglyceride (TG) test kit, serum cholesterol (Tch) test kit, Shanghai Rongsheng Biotechnology Co., Ltd.;

[0038] Plasma insulin kit (radioimmunoassay), French CIS company;

[0039] Glycosylated hemoglobin (microcolumn method) assay kit, Ningbo Cicheng Biochemical Reagent Factory;

[0040] Reagents related to Masson staining, Department of Pathology, Shanxi Medical University;

[0041] Blood glucose test strips, American Johnson & Johnson;

[0042] Urine glucose test strips, Guilin Zhonghui Biotechnology Co., Ltd.

[0043] 2. Main instruments

[0044] Johnson & Johnson sure step plus blood glucose meter, Johnson & Johnson;

[0045] UV755B ultraviolet-visible spectrophotometer, Shanghai Analytical Instrument General Factory;

[0046] LD4-2A desktop centrifuge, Beijing Medical Centrifuge Factory;

[0047] ...

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Abstract

The invention relates to a method for preparing type-2 diabetes mouse model, which comprises following steps: using streptozocin to make the reaction of islets of pancreas beta cell of new-born mouse to sugar be reduced, injecting treptozocin to weaning mouse to injure islets of pancreas lightly, feeding mouse with high-sugar high-fat food to enable it resist to insulin, and getting type-2 diabetes mouse model. The type-2 diabetes mouse model is characterized by typical symptom of high fasting blood-glucose concentration, high insulinemia, reduced sugar-resistant property and insulin-resistant property. The time for preparing model is shortened and the success rate is high.

Description

technical field [0001] The invention relates to a preparation method of an animal model, in particular to a preparation method of a type 2 diabetes rat model. The type 2 diabetes rat model prepared by the invention can be used for analyzing type 2 diabetes pathology and screening type 2 diabetes drugs. Background technique [0002] Diabetes is an ancient and young disease. As early as BC, my country's "Huangdi Neijing" discussed its etiology, disease and treatment principles in detail. With the development of society and the abundance of materials, the energy intake of the vast majority of people has changed from a long-term relative deficiency to a relative surplus in a short period of time. Thrify genotype, which is the main mission, is difficult to change in a short period of time, and still plays an inherent role, causing the incidence of diabetes to rise sharply, posing a severe challenge to current medicine. [0003] Diabetes is the second killer of modern diseases, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A23K1/18A23K10/30A23K10/37A23K20/158A23K20/163A23K50/00
CPCY02P60/87
Inventor 王东文双卫兵张利高宏飞
Owner FIRST HOSPITAL OF SHANXI MEDICAL UNIV
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