Method for establishing type 2 diabetes animal model and application of type 2 diabetes animal model in screening of blood sugar reducing medicaments

A type 2 diabetes and animal model technology, which is applied in applications, animal feed, animal feed, etc., can solve problems such as unsuccessful modeling, lack of sodium cholate, and inability to reflect the pathogenic impact of type 2 diabetes

Inactive Publication Date: 2012-12-12
NORTHEAST NORMAL UNIVERSITY
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Problems solved by technology

[0005] Studies have shown that type 2 diabetes has an important relationship with diet and alcohol consumption, and the above model cannot reflect the impact of diet and alcohol on the pathogenesis of type 2 diabetes
[0006] It has been reported that high-fat, high-sugar and alcohol combined to build an animal model of type 2 diabetes, due to the defects in its high-fat and high-sugar formula, the lack of sodium cholate (to promote the absorption of cholesterol and fat in mice), resulting in some indicators in the animal model Not statistically significant, modeling unsuccessful

Method used

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  • Method for establishing type 2 diabetes animal model and application of type 2 diabetes animal model in screening of blood sugar reducing medicaments

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Experimental program
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[0016] 1. Mouse test:

[0017] Thirty C57BL mice were randomly divided into two groups, 15 in each group. The normal group was fed with normal feed, free to eat and drink; the experimental group was fed with high-fat and high-sugar feed, free to drink, and fed 0.2 ml of 30% edible alcohol every day.

[0018] The proportion of high-fat and high-sugar feed is 60% of basic feed; 15% of sucrose; 15% of fat; 3% of cholesterol; 0.5% of sodium cholate; 6.5% of egg yolk powder.

[0019] After feeding for 8 weeks, they were fasted during the day, that is, fasted at 6:00 in the morning, and blood was taken by decapitation at 18:00 in the evening, and serum was separated, and various indicators in the blood were detected by an automatic biochemical analyzer. Statistical software was used to analyze the significance of the data.

[0020] The results are shown in Table 1. Compared with the normal group, the fasting blood glucose, insulin, cholesterol, triglyceride, and low-density lipopr...

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Abstract

The invention provides a method for establishing a type 2 diabetes animal model. An animal is fed with a high-fat and high-sugar feed for a period of time, and the animal is drenched with alcohol with certain concentration at the same time. The attack process of human type 2 diabetes is simulated in the method; and the prepared animal model has typical characteristics of the type 2 diabetes such as high fasting blood glucose, high insulinemia, hyperlipidemia and impaired glucose tolerance, and is suitable for mechanism research of the type 2 diabetes and screening of a large quantity of medicaments.

Description

technical field [0001] The present invention relates to a method for preparing an animal model of type 2 diabetes and its application in drug screening for diabetes, in particular to a method for preparing an animal model for analyzing the pathogenesis of type 2 diabetes and its use in screening type 2 diabetes-related drugs application in animal models. Background technique [0002] Diabetes, known as diabetes in traditional Chinese medicine, is an endocrine disease mainly caused by metabolic disorders of sugar, fat, and protein caused by the absolute or relative insufficient secretion of insulin in the body. It is one of the chronic diseases that seriously endanger the society and human health. [0003] In recent years, the incidence of diabetes has been on the rise. According to the statistics of the World Health Organization, the number of diabetes cases reached 135 million in 1998, 160 million in 2000, and 240 million in 2004. According to recent epidemiological surve...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K1/18A23K1/16
Inventor 孙成新范玉莹李新志
Owner NORTHEAST NORMAL UNIVERSITY
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